Molecular characterisation of bacteria in a wetland used to remove ammoniacal-N from landfill leachate

Molecular characterisation of bacteria in a wetland used to remove ammoniacal-N from landfill leachate

Bacteria associated with leachate from a constructed, surface-flow wetland effective at removing ammonium-N, were characterised using molecular methods which bypass the need for cultivation. DNA was extracted from the sample and bacterial 16S rDNA sequences amplified and cloned. Ninety-six individua...

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Bibliographic Details
Published in:Waste management & research Vol. 20; no. 6; pp. 529 - 535
Main Authors: Walsh, Kerry A., Hill, Tom C.J., Moffett, Bruce F., Harris, James A., Shaw, Phil J., Wallace, Joanne S.
Format: Journal Article
Language:English
Published: Thousand Oaks, CA SAGE Publications 01-12-2002
Subjects:
Alcaligenes
artificial wetlands
DNA, Bacterial - genetics
Ecosystem
Polymerase Chain Reaction
Proteobacteria - genetics
Proteobacteria - isolation & purification
Refuse Disposal
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
Soil Microbiology
Soil Pollutants
16S rDNA
wetland
passive treatment
ARDRA
landfill leachate
sequencing
wmr 503-6
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Summary:Bacteria associated with leachate from a constructed, surface-flow wetland effective at removing ammonium-N, were characterised using molecular methods which bypass the need for cultivation. DNA was extracted from the sample and bacterial 16S rDNA sequences amplified and cloned. Ninety-six individual clones were re-amplified and analysed by restriction digestion and targeted sequencing. A collector's curve was used to estimate the total number of bacterial groups (operational taxonomic units, OTUs) in the sample at 47. Thirty-six clones representing 28 OTUs were sequenced and characterised by aligning them against published sequences. Of these, 10 were confidently identified, whilst for the remaining 18, the closest match was obtained. The sequences in 64% of the OTUs were similar to those of the Proteobacteria but no classical nitrifying bacteria were identified. However, alkaliphilic Methylomicrobium sp. that oxidise ammonium and bacteria from the genus Alcaligenes, at least one species of which is capable of heterotrophic nitrification, were detected.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0734-242X
1096-3669
DOI:10.1177/0734242X0202000606