Evaluation of the safety and immunogenicity of the oral inactivated multivalent enterotoxigenic Escherichia coli vaccine ETVAX in Bangladeshi adults in a double-blind, randomized, placebo-controlled Phase I trial using electrochemiluminescence and ELISA assays for immunogenicity analyses

Highlights•The killed oral ETEC vaccine ETVAX ± dmLT adjuvant was safe in Bangladeshi adults. •All vaccinees responded to all 5 primary vaccine antigens in ALS specimens. •A majority of vaccinees responded to ≥4 antigens in plasma specimens. •A sensitive electrochemiluminescence assay was establishe...

Full description

Saved in:

Bibliographic Details
Published in:	Vaccine Vol. 37; no. 37; pp. 5645 - 5656
Main Authors:	Akhtar, Marjahan, Chowdhury, Mohiul I, Bhuiyan, Taufiqur R, Kaim, Joanna, Ahmed, Tasnuva, Rafique, Tanzeem A, Khan, Arifuzzaman, Rahman, Sadia I.A, Khanam, Farhana, Begum, Yasmin A, Sharif, Mir Z, Islam, Laila N, Carlin, Nils, Maier, Nicole, Fix, Alan, Wierzba, Thomas F, Walker, Richard I, Bourgeois, A. Louis, Svennerholm, Ann-Mari, Qadri, Firdausi, Lundgren, Anna
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier Ltd 03-09-2019 Elsevier Limited Elsevier Science
Subjects:	Adult Adults Age Age groups Allergy and Immunology Antibodies Antibodies in lymphocyte supernatant Antibody-secreting cell Antigens Assaying b-subunit vaccine Bacteria Children cholera vaccination Colonization developing-countries Diarrhea Double-blind studies E coli Electrochemiluminescence ELISA Enzyme-linked immunosorbent assay Escherichia coli ETEC etec vaccine factors humans IgA Immunogenicity Immunoglobulin A Immunoglobulins Immunologi inom det medicinska området Immunology Immunology in the medical area infants intestinal immune-responses Intestine Lymphocytes Microbiology in the medical area Mikrobiologi inom det medicinska området mucosal Randomization Safety Secretions Statistical analysis Vaccine Vaccines Bangladesh colonization factors LCTB A IgA ALS LT AEs cholera toxin B-subunit ASC serious adverse event Adult secretory IgA CTB/LTB hybrid protein MSD peripheral blood mononuclear cells ST heat labile toxin Vaccine Electrochemiluminescence adverse events ETEC PBMC heat-stable toxin ECL CTB CFs enterotoxigenic Escherichia coli SAE SIgA Antibody-secreting cell Antibodies in lymphocyte supernatant double mutant heat labile toxin Meso Scale Discovery dmLT ELISA LCTBA
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Highlights•The killed oral ETEC vaccine ETVAX ± dmLT adjuvant was safe in Bangladeshi adults. •All vaccinees responded to all 5 primary vaccine antigens in ALS specimens. •A majority of vaccinees responded to ≥4 antigens in plasma specimens. •A sensitive electrochemiluminescence assay was established for small sample volumes. •ALS responses measured by electrochemiluminescence and ELISA assays correlated well.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 ObjectType-News-3 content type line 23
ISSN:	0264-410X 1873-2518
DOI:	10.1016/j.vaccine.2018.11.040