Search Results - "Shang, Guangdong"

Homing endonuclease I-SceI-mediated Corynebacterium glutamicum ATCC 13032 genome engineering by Wu, Meng, Xu, Yan, Yang, Jun, Shang, Guangdong

“…Corynebacterium glutamicum is widely used to produce amino acids and is a chassis for the production of value-added compounds. Effective genome engineering…”
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Combination of ssDNA recombineering and CRISPR-Cas9 for Pseudomonas putida KT2440 genome editing by Wu, Zhixin, Chen, Zhongqiu, Gao, Xinyue, Li, Jing, Shang, Guangdong

“…Pseudomonas putida KT2440 is a Gram-negative, biosafety strain that plays important roles in environmental and biotechnological applications. Highly efficient…”
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A novel piperidine identified by stem cell-based screening attenuates pulmonary arterial hypertension by regulating BMP2 and PTGS2 levels by Xing, Yanjiang, Zhao, Shuang, Wei, Qingxia, Gong, Shiqiang, Zhao, Xin, Zhou, Fang, Ai-Lamki, Rafia, Ortmann, Daniel, Du, Mingxia, Pedersen, Roger, Shang, Guangdong, Si, Shuyi, Morrell, Nicholas W, Yang, Jun

“…Genetic defects in bone morphogenetic protein type II receptor (BMPRII) signalling and inflammation contribute to the pathogenesis of pulmonary arterial…”
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Gene silencing of myofibrillogenesis regulator-1 by adenovirus-delivered small interfering RNA suppresses cardiac hypertrophy induced by angiotensin II in mice by Dai, Wenjian, He, Weiqing, Shang, Guangdong, Jiang, Jiandong, Wang, Yiguang, Kong, Weijia

“…Our previous studies proved that myofibrillogenesis regulator (MR)-1 has a close relationship with cardiac hypertrophy induced by ANG II. In the present study,…”
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Construction and functional characterization of an integrative form λ Red recombineering Escherichia coli strain by Song, Jie, Dong, Huiqing, Ma, Chao, Zhao, Biyu, Shang, Guangdong

“…λ Red recombineering is a DNA cloning and engineering technique involving recombination between homologous regions. The homologous recombination is mediated by…”
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Cloning, expression and functional analysis of nicotinate dehydrogenase gene cluster from Comamonas testosteroni JA1 that can hydroxylate 3-cyanopyridine by Yang, Yao, Chen, Ting, Ma, Pengjuan, Shang, Guangdong, Dai, Yijun, Yuan, Sheng

“…A nicotinate dehydrogenase (NaDH) gene cluster was cloned from Comamonas testosteroni JA1. The enzyme, termed NaDHJA₁, is composed of 21, 82, and 46 kDa…”
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The Post-Polyketide Synthase Modification Steps in the Biosynthesis of the Antitumor Agent Ansamitocin by Actinosynnema pretiosum by SPITELLER, Peter, BAI, Linquan, SHANG, Guangdong, CARROLL, Brian J., YU, Tin-Wein, FLOSS, Heinz G.

“…The functions of six genes in the ansamitocin biosynthetic gene cluster of Actinosynnema pretiosum have been investigated by gene inactivation and chemical…”
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Cloning, heterologous expression, and functional characterization of the nicotinate dehydrogenase gene from Pseudomonas putida KT2440 by Yang, Yao, Yuan, Sheng, Chen, Ting, Ma, Pengjuan, Shang, Guangdong, Dai, Yijun

“…6-Hydroxynicotinate can be used for the production of drugs, pesticides and intermediate chemicals. Some Pseudomonas species were reported to be able to…”
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Rapid Degradation of Hazardous Amides by Immobilized Engineered Pseudomonas putida KT2440 Based on a Novel Gene Expression Vector by Jiang, Huoyong, Yu, Xuexiu, Guo, Jingjing, Shang, Guangdong, Dai, Yijun

“…The amides 4-trifluoromethylnicotinamide, acrylamide, and benzamide are widely used in agriculture and industry, posing hazards to the environment and animals…”
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Construction of asm2 deletion mutant of Actinosynnema pretiosum and medium optimization for ansamitocin P-3 production using statistical approach by BANDI, Srinivasulu, KIM, Yoonjung, CHANG, Yong-Keun, GUANGDONG SHANG, YU, Tin-Wein, FLOSS, Heinz G

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Recombineering and I-SceI-mediated Pseudomonas putida KT2440 scarless gene deletion by Chen, Zhongqiu, Ling, Wen, Shang, Guangdong

“…Pseudomonas putida KT2440 is a saprophytic and generally recognized as safe microorganism that plays important roles in the biodegradation and production of…”
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Escherichia coli BL21(DE3) optimized deletion mutant as the host for whole-cell biotransformation of N‑acetyl‑d‑neuraminic acid by Zhang, Qiong, Zhang, Jiao, Shao, Yanhong, Shang, Guangdong

“…N ‑Acetyl‑ d ‑neuraminic acid (Neu5Ac) is the crucial compound for the chemical synthesis of antiflu medicine Zanamivir. Chemoenzymatic synthesis of Neu5Ac…”
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CRISPR/Cas9-assisted ssDNA recombineering for site-directed mutagenesis and saturation mutagenesis of plasmid-encoded genes by Zhang, Guoyi, Wang, Junyu, Li, Yiwen, Shang, Guangdong

“…Site-directed and saturation mutagenesis are critical DNA methodologies for studying protein structure and function. For plasmid-based gene mutation, PCR and…”
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Characterization of a novel Escherichia coli recombineering selection/counterselection cassette by Zhang, Guoyi, Zhang, Qiong, Wang, Junyu, Zhang, Jiao, Shang, Guangdong

“…Recombineering is a highly efficient DNA cloning and modification technique by using the recombinase-mediated homologous recombination…”
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Recombineering-Mediated Sinorhizobium meliloti Rm1021 Gene Deletion by Yang, Jun, Zhang, Qiong, Zhang, Guoyi, Shang, Guangdong

“…Sinorhizobium meliloti Rm1021 ( S. meliloti Rm1021) is a Gram-negative, soil-dwelling α-proteobacterium which serves as a model microorganism for the studies…”
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Coupling ssDNA recombineering with CRISPR-Cas9 for Escherichia coli DnaG mutations by Li, Jing, Sun, Jian, Gao, Xinyue, Wu, Zhixin, Shang, Guangdong

“…Homologous recombination-based recombineering is a widely used DNA cloning and modification technique; recombineering efficiency improvement would be helpful…”
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Production of N‑Acetyl‑d‑neuraminic Acid by Whole Cells Expressing Bacteroides thetaiotaomicron N-Acetyl‑d‑glucosamine 2‑Epimerase and Escherichia coli N-Acetyl‑d‑neuraminic Acid Aldolase by Gao, Xinyue, Zhang, Feifei, Wu, Meng, Wu, Zhixin, Shang, Guangdong

“…N-Acetyl-d-neuraminic acid (Neu5Ac) is a potential baby nutrient and the key precursor of antiflu medicine Zanamivir. The Neu5Ac chemoenzymatic synthesis…”
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Pseudomonas putida KT2440 markerless gene deletion using a combination of λ Red recombineering and Cre/loxP site-specific recombination by Luo, Xi, Yang, Yunwen, Ling, Wen, Zhuang, Hao, Li, Qin, Shang, Guangdong

“…Pseudomonas putida KT2440 is a saprophytic, environmental microorganism that plays important roles in the biodegradation of environmental toxic compounds and…”
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Construction and physiological studies on a stable bioengineered strain of shengjimycin by Guangdong, S, Jianlu, D, Yiguang, W

“…Shengjimycin is a group of 4"-acylated spiramycins with 4"-isovalerylspiramycin as the major component, produced by recombinant S. spiramyceticus F21 harboring…”
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Characterization of Inducible ccdB Gene as a Counterselectable Marker in Escherichia coli Recombineering by Zhang, Qing, Yan, Zhenya, Xu, Yan, Sun, Jian, Shang, Guangdong

“…Recombineering is a homologous-based DNA cloning and modification technique. Recombineering-mediated chromosomal gene knock-in usually involves a…”
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