Reactivity of anti-nipah virus monoclonal antibodies to formalin-fixed, paraffin-embedded lung tissues from experimental nipah and hendra virus infections

Reactivity of anti-nipah virus monoclonal antibodies to formalin-fixed, paraffin-embedded lung tissues from experimental nipah and hendra virus infections

The immunohistochemical reactivity of seven clones of mouse monoclonal antibodies raised to Nipah virus antigens were investigated using formalin-fixed, paraffin embedded porcine and equine lung tissues from experimental Nipab and Hendra virus infection, respectively. Either microwave irradiation or...

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Bibliographic Details
Published in:Journal of Veterinary Medical Science Vol. 66; no. 10; pp. 1263 - 1266
Main Authors: Tanimura, N. (National Inst. of Animal Health, Tsukuba, Ibaraki (Japan)), Imada, T, Kashiwazaki, Y, Shamusudin, S, Syed Hassan, S, Jamaluddin, A, Russell, G, White, J
Format: Journal Article
Language:English
Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01-10-2004
Japan Science and Technology Agency
Subjects:
Animals
Antibodies, Monoclonal - immunology
Antibodies, Viral - immunology
Hendra virus
Henipavirus Infections - diagnosis
Henipavirus Infections - immunology
HISTOPATHOLOGY
Horses
Immunoenzyme Techniques
Immunohistochemistry
IMMUNOLOGICAL TECHNIQUES
Lung - pathology
Lung - virology
Mice
MONOCLONAL ANTIBODIES
monoclonal antibody
Nipah virus
Nipah Virus - immunology
PARAMYXOVIRIDAE
SWINE
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Summary:The immunohistochemical reactivity of seven clones of mouse monoclonal antibodies raised to Nipah virus antigens were investigated using formalin-fixed, paraffin embedded porcine and equine lung tissues from experimental Nipab and Hendra virus infection, respectively. Either microwave irradiation or enzymatic digestion effectively unmasked the viral antigens in formalin-fixed, paraffin-embedded tissue sections. Four clones showed positive reaction to both Nipah virus-infected porcine lung tissue and Hendra virus-infected equine lung tissue. Two clones (11F6 and 13A5) reacted with Nipah virus-infected porcine lung tissue, but not with Hendra virus-infected equine lung tissue. These Nipah virus-specific monoclonal antibodies may therefore be useful for imrnunohistological diagnosis of Nipah virus infection and for further research on Nipah virus pathogenesis.
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2005004275
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ISSN:0916-7250
1347-7439
DOI:10.1292/jvms.66.1263