Search Results - "Shamlou, P. Ayazi"

Design of a prototype miniature bioreactor for high throughput automated bioprocessing by Lamping, S.R, Zhang, H, Allen, B, Ayazi Shamlou, P

“…A new miniature bioreactor with a diameter equal to that of a single well of a 24-well plate is described and its engineering performance as a fermenter…”
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Impact of engineering flow conditions on plasmid DNA yield and purity in chemical cell lysis operations by Meacle, F.J., Lander, R., Ayazi Shamlou, P., Titchener-Hooker, N.J.

“…Chemical lysis of bacterial cells using an alkaline solution containing a detergent may provide an efficient scalable means for selectively removing covalently…”
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Ultra scale-down approach for the prediction of full-scale recovery of ovine polycolonal immunoglobulins used in the manufacture of snake venom-specific Fab fragment by Neal, G., Christie, J., Keshavarz-Moore, E., Shamlou, P. Ayazi

“…In this article, we describe a new approach that allows the prediction of the performance of a large‐scale integrated process for the primary recovery of a…”
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Formation of LID vector complexes in water alters physicochemical properties and enhances pulmonary gene expression in vivo by JENKINS, R. G, MENG, Q-H, HODGES, R. J, LEE, L. K, BOTTOMS, S. E. W, LAURENT, G. J, WILLIS, D, AYAZI SHAMLOU, P, MCANULTY, R. J, HART, S. L

“…There is currently an urgent need to develop efficient gene-delivery systems for the lung that are free of inflammatory effects. The LID vector is a synthetic…”
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Preparation of small unilamellar vesicles (SUV) and biophysical characterization of their complexes with poly-l-lysine-condensed plasmid DNA by Maguire, Leigh A., Zhang, Hu, Shamlou, P. Ayazi

“…Liposomes have numerous applications in the (bio)pharmaceutical industries as agents in the synthesis of new biomaterials for use in areas including gene…”
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Characterisation of the physical stability of colloidal polycation-DNA complexes for gene therapy and DNA vaccines by Lee, L.K, Mount, C.N, Ayazi Shamlou, P

“…Plasmid DNA is a potentially effective and safe means of in vivo delivery of exogenous genetic material to human cells and tissues. The lack of physical…”
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Time Course of SDS–alkaline lysis of recombinant bacterial cells for plasmid release by Ciccolini, L. A. S., Shamlou, P. Ayazi, Titchener‐Hooker, N. J., Ward, J. M., Dunnill, P.

“…SDS–alkaline lysis of recombinant Escherichia coli cell suspensions was carried out in a coaxial cylinder rheometer, and the data were used to establish the…”
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The effects of material properties and fluid flow intensity on plasmid DNA recovery during cell lysis by Levy, M.S., Ciccolini, L.A.S., Yim, S.S.S., Tsai, J.T., Titchener-Hooker, N., Ayazi Shamlou, P., Dunnill, P.

“…The disruption of recombinant E. coli cells containing a 76.8 kb plasmid DNA was achieved by the chemical lysis method in a coaxial cylinder rheometer which…”
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Vibrating membrane filtration for recovery and concentration of insect killing nematodes by Wilson, J.A, Postlethwaite, J, Pearce, J.D, Leach, G, Lye, G.J, Shamlou, P.A

“…New experimental data are reported that demonstrate the use of a novel vibrating membrane filter (VMF) for the combined recovery and concentration of two…”
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Scaleable downstream recovery of nematodes used as biopesticides by Wilson, J.A., Pearce, J.D., Shamlou, P. Ayazi

“…This study assesses the suitability of sieving as a scaleable technique for the separation of adult nematodes from infective juveniles, the latter is an…”
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A physical model of high-pressure disruption of bakers' yeast cells by Ayazi Shamlou, P., Siddiqi, S.F., Titchener-Hooker, N.J.

“…A model is presented for the disruption of microbial cells in a high-pressure homogeniser. It assumes that cell deformation and breakage due to elongational…”
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Simulation of particle size distribution changes occurring during high-pressure disruption of bakers' yeast by Siddiqi, S. F., Titchener-Hooker, N. J., Shamlou, P. Ayazi

“…Measurements of size distributions are provided for the breakage of commercial packed bakers' yeast cells as a function of operating pressure and number of…”
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Turbulent breakage of filamentous bacteria in mechanically agitated batch culture by Heydarian, S.M, Ison, A.P, Lilly, M.D, Ayazi Shamlou, P.A

“…Turbulent breakage of the S. erythraea filamentous actinomycete has been studied using a 7 L laboratory scale and a 450 l pilot scale mechanically stirred…”
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Characterization of flow intensity in continuous centrifuges for the development of laboratory mimics by Boychyn, M, Yim, S.S.S, Ayazi Shamlou, P, Bulmer, M, More, J, Hoare, M

“…Predicting the recovery of “delicate” biological materials by centrifugation using laboratory centrifuges has been a major challenge to biochemical engineers…”
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The influence of mechanical forces on the morphology and penicillin production of Penicillium chrysogenum by Makagiansar, H. Y., Ayazi Shamlou, P., Thomas, C. R., Lilly, M. D.

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Saccharomyces cerevisiae fermentations in a pilot scale airlift bioreactor: Comparison of air sparger configurations by Pollard, D. J., Ayazi Shamlou, P., Lilly, M. D., Ison, A. P.

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Gas holdup and liquid velocity in airlift bioreactors containing viscous newtonian liquids by Rajarajan, J., Pollard, D., Ison, A. P., Shamlou, P. Ayazi

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Quantitation of supercoiled circular content in plasmid DNA solutions using a fluorescence-based method by Levy, M S, Lotfian, P, O'Kennedy, R, Lo-Yim, M Y, Shamlou, P A

“…A method for quantifying the proportion of supercoiled circular (SC) forms in DNA solutions is described. The method (SCFluo) takes advantage of the reversible…”
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Ultra scaledown to predict filtering centrifugation of secreted antibody fragments from fungal broth by Boulding, N., Yim, S. S. S., Keshavarz-Moore, E., Ayazi Shamlou, P., Berry, M.

“…Extracellularly expressed anti–hen egg lysozyme single‐chain antibody fragments (scFv) produced by Aspergillus awamori were recovered using filtering…”
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Removal of contaminant nucleic acids by nitrocellulose filtration during pharmaceutical-grade plasmid DNA processing by Levy, M.S, Collins, I.J, Tsai, J.T, Ayazi Shamlou, P, Ward, J.M, Dunnill, P

“…Pharmaceutical-grade plasmid DNA for use in vaccines and gene therapy requires the development of reproducible and scaleable downstream processes. Shearing of…”
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