Dogs cloned from adult somatic cells

Dogs cloned from adult somatic cells

Several mammals - including sheep, mice, cows, goats, pigs, rabbits, cats, a mule, a horse and a litter of three rats - have been cloned by transfer of a nucleus from a somatic cell into an egg cell (oocyte) that has had its nucleus removed. This technology has not so far been successful in dogs bec...

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Bibliographic Details
Published in:Nature Vol. 436; no. 7051; p. 641
Main Authors: Lee, B.C, Kim, M.K, Jang, G, Oh, H.J, Yuda, F, Kim, H.J, Shamim, M.H, Kim, J.J, Kang, S.K, Schatten, G
Format: Journal Article
Language:English
Published: England Nature Publishing Group 04-08-2005
Subjects:
Aging
Animal breeding
Animals
cells
Cloning
cloning (animals)
Cloning, Organism
dogs
Dogs - classification
Dogs - embryology
Dogs - genetics
Dogs - growth & development
Embryo Transfer
Embryos
Female
Fibroblasts - cytology
Male
Microsatellite Repeats - genetics
nuclear transfer
Nuclear Transfer Techniques
nuclear transplantation
Oocyte Donation
Oocytes
Oocytes - cytology
Oocytes - physiology
Oocytes - transplantation
Pregnancy
Q1
skin
Skin - cytology
somatic cell nuclear transfer
Somatic cells
somatic skin cells
Stem cells
Time Factors
United States > US
Pittsburgh Pennsylvania
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Summary:Several mammals - including sheep, mice, cows, goats, pigs, rabbits, cats, a mule, a horse and a litter of three rats - have been cloned by transfer of a nucleus from a somatic cell into an egg cell (oocyte) that has had its nucleus removed. This technology has not so far been successful in dogs because of the difficulty of maturing canine oocytes in vitro. Here we describe the cloning of two Afghan hounds by nuclear transfer from adult skin cells into oocytes that had matured in vivo. Together with detailed sequence information generated by the canine-genome project, the ability to clone dogs by somatic-cell nuclear transfer should help to determine genetic and environmental contributions to the diverse biological and behavioural traits associated with the many different canine breeds.
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ISSN:0028-0836
1476-4687
DOI:10.1038/436641a