Development of a candidate influenza vaccine based on virus-like particles displaying influenza M2e peptide into the immunodominant loop region of hepatitis B core antigen: Insertion of multiple copies of M2e increases immunogenicity and protective efficiency

Highlights • HBc particles carrying the M2e peptide of influenza A virus are promising vaccines. • Particles carrying 1, 2 and 4 copies of M2e into the MIR of HBc were obtained. • Particles carrying 4 copies of M2e are the most immunogenic. • Protective capabilities of M2e–HBc particles correlate wi...

Full description

Saved in:

Bibliographic Details
Published in:	Vaccine Vol. 33; no. 29; pp. 3392 - 3397
Main Authors:	Ravin, Nikolai V, Blokhina, Elena A, Kuprianov, Victor V, Stepanova, Liudmila A, Shaldjan, Aram A, Kovaleva, Anna A, Tsybalova, Liudmila M, Skryabin, Konstantin G
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier India Pvt Ltd 26-06-2015 Elsevier Limited
Subjects:	Allergy and Immunology Animals Antigens Avian flu Core protein Disease Models, Animal E coli Efficiency Epitopes - genetics Epitopes - immunology Escherichia coli - genetics Female Gene Expression HBc particle Hepatitis Hepatitis B Hepatitis B core antigen Hepatitis B Core Antigens - genetics Homogeneity Immune response Immune system Immunogenicity Influenza Influenza A Influenza Vaccines - administration & dosage Influenza Vaccines - genetics Influenza Vaccines - immunology Influenza virus M2e Mice, Inbred BALB C Mutagenesis, Insertional Orthomyxoviridae Infections - prevention & control Peptides Protein folding Proteins Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - immunology Strains (organisms) Survival Analysis Vaccine Vaccines Vaccines, Synthetic - administration & dosage Vaccines, Synthetic - genetics Vaccines, Synthetic - immunology Vaccines, Virus-Like Particle - administration & dosage Vaccines, Virus-Like Particle - genetics Vaccines, Virus-Like Particle - immunology Viral infections Viral Matrix Proteins - genetics Viral Matrix Proteins - immunology Virus-like particles Viruses HBc particle M2e Vaccine Influenza virus
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Highlights • HBc particles carrying the M2e peptide of influenza A virus are promising vaccines. • Particles carrying 1, 2 and 4 copies of M2e into the MIR of HBc were obtained. • Particles carrying 4 copies of M2e are the most immunogenic. • Protective capabilities of M2e–HBc particles correlate with M2e copy number. • M2e of different influenza strains may be presented simultaneously on HBc particles.
ISSN:	0264-410X 1873-2518
DOI:	10.1016/j.vaccine.2015.04.066