Whole-Genome Sequencing of Lactobacillus helveticus D75 and D76 Confirms Safety and Probiotic Potential

Whole-Genome Sequencing of Lactobacillus helveticus D75 and D76 Confirms Safety and Probiotic Potential

Whole-genome DNA sequencing of D75 and D76 strains (Vitaflor, Russia) was determined using the PacBio RS II platform, which was followed by de novo assembly with SMRT Portal 2.3.0. The average nucleotide identity (ANI) test showed that both strains belong to the , but not to the , as previously assu...

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Bibliographic Details
Published in:Microorganisms (Basel) Vol. 8; no. 3; p. 329
Main Authors: Toropov, Vyacheslav, Demyanova, Elena, Shalaeva, Olga, Sitkin, Stanislav, Vakhitov, Timur
Format: Journal Article
Language:English
Published: Switzerland MDPI 26-02-2020
MDPI AG
Subjects:
bacteriocins
gene expression
lactobacillus helveticus
pacbio
probiotic genes
probiotics
whole genome sequencing
whole genome sequencing
Lactobacillus helveticus
probiotic genes
bacteriocins
PacBio
gene expression
probiotics
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Summary:Whole-genome DNA sequencing of D75 and D76 strains (Vitaflor, Russia) was determined using the PacBio RS II platform, which was followed by de novo assembly with SMRT Portal 2.3.0. The average nucleotide identity (ANI) test showed that both strains belong to the , but not to the , as previously assumed. In addition, 31 exopolysaccharide (EPS) production genes (nine of which form a single genetic cluster), 13 adhesion genes, 38 milk protein and 11 milk sugar utilization genes, 13 genes for and against specific antagonistic activity, eight antibiotic resistance genes, and also three CRISPR blocks and eight Cas I-B system genes were identified in the genomes of both strains. The expression of bacteriocin helveticin J genes was confirmed. In fact, the presence of identified genes suggests that D75 and D76 are able to form biofilms on the outer mucin layer, inhibit the growth of pathogens and pathobionts, utilize milk substrates with the formation of digestible milk sugars and bioactive peptides, resist bacteriophages, show some genome-determined resistance to antibiotics, and stimulate the host's immune system. Pathogenicity genes have not been identified. The study results confirm the safety and high probiotic potential of the strains.
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ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms8030329