Phytochemical screening, antimicrobial activity, in vitro and in vivo antioxidant activity of Berberis lycium Royle root bark extract/Triagem fitoquimica, atividade antimicrobiana, atividade antioxidante in vitro e in vivo do extrato de casca de raiz de Berberis lycium Royle

Phytochemical screening, antimicrobial activity, in vitro and in vivo antioxidant activity of Berberis lycium Royle root bark extract/Triagem fitoquimica, atividade antimicrobiana, atividade antioxidante in vitro e in vivo do extrato de casca de raiz de Berberis lycium Royle

Antioxidants are materials that scavenge or remove free radicals from living systems. The oxidation process ends in the production of free radicals. These free radicals are the chief birthplace of cancerous cells. Antioxidizing agents remove free radical intermediates by terminating oxidation proces...

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Bibliographic Details
Published in:Brazilian journal of biology Vol. 84
Main Authors: Mughal, T.A, Ali, S, Hassan, A, Kazmi, S.A.R, Saleem, M.Z, Shakir, H.A, Nazer, S, Farooq, M.A, Awan, M.Z, Khan, M.A, Andleeb, S, Mumtaz, S, Tahir, H.M, Gulzar, N
Format: Journal Article
Language:English
Published: Association of the Brazilian Journal of Biology 01-01-2024
Subjects:
Antibacterial agents
Antioxidants
Communicable diseases
Free radicals (Chemistry)
Medicine, Botanic
Medicine, Herbal
Pakistan
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Summary:Antioxidants are materials that scavenge or remove free radicals from living systems. The oxidation process ends in the production of free radicals. These free radicals are the chief birthplace of cancerous cells. Antioxidizing agents remove free radical intermediates by terminating oxidation processes by being oxidized themselves. On the other hand, infectious diseases affect the world on a large scale. To fight these diseases several synthetic compounds have been used. Plant based medications play important role in this regard. So, the current research aimed to investigate the antibacterial and antioxidant effect of Berberis lycium Royle root bark (BLR) extract. Berberis lycium Royle was used for phytochemical analysis and also as antimicrobial and antioxidant agents. The antimicrobial activity was evaluated by the agar well diffusion method. Current study revealed that BLR was rich in phytochemicals and toxic against tested pathogenic bacteria. BLR showed the highest activity against S. pyogenes (13.3 [+ or -] 0.8 mm). The lowest antibacterial activity was reported against E. coli (0 [+ or -] 0 mm). In case of minimum inhibitory concentration, it was observed that BLR with 10 [micro]g/mL concentration showed the highest activity while 2.5 [micro]g/mL of BLR showed the least inhibitory activity. The highest In vitro antioxidant activity was recorded as 65% at 100 [micro]g/mL. In case of in vivo antioxidant activity level of CAT, GSH and SOD were decreased while that of MDA was enhanced in groups treated with C[Cl.sub.4] as compared to the control group. BLR extract treatment reversed all these changes significantly. Current results indicate that BLR is effective against bacterial pathogens and also has antioxidant potential.
ISSN:1519-6984
DOI:10.1590/1519-6984.249742