Minicell‐based fungal RNAi delivery for sustainable crop protection

Spray‐induced gene silencing (SIGS) using topical dsRNA applications has risen as a promising, target‐specific, and environmentally‐friendly disease management strategy against phytopathogenic fungi. We demonstrated that minicell‐encapsulated dsRNA (ME‐dsRNA) halted gray mold disease progression in...

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Published in:	Microbial biotechnology Vol. 14; no. 4; pp. 1847 - 1856
Main Authors:	Islam, Md Tabibul, Davis, Zachery, Chen, Lisa, Englaender, Jacob, Zomorodi, Sepehr, Frank, Joseph, Bartlett, Kira, Somers, Elisabeth, Carballo, Sergio M., Kester, Mark, Shakeel, Ameer, Pourtaheri, Payam, Sherif, Sherif M.
Format:	Journal Article
Language:	English
Published:	United States John Wiley & Sons, Inc 01-07-2021 John Wiley and Sons Inc Wiley
Subjects:	Botrytis Brief Report Brief Reports Cell cycle Crop Protection Disease Double-stranded RNA E coli Fungi Gene silencing Genes Heredity Host plants Mold Pathogens Plant Diseases - prevention & control Plant protection Ribonuclease Ribonucleic acid RNA RNA Interference RNA-mediated interference siRNA Sustainable agriculture Virulence
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Summary:	Spray‐induced gene silencing (SIGS) using topical dsRNA applications has risen as a promising, target‐specific, and environmentally‐friendly disease management strategy against phytopathogenic fungi. We demonstrated that minicell‐encapsulated dsRNA (ME‐dsRNA) halted gray mold disease progression in strawberries. ME‐dsRNAs offer a platform that can readily be translated to large‐scale production and deployed in open‐field applications to control gray mold in strawberries. Summary Spray‐induced gene silencing (SIGS) using topical dsRNA applications has risen as a promising, target‐specific, and environmentally friendly disease management strategy against phytopathogenic fungi. However, dsRNA stability, efficacy, and scalability are still the main constraints facing SIGS broader application. Here we show that Escherichia coli‐derived anucleated minicells can be utilized as a cost‐effective, scalable platform for dsRNA production and encapsulation. We demonstrated that minicell‐encapsulated dsRNA (ME‐dsRNA) was shielded from RNase degradation and stabilized on strawberry surfaces, allowing dsRNA persistence in field‐like conditions. ME‐dsRNAs targeting chitin synthase class III (Chs3a, Chs3b) and DICER‐like proteins (DCL1 and DCL2) genes of Botryotinia fuckeliana selectively knocked‐down the target genes and led to significant fungal growth inhibition in vitro. We also observed a compensatory relationship between DCL1 and DCL2 gene transcripts, where the silencing of one gene upregulated the expression of the other. Contrary to naked‐dsRNAs, ME‐dsRNAs halted disease progression in strawberries for 12 days under greenhouse conditions. These results elucidate the potential of ME‐dsRNAs to enable the commercial application of RNAi‐based, species‐specific biocontrols comparable in efficacy to conventional synthetics. ME‐dsRNAs offer a platform that can readily be translated to large‐scale production and deployed in open‐field applications to control grey mould in strawberries.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Funding information The authors are grateful for the funding from The Virginia Catalyst (Fund # 460380).
ISSN:	1751-7915 1751-7915
DOI:	10.1111/1751-7915.13699