Microbiological profile of blood stream infection: Genetic relatedness of carbapenemase producing klebsiella Pneumoniae isolates using ERIC PCR

Microbiological profile of blood stream infection: Genetic relatedness of carbapenemase producing klebsiella Pneumoniae isolates using ERIC PCR

Background: There has been an expansion in antibiotic resistance among Enterobacteriaceae over the previous ten years raising serious questions about global health. Our objectives were to isolate the carbapenem-resistant Gram-negative bacilli that cause blood stream infections and investigate the ge...

Full description

Saved in:
Bibliographic Details
Published in:Microbes and Infectious Diseases Vol. 4; no. 1; pp. 168 - 181
Main Authors: Marwa Taha, Sarah Shouib, Walid ELmonir, Shaima Zahra, Eman Hegazy
Format: Journal Article
Language:English
Published: Zagazig University, Faculty of Medicine 01-02-2023
Subjects:
bsis
carbapenem resistant gram-negative bacilli
eric pcr
k. pneumoniae
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background: There has been an expansion in antibiotic resistance among Enterobacteriaceae over the previous ten years raising serious questions about global health. Our objectives were to isolate the carbapenem-resistant Gram-negative bacilli that cause blood stream infections and investigate the genetic relatedness between carbapenemase producingKlebsiella pneumoniae (K. pneumoniae) isolates. Methods: A total of 255 blood specimens were withdrawn into blood culture bottles and incubated, pathogenic microorganisms were identified, antibiotic susceptibility was done for Gram-negative isolates. Carbapenemase producers were screened for molecular detection of carbapenemase and extended spectrum β-lactamase genes. Results: One hundred and thirty-three (133) pathogens were isolated, of these 77 Gram-negative isolates (including 32 K. pneumoniae,18 E. coli, 12 Acinetobacter, 9 Pseudomonas and 6 Enterobacter cloacae). Out of them, 55 were carbapenem resistant by phenotypic method. 23/55 of them were harboring carbapenemase genes by PCR ERIC, results showed that Anesthesia intensive care unit (ICU) isolates showed >90% genetic similarity while isolates from Chest and Internal medicine ICUs showed <70% similarity. Conclusion: Increased awareness, persistent observation, and strict adherence to the recommendations for antibiotic stewardship should be used to limit spread of carbapenem resistant Gram-negative bacilli.
ISSN:2682-4132
2682-4140
DOI:10.21608/mid.2022.175107.1414