Association of vitamin D receptor gene BsmI (A>G) and FokI (C>T) polymorphism in gestational diabetes among Saudi Women

Vitamin D receptor (VDR) gene polymorphism have a role in diabetes mellitus pathogenesis. Present study was conducted to determine VDR gene variants among Saudi gestational diabetics (GDM) in Madina, KSA. This cross sectional study was conducted on 112 GDM patients and 218 normal healthy control. Ag...

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Bibliographic Details
Published in:	Pakistan journal of medical sciences Vol. 31; no. 6; pp. 1328 - 1333
Main Authors:	El-Beshbishy, Hesham A, Tawfeek, Manal A, Taha, Inass M, FadulElahi, Thoraya, Shaheen, Amal Y, Bardi, Fouad A, Sultan, Intessar I
Format:	Journal Article
Language:	English
Published:	Pakistan Knowledge Bylanes 01-11-2015 AsiaNet Pakistan (Pvt) Ltd Professional Medical Publications
Subjects:	Original Saudi Arabia BsmI Gestational diabetes FokI Vitamin D receptor Polymorphism
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Summary:	Vitamin D receptor (VDR) gene polymorphism have a role in diabetes mellitus pathogenesis. Present study was conducted to determine VDR gene variants among Saudi gestational diabetics (GDM) in Madina, KSA. This cross sectional study was conducted on 112 GDM patients and 218 normal healthy control. Age, body mass index and blood pressure levels were recorded. Serum triglycerides (mg/dl), total cholesterol, HDL-cholesterol, LDL-cholesterol, fasting blood glucose FBG and post-prandial blood glucose PPBG were estimated. Extracted DNA template was amplified by PCR reaction and genotyped for single nucleotide polymorphism of BsmI and FokI by restriction fragment length polymorphism-PCR (RFLP-PCR) analysis. FBG and PPBG levels in GDM patients were significantly elevated by +48.6% and +50%, respectively (P=0.005). Serum triglycerides, total cholesterol and LDL-cholesterol (mg/dl) levels in GDM patients were elevated significantly by +40.5% (P=0.005), +16% (P=0.01) and +30.8% (P=0.005), respectively. Serum HDL-cholesterol (mg/dl) showed significant decline by -10.5%. FokI VDR genotypes showed association with PPBG (P=0.05) among GDM patients. The Ff, FF and ff genotype percentage among GDM patients was 48.2%, 30.4% and 21.4%, respectively. FokI (F and f) and BsmI (B and b) alleles frequency showed no significant difference between GDM patients and control. Percentage BsmI and FokI total homozygous and heterozygous variants among GDM was 45.5% and 81.4%, respectively. VDR BsmI and FokI polymorphic marker not associated with Saudi GDM.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1682-024X 1681-715X
DOI:	10.12669/pjms.316.7525