Expression levels of pro-inflammatory interleukin-8 and certain antimicrobial peptides in concurrent with bacterial conjunctivitis

To detect the quantitative expression levels of the pro-inflammatory interleukin-8 (IL8), antimicrobial peptides human beta defense-2 (HBD2), and human beta defense-3 (HBD3) genes in bacterial conjunctivitis. The human conjunctival epithelial cells were obtained using the impression cytology techniq...

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Bibliographic Details
Published in:	International journal of ophthalmology Vol. 14; no. 5; pp. 666 - 675
Main Authors:	Hosny, Alaa El-Dien Shawky, El-Bazza, Zeinab El-Demerdash, Ramadan, Mohammed Abdelhalim, Shafik, Maha Ahmed, Shafeek, Mahmoud Ahmed, Khattab, Rania Abdelmonem
Format:	Journal Article
Language:	English
Published:	China International Journal of Ophthalmology Press 18-05-2021 Press of International Journal of Ophthalmology (IJO PRESS)
Subjects:	Clinical Research human beta defense-2 human beta defense-3 infectious conjunctivitis interleukin-8 real-time polymerase chain reaction human beta defense-3 human beta defense-2 interleukin-8 infectious conjunctivitis real-time polymerase chain reaction
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Summary:	To detect the quantitative expression levels of the pro-inflammatory interleukin-8 (IL8), antimicrobial peptides human beta defense-2 (HBD2), and human beta defense-3 (HBD3) genes in bacterial conjunctivitis. The human conjunctival epithelial cells were obtained using the impression cytology technique from healthy controls and patients. The genes expression levels were determined utilizing a reverse transcription quantitative polymerase chain reaction (RT-qPCR). The contribution of causative agent type, the number of isolates and severity of clinical features, in the increase of genes expression was also determined. The RT-qPCR showed that IL8, HBD2, and HBD3 expression increased in bacterial conjunctivitis as compared to healthy control ( <0.001). In gram-negative bacterial conjunctivitis, HBD2 was highly up-regulated ( <0.001) compared to other types of bacterial conjunctivitis. In mixed bacterial conjunctivitis, a direct correlation between HBD2 up-regulation and HBD3 up-regulation was observed ( <0.05). The severity of clinical features was related to the up-regulation of IL8 and HBD2 ( <0.05). IL8, HBD2, and HBD3 are immune-effectors in infectious conjunctivitis. HBD2 is active during different bacterial conjunctivitis but is more released with gram-negative bacteria compared to gram-positive bacteria. HBD3 is an obvious defender in different bacterial conjunctivitis.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2222-3959 2227-4898
DOI:	10.18240/ijo.2021.05.05