Donor-matched comparison of dental pulp stem cells and bone marrow-derived mesenchymal stem cells in a rat model

Dental pulp stem cells (DPSCs) have drawn much interest for the regeneration of mineralized tissues, and several studies have compared DPSCs to bone marrow‐derived mesenchymal stem cells (BMMSCs). However, conflicting results, possibly due to donor‐associated variability, have been published and the...

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Bibliographic Details
Published in:	Journal of tissue engineering and regenerative medicine Vol. 4; no. 1; pp. 73 - 81
Main Authors:	Alge, Daniel L., Zhou, Dan, Adams, Lyndsey L., Wyss, Brandon K., Shadday, Matthew D., Woods, Erik J., Gabriel Chu, T. M., Goebel, W. Scott
Format:	Journal Article
Language:	English
Published:	Chichester, UK John Wiley & Sons, Ltd 01-01-2010
Subjects:	Adult Stem Cells - cytology Adult Stem Cells - metabolism Animals Base Sequence bone marrow Bone Marrow Cells - cytology Bone Marrow Cells - metabolism Bone Regeneration Cell Differentiation Cell Proliferation clonogenicity colony formation Colony-Forming Units Assay Dental Pulp - cytology dental pulp stem cells differentiation DNA Primers - genetics donor variation Flow Cytometry mesenchymal stem cells Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - metabolism Models, Animal proliferation Rats Rats, Sprague-Dawley Tissue Donors Tissue Engineering - methods
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Summary:	Dental pulp stem cells (DPSCs) have drawn much interest for the regeneration of mineralized tissues, and several studies have compared DPSCs to bone marrow‐derived mesenchymal stem cells (BMMSCs). However, conflicting results, possibly due to donor‐associated variability, have been published and the regenerative potential of DPSCs is currently unclear. In the present study we have sought to address this problem using a donor‐matched experimental design to robustly compare the biological properties of DPSCs and BMMSCs. All experiments were performed using cells isolated from a single adult Sprague–Dawley rat. Our results show that DPSCs and BMMSCs had similar morphologies and flow cytometry profiles, were capable of forming colonies in vitro and were capable of osteogenic, chondrogenic and adipogenic differentiation. However, quantitative comparisons revealed that DPSCs had a faster population doubling time and a higher percentage of stem/progenitor cells in the population, as determined by clonogenic assays. Furthermore, while both cell populations formed mineral in vitro, DPSCs had significantly higher alkaline phosphatase activity than BMMSCs after 3 weeks in osteogenic medium. These data show several key differences between DPSCs and BMMSCs and support the possibility of using DPSCs for mineralized tissue regeneration. Copyright © 2009 John Wiley & Sons, Ltd.
Bibliography:	istex:1782FEC919736CAC60ADD9C6BF12EA82002EE974 ark:/67375/WNG-W1WWFPGK-5 National Institutes of Health - No. K08 HL75253; No. 1R43RR023962 General BioTechnology LLC ArticleID:TERM220 Riley Children's Foundation ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1932-6254 1932-7005
DOI:	10.1002/term.220