Search Results - "Shackman, Jonathan G"

Rapid, direct, and sensitive determination of aziridine and 2-chloroethylamine by hydrophilic interaction liquid chromatography-mass spectrometry by Shackman, Jonathan G.

“…[Display omitted] A rapid HILIC-MS method was developed for measuring the genotoxic impurities aziridine and 2-chloroethylamine. Sample preparation was simple…”
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Microfluidic Chip for Continuous Monitoring of Hormone Secretion from Live Cells Using an Electrophoresis-Based Immunoassay by Roper, Michael G, Shackman, Jonathan G, Dahlgren, Gabriella M, Kennedy, Robert T

“…A microfluidic device has been developed for the determination of insulin secreted from islets of Langerhans by a capillary electrophoresis competitive…”
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Gradient Elution Moving Boundary Electrophoresis for High-Throughput Multiplexed Microfluidic Devices by Shackman, Jonathan G, Munson, Matthew S, Ross, David

“…A novel method for performing electrophoretic separations is describedgradient elution moving boundary electrophoresis (GEMBE). The technique utilizes the…”
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Perfusion and chemical monitoring of living cells on a microfluidic chip by Shackman, Jonathan G, Dahlgren, Gabriella M, Peters, Jennifer L, Kennedy, Robert T

“…A microfluidic device that incorporates continuous perfusion and an on-line electrophoresis immunoassay was developed, characterized, and applied to monitoring…”
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Microfluidic Electrophoresis Chip Coupled to Microdialysis for in Vivo Monitoring of Amino Acid Neurotransmitters by Sandlin, Zechariah D, Shou, Minshan, Shackman, Jonathan G, Kennedy, Robert T

“…Microfluidic electrophoresis devices were coupled on-line to microdialysis for in vivo monitoring of primary amine neurotransmitters in rat brain. The devices…”
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Trapping mode two-dimensional liquid chromatography for quantitative low-level impurity enrichment in pharmaceutical development by Lin, Ziqing, Wang, Qinggang, Zhou, Yiyang, Shackman, Jonathan G.

“…•Simple and robust trapping mode 2D-LC for trace pharmaceutical impurity analysis.•Quantitative enrichment via multiple trappings demonstrated with variety of…”
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Capillary and Microfluidic Gradient Elution Isotachophoresis Coupled to Capillary Zone Electrophoresis for Femtomolar Amino Acid Detection Limits by Davis, Nejea I, Mamunooru, Manasa, Vyas, Chandni A, Shackman, Jonathan G

“…In this work, gradient elution isotachophoresis was combined with capillary zone electrophoresis (GEITP-CZE) in a single microcolumn. The multistage approach…”
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A simulation-guided approach to the selection of RPLC columns for platform small molecule separations by Liu, Zhiyang, Foley, Joe P., Shackman, Jonathan G., Wang, Qinggang, Zhou, Yiyang

“…•Column selection was explored via simulations based on the HSM column database.•10,000 virtual samples with 8, 10, 12, 14, or 16 components were used at pH…”
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Negative mode sheathless capillary electrophoresis electrospray ionization-mass spectrometry for metabolite analysis of prokaryotes by Edwards, James L., Chisolm, Claire N., Shackman, Jonathan G., Kennedy, Robert T.

“…Capillary electrophoresis (CE) was coupled to negative mode electrospray ionisation-mass spectrometry (MS) for separation and detection of phosphorylated and…”
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Discrepancies in column parameters presented in hydrophobic subtraction model manuscripts by Shackman, Jonathan G.

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Development of tandem-column liquid chromatographic methods for pharmaceutical compounds using simulations based on hydrophobic subtraction model parameters by Liu, Zhiyang, Zhou, Yiyang, Wang, Qinggang, Foley, Joe P., Stoll, Dwight R., Shackman, Jonathan G.

“…•Developed a workflow to prioritize single and tandem-column pairs for RPLC separations.•Separation predictions based upon hydrophobic subtraction model (HSM)…”
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Measuring atropisomers of BMS-986142 using 2DLC as an enabling technology by Wang, Qinggang, He, Brian Lingfeng, Shackman, Jonathan G.

“…[Display omitted] •2DLC was applied to impurity profiling of BMS-986142.•2DLC allowed for targeted profiling of only atropisomers.•Multiple-heart cutting…”
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Improved hydrophobic subtraction model of reversed-phase liquid chromatography selectivity based on a large dataset with a focus on isomer selectivity by Rutan, Sarah C., Kempen, Trevor, Dahlseid, Tina, Kruger, Zachary, Pirok, Bob, Shackman, Jonathan G., Zhou, Yiyang, Wang, Qinggang, Stoll, Dwight R.

“…•A data-driven model for reversed phase selectivity is established.•A revised hydrophobic subtraction model is proposed.•Accurate predictions of isomer…”
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Tunable normal phase enantioselectivity of amino acid esters via mobile phase composition by Yang, Alice, Shackman, Jonathan G., Ye, Yun K.

“…•Ethanesulfonic acid effect on chiral separation of amino acid esters was studied.•Using a bonded chiral phase increased bulk solvent flexibility.•ESA…”
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P(III) vs P(V): A P(V) Reagent for Thiophosphoramidate Linkages and Application to an Asymmetric Synthesis of a Cyclic Dinucleotide STING Agonist by Zheng, Bin, Hang, Chao, Zhu, Jason, Purdum, Geoffrey E, Sezen-Edmonds, Melda, Treitler, Daniel S, Yu, Miao, Yuan, Changxia, Zhu, Ye, Freitag, Adam, Guo, Siwei, Zhu, Guanghui, Hritzko, Ben, Paulson, James, Shackman, Jonathan G, He, Brian L, Fu, Weiqing, Tai, Hua Chia, Ayers, Sloan, Park, Hyunsoo, Eastgate, Martin D, Cohen, Ben, Rogers, Amanda, Wang, Qinggang, Schmidt, Michael A

“…A highly stereoselective synthesis of a cyclic dinucleotide (CDN) STING agonist containing two chiral thiophos­phoramidate linkages is described. These rare…”
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Counter-flow gradient electrofocusing by Shackman, Jonathan G., Ross, David

“…Counter‐flow gradient electrofocusing techniques are methods whereby a combination of electrophoresis and a bulk solution counter‐flow is used to accumulate or…”
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Leveraging AQbD Principles for Development of Challenging Drug Substance Stability-Indicating Methods by Yuill, Elizabeth M, Ileka, Kevin M, La Cruz, Thomas E, Li, Jieming, Shackman, Jonathan G, Tattersall, Peter I, Zang, Jia

“…Drug substance stability-indicating methods (SIMs) must undergo a litany of optimization and robustness stresses to ensure that the methods can be successfully…”
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High-molecular weight impurity screening by size-exclusion chromatography on a reversed-phase column by Lin, Ziqing, Ye, Yun K., Ling, Melissa, Shackman, Jonathan G., Ileka, Kevin M., Raglione, Thomas V.

“…[Display omitted] •High-molecular weight impurity screening established on a single C18 column.•Nine mobile phase conditions to cover various polymeric…”
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Temperature gradient focusing for microchannel separations by Shackman, Jonathan G, Munson, Matthew S, Ross, David

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Gradient Elution Isotachophoresis for Enrichment and Separation of Biomolecules by Shackman, Jonathan G, Ross, David

“…A novel format for performing capillary isotachophoresis (ITP) is describedgradient elution ITP (GEITP). GEITP merges the recently described electrophoretic…”
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