Analysis of the biological and molecular variability of Watermelon mosaic virus isolates from Iran

Analysis of the biological and molecular variability of Watermelon mosaic virus isolates from Iran

Watermelon mosaic virus (WMV) is one of the most important viruses that causes different symptoms in Cucurbitaceae. WMV is a potyvirus with a worldwide distribution, but occurs most commonly in temperate and Mediterranean regions. Cucurbit species grown in Yazd, Esfahan, West Azerbaijan, Hormozgan,...

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Bibliographic Details
Published in:Virus genes Vol. 37; no. 3; pp. 304 - 313
Main Authors: Sharifi, Maraym, Massumi, Hossain, Heydarnejad, Jahangir, Hosseini Pour, Akbar, Shaabanian, Mehdi, Rahimian, H
Format: Journal Article
Language:English
Published: Boston Boston : Springer US 01-12-2008
Birkhäuser-Verlag
Springer Nature B.V
Subjects:
Amino acids
Aphididae
Biomedical and Life Sciences
Biomedicine
Capsid Proteins - genetics
Citrullus colocynthis
Citrullus lanatus
Cloning, Molecular
Coat protein
Cucurbitaceae
Cucurbitaceae - virology
Datura metel
Enzyme-linked immunosorbent assay
Genetic Variation
Host range
Iran
Medical Microbiology
Molecular Sequence Data
Nicotiana
Nucleotides
Phylogenetics
Phylogeny
Plant Diseases - virology
Plant Sciences
Plant virus diseases
Polyclonal antibodies
Potyvirus
Potyvirus - classification
Potyvirus - genetics
Potyvirus - isolation & purification
Sequence analysis
Sequence Analysis, DNA
Species
Virology
Viruses
Weeds
Iran
Watermelon mosaic virus
Coat protein
Phylogeny
Iran
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Summary:Watermelon mosaic virus (WMV) is one of the most important viruses that causes different symptoms in Cucurbitaceae. WMV is a potyvirus with a worldwide distribution, but occurs most commonly in temperate and Mediterranean regions. Cucurbit species grown in Yazd, Esfahan, West Azerbaijan, Hormozgan, and Kerman provinces were surveyed for the relative incidence of WMV in 2004-2005. A total of 757 symptomatic cucurbit and 31 weed species were collected and assayed for infection with WMV. Of 788 leaf samples from cucurbit and weed plants, 190 samples were positive by double antibody sandwich ELISA (DAS-ELISA) using specific polyclonal antibody. Among the weed species tested only colocynth (Citrullus colocynthis) was found to be infected with WMV. The coat protein (CP) gene from 18 representative isolates was PCR amplified, cloned, sequenced, and compared with the sequences available in GeneBank. Phylogenetic analysis using 778 nucleotide long sequences of the coat protein gene showed that these isolates fell into two; groups I and II. Only one isolates (KER.JI.1) was classified in the group II. This isolate had a wider host range and infected Nicotiana debneyii and Datura metel. None of the other 17 isolates could infect these two species. Members of group I were divided into three subgroups; A, B, and C. The subgroup IB appears to be a new subgroup comprising only of the Iranian isolates. Phylogenetic analysis based on 200 nucleotides coding for the N-terminal segment of the CP showed that all Iranian isolates except KER.JI.1 clustered with the previously reported WMV strains. All Iranian isolates had a DAG amino acid triplet which is involved in aphid transmissibility. This is the first report on sequence analysis of the nearly full-length CP cDNA clones of WMV isolates from Iran.
Bibliography:http://dx.doi.org/10.1007/s11262-008-0271-8
ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0920-8569
1572-994X
DOI:10.1007/s11262-008-0271-8