The distribution of circulating microRNA and their relation to coronary disease
MicroRNAs (miRNAs) are small RNAs that regulate gene expression by suppressing protein translation and may influence RNA expression. MicroRNAs are detected in extracellular locations such as plasma; however, the extent of miRNA expression in plasma its relation to cardiovascular disease is not clear...
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| Published in: | F1000 research Vol. 1; p. 50 |
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| Main Authors: | , , , , , , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
England
F1000Research
2012
F1000 Research Ltd |
| Subjects: | |
| Online Access: | Get full text |
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| Summary: | MicroRNAs (miRNAs) are small RNAs that regulate gene expression by suppressing protein translation and may influence RNA expression. MicroRNAs are detected in extracellular locations such as plasma; however, the extent of miRNA expression in plasma its relation to cardiovascular disease is not clear and many clinical studies have utilized array-based platforms with poor reproducibility.
Initially, to define distribution of miRNA in human blood; whole blood, platelets, mononuclear cells, plasma, and serum from 5 normal individuals were screened for 852 miRNAs using high-throughput micro-fluidic quantitative RT-PCR (qRT-PCR). In total; 609, 448, 658, 147, and 178 miRNAs were found to be expressed in moderate to high levels in whole blood, platelets, mononuclear cells, plasma, and serum, respectively, with some miRNAs uniquely expressed. To determine the cardiovascular relevance of blood miRNA expression, plasma miRNA (n=852) levels were measured in 83 patients presenting for cardiac catheterization. Eight plasma miRNAs were found to have over 2-fold increased expression in patients with significant coronary disease (≥70% stenosis) as compared to those with minimal coronary disease (less than 70% stenosis) or normal coronary arteries. Expression of miR-494, miR-490-3p, and miR-769-3p were found to have significantly different levels of expression. Using a multivariable regression model including cardiovascular risk factors and medications, hsa-miR-769-3p was found to be significantly correlated with the presence of significant coronary atherosclerosis.
This study utilized a superior high-throughput qRT-PCR based method and found that miRNAs are found to be widely expressed in human blood with differences expressed between cellular and extracellular fractions. Importantly, specific miRNAs from circulating plasma are associated with the presence of significant coronary disease. |
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| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 All authors contributed to this work. There were no paid authors or writing assistants used in the preparation of the manuscript or analysis of the data. All authors declare that the did not submit related or duplicate manuscripts elsewhere. Jane E. Freedman, MD: design of the study and writing the manuscript. Bahadir Ercan, PhD: conducting the PCR analysis. Kristine M. Morin, MPH: analyzing the data. Ching-Ti Liu, PhD: analyzing the data. Lulufer Tamer, PhD: recruiting patient and collecting the blood samples and conducting the RNA isolations. Lokman Ayaz, MSc: conducting the RNA isolations. Mehmet Kanadasi, MD, Dilek Cicek, MD, Ali Ihsan Seyhan, MD, Rabia Eker Akilli, MD, Celalettin Camci, MD, and Beyhan Cengiz, PhD: recruiting patients and collecting the blood samples. Serdar Oztuzcu, MD: conducting the PCR analysis. Kahraman Tanriverdi, PhD: design of the study, conducting the PCR analysis and writing the manuscript. Competing interests: No competing interests were disclosed. |
| ISSN: | 2046-1402 2046-1402 |
| DOI: | 10.12688/f1000research.1-50.v1 |