Investigating the effects of adult neural stem cell transplantation by lumbar puncture in transient cerebral ischemia

► The lumbar puncture is a safe method to inject neural stem cells (NSCs). ► The NSCs can migrate from lumbar region to the subependymal zone. ► The NSCs are able to differentiate to different neuronal and glial cells. ► Transplanted NSCs improve the coordination in movement in ischemic animals. Ste...

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Bibliographic Details
Published in:	Neuroscience letters Vol. 495; no. 1; pp. 1 - 5
Main Authors:	Seyed Jafari, S.S., Ali Aghaei, A., Asadi-Shekaari, M., Nematollahi-mahani, S.N., Sheibani, V.
Format:	Journal Article
Language:	English
Published:	Shannon Elsevier Ireland Ltd 09-05-2011 Elsevier
Subjects:	Adult Stem Cells - cytology Adult Stem Cells - physiology Adult Stem Cells - transplantation Animals Biological and medical sciences Cell Differentiation Cell Movement Cell therapy Fundamental and applied biological sciences. Psychology Ischemia Ischemic Attack, Transient - pathology Ischemic Attack, Transient - physiopathology Ischemic Attack, Transient - therapy Lumbar puncture Male Medical sciences Movement Neural stem cells Neural Stem Cells - cytology Neural Stem Cells - physiology Neural Stem Cells - transplantation Neuroglia - pathology Neuroglia - physiology Neurology Neurons - pathology Neurons - physiology Rats Rats, Wistar Spinal Puncture Vascular diseases and vascular malformations of the nervous system Vertebrates: nervous system and sense organs Rats Neural stem cells Cell therapy Movement Ischemia Lumbar puncture Nervous system diseases Rat Stem cell Rodentia Central nervous system Cardiovascular disease Transplantation Cerebral disorder Encephalon Vascular disease Vertebrata Mammalia Animal Central nervous system disease Brain ischemia Cerebrovascular disease
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Summary:	► The lumbar puncture is a safe method to inject neural stem cells (NSCs). ► The NSCs can migrate from lumbar region to the subependymal zone. ► The NSCs are able to differentiate to different neuronal and glial cells. ► Transplanted NSCs improve the coordination in movement in ischemic animals. Stem cells have the ability to self renew and are therefore a good source for cell therapy following ischemia. In this study, we transplanted adult rat neural stem cells (NSCs) by lumbar puncture (LP) to investigate whether these cells can migrate and differentiate into neurons or glial cells, thereby improving functional outcome in cerebral ischemia. Transient ischemia was induced in adult rats (n=16) for 1h. Three days after the induction of ischemia, NSCs obtained from the subventricular zone of adult rats were injected into ischemic animals (n=8) by LP at the level of L6–S1. Improved recovery of the coordination of movement on the 1st, 7th, 14th, 21st and 28th days after the injury was examined by the Rotarod test and compared with non-transplanted ischemic animals (n=8). The presence of NSCs in the brain tissue of the animals was examined by immunohistofluorscence and immunohistochemical techniques. The coordination of movement in ischemic animals that received neural stem cells was improved significantly (P<0.05) compared with untreated ischemic animals. Cells labeled with PKH26 were observed in the ischemic area of brain tissue sections. The alkaline phosphatase test and immunohistochemical techniques demonstrated a gathering of NSCs in the lateral ventricle. A number of cells which expressed neuronal and astrocytic cell markers had migrated from the lateral ventricle to the subjacent brain parenchyma. NSCs injected by LP were able to migrate to the ischemic tissue and differentiate into neural-like cells. These differentiated cells may have improved the coordination in movement in the ischemic animals injected with NSCs.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	0304-3940 1872-7972
DOI:	10.1016/j.neulet.2011.02.025