Bacteriostatic and Cytotoxic Properties of Composite Material Based on ZnO Nanoparticles in PLGA Obtained by Low Temperature Method

A low-temperature technology was developed for producing a nanocomposite based on poly (lactic-co-glycolic acid) and zinc oxide nanoparticles (ZnO-NPs), synthesized by laser ablation. Nanocomposites were created containing 0.001, 0.01, and 0.1% of zinc oxide nanoparticles with rod-like morphology an...

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Bibliographic Details
Published in:	Polymers Vol. 14; no. 1; p. 49
Main Authors:	Burmistrov, Dmitriy E, Simakin, Alexander V, Smirnova, Veronika V, Uvarov, Oleg V, Ivashkin, Petr I, Kucherov, Roman N, Ivanov, Vladimir E, Bruskov, Vadim I, Sevostyanov, Mihail A, Baikin, Alexander S, Kozlov, Valery A, Rebezov, Maksim B, Semenova, Anastasia A, Lisitsyn, Andrey B, Vedunova, Maria V, Gudkov, Sergey V
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI AG 23-12-2021 MDPI
Subjects:	Ablation Additives Aqueous solutions Biocompatibility Biomarkers Calibration Clustering composite Composite materials Cytotoxicity Dopants Food packaging Glycolic acid Heat resistance Hydrogen peroxide Hydroxyl radicals Laser ablation Lasers Low temperature Metal oxides Microscopy Morphology Nanocomposites Nanomaterials Nanoparticles PLGA poly(lactic-co-glycolic acid) Polylactic acid Polymers Polyvinyl alcohol Rheology Synthesis Textiles Toxicity Zinc oxide Zinc oxides Austria Latvia United Kingdom > UK United States > US Japan Australia Germany Russia PLGA nanoparticles biocompatibility composite antibacterial poly(lactic-co-glycolic acid) cytotoxicity zinc oxide
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Summary:	A low-temperature technology was developed for producing a nanocomposite based on poly (lactic-co-glycolic acid) and zinc oxide nanoparticles (ZnO-NPs), synthesized by laser ablation. Nanocomposites were created containing 0.001, 0.01, and 0.1% of zinc oxide nanoparticles with rod-like morphology and a size of 40-70 nm. The surface of the films from the obtained nanomaterial was uniform, without significant defects. Clustering of ZnO-NPs in the PLGA matrix was noted, which increased with an increase in the concentration of the dopant in the polymer. The resulting nanomaterial was capable of generating reactive oxygen species (ROS), such as hydrogen peroxide and hydroxyl radicals. The rate of ROS generation increased with an increase in the concentration of the dopant. It was shown that the synthesized nanocomposite promotes the formation of long-lived reactive protein species, and is also the reason for the appearance of a key biomarker of oxidative stress, 8-oxoguanine, in DNA. The intensity of the process increased with an increase in the concentration of nanoparticles in the matrix. It was found that the nanocomposite exhibits significant bacteriostatic properties, the severity of which depends on the concentration of nanoparticles. In particular, on the surface of the PLGA-ZnO-NPs composite film containing 0.001% nanoparticles, the number of bacterial cells was 50% lower than that of pure PLGA. The surface of the composite is non-toxic to eukaryotic cells and does not interfere with their adhesion, growth, and division. Due to its low cytotoxicity and bacteriostatic properties, this nanocomposite can be used as coatings for packaging in the food industry, additives for textiles, and also as a material for biomedicine.
ISSN:	2073-4360 2073-4360
DOI:	10.3390/polym14010049