Molecular detection of equid herpesvirus in bronchoalveolar lavage fluid from asymptomatic horses in Southern Brazil

Molecular detection of equid herpesvirus in bronchoalveolar lavage fluid from asymptomatic horses in Southern Brazil

Molecular approaches to diagnose respiratory viruses have provided an opportunity for early and subclinical pathogen detection, particularly in samples from the upper respiratory tract. This study aimed to investigate the presence of herpesviruses, particularly equid herpesvirus (EHV)-2 and EHV-5, i...

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Published in:Veterinary World Vol. 15; no. 11; pp. 2597 - 2602
Main Authors: Finger, Mariane Angélica, Biava, Janaina Socolovski, Dornbusch, Peterson Triches, Perotta, João Henrique, Ullmann, Leila Sabrina, Serpa, Priscila Beatriz da SiIva, Kmetiuk, Louise Bach, Dos Santos, Andrea Pires, Biondo, Alexander Welker, Leutenegger, Christian, Filho, Ivan Roque de Barros
Format: Journal Article
Language:English
Published: India Veterinary World 01-11-2022
Subjects:
Bronchoalveolar lavage
bronchoalveolar lavage fluid
Endoscopy
Epidemiology
equine
Equine herpesvirus diseases
gammaherpesvirus
Health aspects
Infection
polymerase chain reaction
Respiratory tract diseases
tracheal wash
Brazil
bronchoalveolar lavage fluid
tracheal wash
endoscopy
gammaherpesvirus
polymerase chain reaction
equine
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Summary:Molecular approaches to diagnose respiratory viruses have provided an opportunity for early and subclinical pathogen detection, particularly in samples from the upper respiratory tract. This study aimed to investigate the presence of herpesviruses, particularly equid herpesvirus (EHV)-2 and EHV-5, in samples from the lower respiratory tract of healthy racehorses from Southern Brazil. Samples from the lower respiratory tract (i.e., bronchoalveolar lavage fluid [BALF]) were assessed by video endoscopy, cytological evaluation of BALF, and tracheal aspirates (TA), along with quantitative polymerase chain reaction (qPCR), to detect equine herpesvirus infection in the lower respiratory tract samples and compare corresponding cytological and endoscopic findings. At least one abnormality per horse during endoscopy examination was observed, including, but not limited to, mucous secretion in the airways and pharyngeal lymphoid hyperplasia. The presence of EHV-2 and/or EHV-5 was detected by qPCR in 3/10 animals. One horse was positive for EHV-2 alone, one for EHV-5 alone, and one for both. To the authors' knowledge, this is the first molecular detection of EHV-2 and EHV-5 in Brazilian thoroughbred horses. These findings may provide new insights into the epidemiology of EHV-2 and EHV-5 in Brazilian horses, evidencing the importance of the molecular investigation, early detection, and prevention of respiratory diseases.
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ISSN:0972-8988
2231-0916
DOI:10.14202/vetworld.2022.2597-2602