Synthesis and transfection activity of novel galactosylated polycationic lipid

Synthesis and transfection activity of novel galactosylated polycationic lipid

In this study, we synthesized a new galactosylated cationic lipid and investigated its biological activity. The structure of lipid combines both spermine residue for DNA compaction and galactose moiety for the improvement of aggregation behavior of lipoplexes. Lipid was low toxic for different mamma...

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Bibliographic Details
Published in:Bioorganic & medicinal chemistry letters Vol. 21; no. 10; pp. 2937 - 2940
Main Authors: Maslov, M.A., Medvedeva, D.A., Rapoport, D.A., Serikov, R.N., Morozova, N.G., Serebrennikova, G.A., Vlassov, V.V., Zenkova, М.A.
Format: Journal Article
Language:English
Published: England Elsevier Ltd 15-05-2011
Subjects:
Aggregation behavior
Animals
Cationic lipids
Cell Line
Cell Survival - drug effects
Compaction
Cricetinae
DNA
EGFP expression
Flow Cytometry
Galactose
Galactose - chemistry
gene expression
Gene silencing
Gene Transfer Techniques
Humans
Lipids
Lipids - chemical synthesis
Lipids - chemistry
Lipids - toxicity
Liposomes - chemistry
Mammalian cells
mammals
Microscopy, Atomic Force
Molecular Structure
nucleic acids
Oligonucleotides
Plasmids
Polyamines - chemistry
siRNA
small interfering RNA
Spermine
toxicity
Transfection
Transfection efficiency
Transgenes
siRNA
EGFP expression
Transfection efficiency
Cationic lipids
Oligonucleotides
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Description
Summary:In this study, we synthesized a new galactosylated cationic lipid and investigated its biological activity. The structure of lipid combines both spermine residue for DNA compaction and galactose moiety for the improvement of aggregation behavior of lipoplexes. Lipid was low toxic for different mammalian cells, and was able both to compact plasmid DNA and to mediate cellular accumulation of various nucleic acids (ODN, pDNA and siRNA) exhibiting biological activity (transgene expression, gene silencing).
Bibliography:http://dx.doi.org/10.1016/j.bmcl.2011.03.056
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0960-894X
1464-3405
DOI:10.1016/j.bmcl.2011.03.056