Effects of Different Crystalloid Fluids on Renal Tissue in an Experimental Model of Hemorrhagic Shock

Objective:The type of fluid that should be used in uncontrollable hemorrhages remains an area of research. This study was designed to compare the effects of resuscitation with Ringer’s lactate (RL) solution versus a normal saline (NS) solution on hemodynamics, renal tissue histopathology, coagulatio...

Full description

Saved in:

Bibliographic Details
Published in:	Turkish journal of anaesthesiology and reanimation Vol. 51; no. 5; pp. 38 - 387
Main Authors:	Saraçoğlu, Kemal Tolga, Saraçoğlu, Ayten, Yıldırım, Mehmet, Demirtaş, Cumaali, Akça, Metehan, Serdoğan, Ferda, İlyas Samet Ergün, Tetik, Şermin, Pençe, Sadrettin
Format:	Journal Article
Language:	English
Published:	Istanbul Aves Yayincilik Ltd. STI 01-10-2023 Galenos Publishing Galenos Publishing House
Subjects:	annexin a5 Apoptosis Hemorrhage Hemorrhagic shock normal saline Original ringer’s lactate rotem
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Objective:The type of fluid that should be used in uncontrollable hemorrhages remains an area of research. This study was designed to compare the effects of resuscitation with Ringer’s lactate (RL) solution versus a normal saline (NS) solution on hemodynamics, renal tissue histopathology, coagulation, and apoptosis in a rat model of hemorrhagic shock.Methods:The study employed groups designated as the control, hemorrhage, NS, and RL groups. Heart rate, mean arterial pressure, and respiratory rate were monitored. Annexin A5 values were assayed, rotational thromboelastometry analysis was performed, and excised kidney tissue samples were histopathologically analyzed.Results:Blood pressure levels were found to be significantly higher in the control group than those measured in the other groups. While the clotting time (CT) and clot formation time (CFT) in the hemorrhage group were significantly longer than those in the control and RL groups, the CT and CFT measured in the control group were significantly shorter compared to the RL group. The mean Annexin A5 level was in the hemorrhage group, which was significantly higher compared to the other groups. In the renal histopathological evaluation, the scores of proximal tubular injury, distal renal tubular injury, and interstitial renal tubular injury were found to be significantly lower in the control group compared to the other groups.Conclusion:This study demonstrated that NS or RL can be used safely to improve the hemodynamic symptoms resulting from hemorrhagic shock as a means to reduce apoptosis, and to decrease findings in favor of coagulopathy in bedside coagulation tests during the early stages of hemorrhagic shock until the time of starting a blood transfusion.
ISSN:	2667-677X 2667-6370
DOI:	10.4274/TJAR.2023.231262