Search Results - "Seraphin, B"

Positive feedback in eukaryotic gene networks: cell differentiation by graded to binary response conversion by Becskei, Attila, Séraphin, Bertrand, Serrano, Luis

“…Feedback is a ubiquitous control mechanism of gene networks. Here, we have used positive feedback to construct a synthetic eukaryotic gene switch in…”
Get full text

A generic protein purification method for protein complex characterization and proteome exploration by Séraphin, Bertrand, Rigaut, Guillaume, Shevchenko, Anna, Rutz, Berthold, Wilm, Matthias, Mann, Matthias

“…We have developed a generic procedure to purify proteins expressed at their natural level under native conditions using a novel tandem affinity purification…”
Get full text

Sm and Sm-like proteins belong to a large family: identification of proteins of the U6 as well as the U1, U2, U4 and U5 snRNPs by Seraphin, B

“…Several small nuclear RNAs (snRNAs), including the spliceosomal U1, U2. U4 and U5 snRNAs, are associated with Sm proteins. These eight small proteins form a…”
Get full text

The Tandem Affinity Purification (TAP) Method: A General Procedure of Protein Complex Purification by Puig, Oscar, Caspary, Friederike, Rigaut, Guillaume, Rutz, Berthold, Bouveret, Emmanuelle, Bragado-Nilsson, Elisabeth, Wilm, Matthias, Séraphin, Bertrand

“…Identification of components present in biological complexes requires their purification to near homogeneity. Methods of purification vary from protein to…”
Get full text

Sm-like protein complex that participates in mRNA degradation by Bouveret, E, Rigaut, G, Shevchenko, A, Wilm, M, Seraphin, B

“…In eukaryotes, seven Sm proteins bind to the U1, U2, U4 and U5 spliceosomal snRNAs while seven Smlike proteins (Lsm2p–Lsm8p) are associated with U6 snRNA…”
Get full text

The yeast POP2 gene encodes a nuclease involved in mRNA deadenylation by Daugeron, M C, Mauxion, F, Séraphin, B

“…The major mRNA degradation pathway involves deadenylation of the target molecule followed by decapping and, finally, 5'-->3' exonuclease digestion of the mRNA…”
Get full text

Identification and characterization of human Mex-3 proteins, a novel family of evolutionarily conserved RNA-binding proteins differentially localized to processing bodies by Buchet-Poyau, Karine, Courchet, Julien, Hir, Hervé Le, Séraphin, Bertrand, Scoazec, Jean-Yves, Duret, Laurent, Domon-Dell, Claire, Freund, Jean-Noël, Billaud, Marc

“…In Caenorhabditis elegans, the Mex-3 protein is a translational regulator that specifies the posterior blastomere identity in the early embryo and contributes…”
Get full text

The S. cerevisiae SET3 complex includes two histone deacetylases, Hos2 and Hst1, and is a meiotic-specific repressor of the sporulation gene program by Pijnappel, W W, Schaft, D, Roguev, A, Shevchenko, A, Tekotte, H, Wilm, M, Rigaut, G, Séraphin, B, Aasland, R, Stewart, A F

“…Set3 is one of two proteins in the yeast Saccharomyces cerevisiae that, like Drosophila Trithorax, contains both SET and PHD domains. We found that Set3 forms…”
Get full text

The apoptosis-promoting factor TIA-1 is a regulator of alternative pre-mRNA splicing by Förch, P, Puig, O, Kedersha, N, Martínez, C, Granneman, S, Séraphin, B, Anderson, P, Valcárcel, J

“…We report here that the apoptosis-promoting protein TIA-1 regulates alternative pre-mRNA splicing of the Drosophila melanogaster gene male-specific-lethal 2…”
Get full text

Strategies for the structural analysis of multi-protein complexes: Lessons from the 3D-Repertoire project by Collinet, B., Friberg, A., Brooks, M.A., van den Elzen, T., Henriot, V., Dziembowski, A., Graille, M., Durand, D., Leulliot, N., Saint André, C., Lazar, N., Sattler, M., Séraphin, B., van Tilbeurgh, H.

“…Structural studies of multi-protein complexes, whether by X-ray diffraction, scattering, NMR spectroscopy or electron microscopy, require stringent quality…”
Get full text

Sm and Sm‐like proteins assemble in two related complexes of deep evolutionary origin by Salgado‐Garrido, Josefa, Bragado‐Nilsson, Elisabeth, Kandels‐Lewis, Stefanie, Séraphin, Bertrand

“…A group of seven Sm proteins forms a complex that binds to several RNAs in metazoans. All Sm proteins contain a sequence signature, the Sm domain, also found…”
Get full text

RNA binding in an Sm core domain: X-ray structure and functional analysis of an archaeal Sm protein complex by Törö, Imre, Thore, Stéphane, Mayer, Claudine, Basquin, Jérôme, Séraphin, Bertrand, Suck, Dietrich

“…Eukaryotic Sm and Sm‐like proteins associate with RNA to form the core domain of ribonucleoprotein particles involved in pre‐mRNA splicing and other processes…”
Get full text

Interaction of the U1 snRNP with nonconserved intronic sequences affects 5' splice site selection by Puig, O, Gottschalk, A, Fabrizio, P, Séraphin, B

“…Intron definition and splice site selection occur at an early stage during assembly of the spliceosome, the complex mediating pre-mRNA splicing. Association of…”
Get full text

U1 snRNP-associated factor Luc7p affects 5' splice site selection in yeast and human by Puig, Oscar, Bragado-Nilsson, Elisabeth, Koski, Terhi, Séraphin, Bertrand

“…yLuc7p is an essential subunit of the yeast U1 snRNP and contains two putative zinc fingers. Using RNA-protein cross-linking and directed site-specific…”
Get full text

Accurate Processing of a Eukaryotic Precursor Ribosomal RNA by Ribonuclease MRP In vitro by Lygerou, Zoi, Allmang, Christine, Tollervey, David, Séraphin, Bertrand

“…Very few of the enzymes required for eukaryotic precursor ribosomal RNA (pre-rRNA) processing have been identified. Ribonuclease (RNase) MRP was characterized…”
Get full text

dual role for BBP/ScSF1 in nuclear pre-mRNA retention and splicing by Rutz, B, Seraphin, B

“…The MSL5 gene, which codes for the splicing factor BBP/ScSF1, is essential in Saccharomyces cerevisiae, yet previous analyses failed to reveal a defect in…”
Get full text

Transient interaction of BBP/ScSF1 and Mud2 with the splicing machinery affects the kinetics of spliceosome assembly by RUTZ, BERTHOLD, SÉRAPHIN, BERTRAND

“…Removal of introns from pre-mRNA is an essential step of gene expression. The splicing reaction is catalyzed in a large complex termed the spliceosome. Introns…”
Get full text

Dbp5, a DEAD-box protein required for mRNA export, is recruited to the cytoplasmic fibrils of nuclear pore complex via a conserved interaction with CAN/Nup159p by Schmitt, Christel, von Kobbe, Cayetano, Bachi, Angela, Panté, Nelly, Rodrigues, João P., Boscheron, Cécile, Rigaut, Guillaume, Wilm, Matthias, Séraphin, Bertrand, Carmo-Fonseca, Maria, Izaurralde, Elisa

“…Dbp5 is a DEAD‐box protein essential for mRNA export from the nucleus in yeast. Here we report the isolation of a cDNA encoding human Dbp5 (hDbp5) which is 46%…”
Get full text

Luc7p, a novel yeast U1 snRNP protein with a role in 5' splice site recognition by Fortes, P, Bilbao-Cortés, D, Fornerod, M, Rigaut, G, Raymond, W, Séraphin, B, Mattaj, I W

“…The characterization of a novel yeast-splicing factor, Luc7p, is presented. The LUC7 gene was identified by a mutation that causes lethality in a yeast strain…”
Get full text

The POP1 gene encodes a protein component common to the RNase MRP and RNase P ribonucleoproteins by Lygerou, Z, Mitchell, P, Petfalski, E, Séraphin, B, Tollervey, D

“…Two forms of the yeast 5.8S rRNA are generated from a large precursor by distinct processing pathways. Cleavage at site A3 is required for synthesis of the…”
Get full text