Cloning and characterization of microRNAs from porcine skeletal muscle and adipose tissue

Cloning and characterization of microRNAs from porcine skeletal muscle and adipose tissue

MicroRNAs (miRNAs) are an abundant class of small regulatory RNAs that regulate the stability and translation of cognate mRNAs. Although an increasing number of porcine miRNAs has recently been identified, the full repertoire of miRNAs in pig remains to be elucidated. To identify porcine miRNAs pote...

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Bibliographic Details
Published in:Molecular biology reports Vol. 37; no. 7; pp. 3567 - 3574
Main Authors: Cho, Ik Sang, Kim, Jung, Seo, Hye Youn, Lim, Do Hwan, Hong, Jae Sang, Park, Young Hee, Park, Dae Cheul, Hong, Ki-Chang, Whang, Kwang Youn, Lee, Young Sik
Format: Journal Article
Language:English
Published: Dordrecht Dordrecht : Springer Netherlands 01-10-2010
Springer Netherlands
Springer Nature B.V
Subjects:
adipose tissue
Adipose Tissue - metabolism
Animal Anatomy
Animal Biochemistry
Animals
Base Sequence
Biomedical and Life Sciences
Body fat
Cloning
Cloning, Molecular
Gene expression
Gene Expression Profiling
Gene Expression Regulation
Genome - genetics
Histology
Hogs
Life Sciences
MicroRNA
MicroRNAs - chemistry
MicroRNAs - genetics
MicroRNAs - metabolism
Molecular biology
Molecular Sequence Data
Morphology
Multigene Family - genetics
Muscle, Skeletal - metabolism
Musculoskeletal system
Nucleic Acid Conformation
Ribonucleic acid
RNA
RNA Precursors - chemistry
RNA Precursors - genetics
Sequence Analysis, RNA
skeletal muscle
Sus scrofa - genetics
swine
MicroRNA
Adipose tissue
Skeletal muscle
Pig
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Summary:MicroRNAs (miRNAs) are an abundant class of small regulatory RNAs that regulate the stability and translation of cognate mRNAs. Although an increasing number of porcine miRNAs has recently been identified, the full repertoire of miRNAs in pig remains to be elucidated. To identify porcine miRNAs potentially involved in myogenesis and adipogenesis, we constructed small RNA cDNA libraries from skeletal muscle and adipose tissue and identified 89 distinct miRNAs that are conserved in pig, of which 15 were new. Expression analysis of all newly identified and selected known porcine miRNAs revealed that some miRNAs were enriched in a tissue-specific manner, whereas others were expressed ubiquitously in the porcine tissues examined. Our results expand the number of known porcine miRNAs and provide useful information for further investigating the biological functions of miRNAs associated with growth and development of skeletal muscle or adipose tissue in pig.
Bibliography:http://dx.doi.org/10.1007/s11033-010-0005-6
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ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-010-0005-6