L-Lactic Acid Production by Lactobacillus rhamnosus ATCC 10863

Lactic acid has been shown to have the most promising application in biomaterials as poly(lactic acid). L. rhamnosus ATCC 10863 that produces L-lactic acid was used to perform the fermentation and molasses was used as substrate. A solution containing 27.6 g/L of sucrose (main composition of molasses...

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Bibliographic Details
Published in:	TheScientificWorld Vol. 2015; no. 2015; pp. 1 - 6
Main Authors:	Senedese, Ana Lívia Chemeli, Wolf Maciel, Maria Regina, Maciel Filho, Rubens
Format:	Journal Article
Language:	English
Published:	Cairo, Egypt Hindawi Publishing Corporation 2015 John Wiley & Sons, Inc Hindawi Limited
Subjects:	Acid production Acids Batch Cell Culture Techniques Biomaterials Cell growth Fermentation Glucose - biosynthesis Health aspects Lactic acid Lactic Acid - biosynthesis Lactobacillus Lactobacillus rhamnosus Lactobacillus rhamnosus - metabolism Liquid chromatography Sucrose Sucrose - metabolism Sugarcane Yeast Yeasts
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Summary:	Lactic acid has been shown to have the most promising application in biomaterials as poly(lactic acid). L. rhamnosus ATCC 10863 that produces L-lactic acid was used to perform the fermentation and molasses was used as substrate. A solution containing 27.6 g/L of sucrose (main composition of molasses) and 3.0 g/L of yeast extract was prepared, considering the final volume of 3,571 mL (14.0% (v/v) inoculum). Batch and fed batch fermentations were performed with temperature of 43.4°C and pH of 5.0. At the fed batch, three molasses feed were applied at 12, 24, and 36 hours. Samples were taken every two hours and the amounts of lactic acid, sucrose, glucose, and fructose were determined by HPLC. The sucrose was barely consumed at both processes; otherwise the glucose and fructose were almost entirely consumed. 16.5 g/L of lactic acid was produced at batch and 22.0 g/L at fed batch. Considering that lactic acid was produced due to the low concentration of the well consumed sugars, the final amount was considerable. The cell growth was checked and no substrate inhibition was observed. A sucrose molasses hydrolysis is suggested to better avail the molasses fermentation with this strain, surely increasing the L-lactic acid.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Academic Editor: Anton Ficai
ISSN:	2356-6140 1537-744X 1537-744X
DOI:	10.1155/2015/501029