Search Results - "Senear, D F"

Footprint'' Titrations Yield Valid Thermodynamic Isotherms by Brenowitz, Michael, Senear, Donald F., Shea, Madeline A., Ackers, Gary K.

“…A central issue in gene regulation is the mechanism, and biological function, of the cooperative binding of regulatory protein ligands to specific sites on…”
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Spectral Enhancement of Proteins: Biological Incorporation and Fluorescence Characterization of 5-Hydroxytryptophan in Bacteriophage λ cI Repressor by J. B. Alexander Ross, Senear, Donald F., Waxman, Evan, Kombo, Bamenga B., Rusinova, Elena, Huang, Yao Te, Laws, William R., Hasselbacher, C. A.

“…We have used a tryptophan-requiring Escherichia coli auxotroph to replace the three tryptophan residues of λ cI repressor with 5-hydroxy-L-tryptophan…”
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Determination of binding constants for cooperative site-specific protein-DNA interactions using the gel mobility-shift assay by Senear, D F, Brenowitz, M

“…We have investigated the question of whether the gel mobility-shift assay can provide data that are useful to the demonstration of cooperativity in the…”
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Comparison of operator-specific and nonspecific DNA binding of the .lambda. cI repressor: [KCl] and pH effects by Senear, Donald F, Batey, Robert

“…The effects of proton and KCl activity on the nonspecific lambda cI repressor-DNA interactions and on the site-specific repressor-O(R) interactions were…”
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The primary self-assembly reaction of bacteriophage .lambda. cI repressor dimers is to octamer by Senear, Donald F, Laue, Thomas M, Ross, J. B. Alexander, Waxman, Evan, Eaton, Steven, Rusinova, Elena

“…Cooperative binding of the bacteriophage lambda cI repressor dimer to specific sites of the phage operators OR and OL controls the developmental state of the…”
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5-Hydroxytryptophan as a new intrinsic probe for investigating protein-DNA interactions by analytical ultracentrifugation. Study of the effect of DNA on self-assembly of the bacteriophage .lambda. cI repressor by Laue, Thomas M, Senear, Donald F, Eaton, Steven, Ross, J. B. Alexander

“…Pairwise cooperativity between proteins bound to DNA is believed to be important in governing the transcriptional regulation of numerous genes. However, the…”
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Energetics of cooperative protein-DNA interactions: comparison between quantitative deoxyribonuclease footprint titration and filter binding by Senear, Donald F, Brenowitz, Michael, Shea, Madeline A, Ackers, Gary K

“…Using the binding of cI repressor protein to the lambda right and left operators as a model system, we have analyzed the two common experimental techniques for…”
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Proton-linked contributions to site-specific interactions of .lambda. cI repressor and OR by Senear, Donald F, Ackers, Gary K

“…The effects of proton activity on the site-specific interactions of cI repressors with operator sites OR were studied by using DNase I footprint titration…”
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Flanking DNA-sequences contribute to the specific binding of cI-repressor and OR1 by BRENOWITZ, M, SENEAR, D. F, ACKERS, G. K

“…The binding of cI-repressor to a series of mutant operators containing OR1 of the right operator of bacteriophage lambda was investigated. Sites OR2 and/or OR3…”
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Role of Protein−Protein Bridging Interactions on Cooperative Assembly of DNA-Bound CRP−CytR−CRP Complex and Regulation of the Escherichia Coli CytR Regulon by Chahla, Mayy, Wooll, John, Laue, Thomas M, Nguyen, Nghia, Senear, Donald F

“…The unlinked operons that comprise the Escherichia coli CytR regulon are controlled coordinately through interactions between two gene regulatory proteins, the…”
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Thermodynamics of E. coli cytidine repressor interactions with DNA: distinct modes of binding to different operators suggests a role in differential gene regulation by Tretyachenko-Ladokhina, Vira, Ross, J.B.Alexander, Senear, Donald F

“…Interactions between the Escherichia coli cytidine repressor protein (CytR) and its operator sites at the different promoters that comprise the CytR regulon,…”
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Activation of Gene Expression by a Ligand-induced Conformational Change of a Protein-DNA Complex by Rhee, Kyu Y., Senear, Donald F., Hatfield, G. Wesley

“…IlvY protein binds cooperatively to tandem operator sites in the divergent, overlapping, promoter-regulatory region of the ilvYC operon of Escherichia coli…”
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Role of multiple CytR binding sites on cooperativity, competition, and induction at the Escherichia coli udp promoter by Gavigan, S A, Nguyen, T, Nguyen, N, Senear, D F

“…The CytR repressor fulfills dual roles as both a repressor of transcription from promoters of the Escherichia coli CytR regulon and a co-activator in some…”
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Allosteric mechanism of induction of CytR-regulated gene expression. Cytr repressor-cytidine interaction by Barbier, C S, Short, S A, Senear, D F

“…Transcription from cistrons of the Escherichia coli CytR regulon is activated by E. coli cAMP receptor protein (CRP) and repressed by a multiprotein complex…”
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Quantitative DNase footprint titration: a method for studying protein-DNA interactions by Brenowitz, M, Senear, D F, Shea, M A, Ackers, G K

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Multiple ion-dependent and substrate-dependent Na+/K+-ATPase conformational states. Transient and steady-state kinetic studies by SENEAR, D. F, BETTS, G, BERNHARD, S. A

“…The hydrolysis of beta-(2-furyl)acryloyl phosphate (FAP), catalyzed by the Na+/K+-ATPase, is faster than the catalyzed hydrolysis of ATP. This is due to…”
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Linkage between Operator Binding and Dimer to Octamer Self-Assembly of Bacteriophage λ cI Repressor by Rusinova, Elena, Ross, J. B. Alexander, Laue, Thomas M, Sowers, Lawrence C, Senear, Donald F

“…Cooperative binding of the bacteriophage λ cI repressor dimer to specific sites of the phage operators OR and OL controls the developmental state of the phage…”
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Analysis of Protein and DNA-Mediated Contributions to Cooperative Assembly of Protein–DNA Complexes by Senear, Donald F., Ross, J.B.Alexander, Laue, Thomas M.

“…The cooperative assembly of protein–DNA complexes is a widespread phenomenon that is of particular significance to transcriptional regulation. Assembly of…”
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Thermodynamic investigation of monoclonal antibody alkylated nucleoside interaction as a model for epitope recognition on nucleic acids by Yuhasz, Stacieann C, Senear, Donald F, Adamkiewicz, Jurgen, Rajewsky, Manfred F, Ts'o, Paul O. P, Kan, Lousing

“…The objective of this investigation is examination of the dominant forces that govern complex formation between a series of monoclonal antibodies directed…”
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Thermodynamic studies of concanavalin a dimer-tetramer equilibria by Senear, D.F., Teller, D.C.

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