Age-related oxidative inactivation of Na +, K +-ATPase in rat brain crude synaptosomes

The study was undertaken to examine the status of Na +, K +-ATPase in aged rat brain and to verify if any alteration of this enzyme in aged brain could be related to an oxidative damage. The crude synaptosomes from rat brain were exposed in vitro to an oxidative stress in the form of a combination o...

Full description

Saved in:

Bibliographic Details
Published in:	Experimental gerontology Vol. 38; no. 6; pp. 705 - 710
Main Authors:	Chakraborty, Hemontika, Sen, Papparani, Sur, Arghya, Chatterjee, Uttara, Chakrabarti, Sasanka
Format:	Journal Article
Language:	English
Published:	England Elsevier Inc 01-06-2003
Subjects:	Aging - metabolism Animals Brain - enzymology Brain aging Fluorescent peroxidation products Lipid Peroxidation Na +, K +-ATPase Oxidative damage Oxidative Stress - physiology Protein carbonyls Rats Sodium-Potassium-Exchanging ATPase - metabolism Synaptosomes Synaptosomes - enzymology Brain aging Na +, K +-ATPase Synaptosomes Fluorescent peroxidation products Oxidative damage Protein carbonyls
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	The study was undertaken to examine the status of Na +, K +-ATPase in aged rat brain and to verify if any alteration of this enzyme in aged brain could be related to an oxidative damage. The crude synaptosomes from rat brain were exposed in vitro to an oxidative stress in the form of a combination of Fe 2+ (100 μM) and ascorbate (2 mM) for up to 2 h when increased lipid peroxidation (nearly four-fold), extensive protein carbonyl formation and a marked decrease of Na +, K +-ATPase activity (approximately 88%) were observed. All these changes were prevented by the presence of a chain-breaking anti-oxidant, butylated hydroxytoluene (0.2 mM), in the incubation mixture. When the same crude synaptosomal membranes from the young (4–6 months) and aged (18–22 months) rat brains were analysed, a significant reduction of Na +, K +-ATPase activity (nearly 48%) along with significantly elevated levels of lipid peroxidation products and protein carbonyls could be detected in the aged animals in comparison to young ones. The latter data in combination with the results of in vitro experiments imply that the age-related decline of rat brain Na +, K +-ATPase activity is presumably the consequence of an enhanced oxidative damage in aging brain
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0531-5565 1873-6815
DOI:	10.1016/S0531-5565(03)00066-4