Search Results - "Sellick, Christopher A"

Metabolite extraction from suspension-cultured mammalian cells for global metabolite profiling by Sellick, Christopher A, Hansen, Rasmus, Stephens, Gill M, Goodacre, Royston, Dickson, Alan J

“…Metabolite profiling of industrially important suspension-cultured mammalian cells is being increasingly used for rational improvement of bioprocesses. This…”
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At-line high throughput site-specific glycan profiling using targeted mass spectrometry by Chi, Bertie, Veyssier, Christel, Kasali, Toyin, Uddin, Faisal, Sellick, Christopher A.

“…•High throughput, site-specific glycan profiling using targeted mass spectrometry.•Rapid analysis of glycan profiles directly from culture media.•Methodology…”
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Eukaryotic transcription factors as direct nutrient sensors by Sellick, Christopher A., Reece, Richard J.

“…The recognition of changes in environmental conditions, and the ability to adapt to these changes, is essential for the viability of cells. There are numerous…”
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Molecular Structure of Saccharomyces cerevisiae Gal1p, a Bifunctional Galactokinase and Transcriptional Inducer by Thoden, James B., Sellick, Christopher A., Timson, David J., Reece, Richard J., Holden, Hazel M.

“…Gal1p of Saccharomyces cerevisiae is capable of performing two independent cellular functions. First, it is a key enzyme in the Leloir pathway for galactose…”
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The Effect of Ligand Binding on the Galactokinase Activity of Yeast Gal1p and Its Ability to Activate Transcription by Sellick, Christopher A., Jowitt, Thomas A., Reece, Richard J.

“…The galactokinase from Saccharomyces cerevisiae (ScGal1p) is a bifunctional protein. It is an enzyme responsible for the conversion of α-d-galactose into…”
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Contribution of Amino Acid Side Chains to Sugar Binding Specificity in a Galactokinase, Gal1p, and a Transcriptional Inducer, Gal3p by Sellick, Christopher A., Reece, Richard J.

“…The crystal structure of the yeast galactokinase, Gal1p, in the presence of its substrates has been solved recently. We systematically mutated each of the…”
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Pragmatic mAb lead molecule engineering from a developability perspective by Chi, Bertie, De Oliveira, Geoffrey, Gallagher, Tom, Mitchell, Laura, Knightley, Louise, Gonzalez, Cynthia C., Russell, Shannon, Germaschewski, Volker, Pearce, Claire, Sellick, Christopher A.

“…As the number of antibody drugs being approved and marketed increases, our knowledge of what makes potential drug candidates a successful product has increased…”
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Metabolite profiling of recombinant CHO cells: Designing tailored feeding regimes that enhance recombinant antibody production by Sellick, Christopher A., Croxford, Alexandra S., Maqsood, Arfa R., Stephens, Gill, Westerhoff, Hans V., Goodacre, Royston, Dickson, Alan J.

“…Chinese hamster ovary (CHO) cells are the primary platform for commercial expression of recombinant therapeutic proteins. Obtaining maximum production from the…”
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Effective Quenching Processes for Physiologically Valid Metabolite Profiling of Suspension Cultured Mammalian Cells by Sellick, Christopher A, Hansen, Rasmus, Maqsood, Arfa R, Dunn, Warwick B, Stephens, Gillian M, Goodacre, Royston, Dickson, Alan J

“…Global metabolite analysis approaches, coupled with sophisticated data analysis and modeling procedures (metabolomics), permit a dynamic read-out of how…”
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Metabolite profiling of CHO cells: Molecular reflections of bioprocessing effectiveness by Sellick, Christopher A., Croxford, Alexandra S., Maqsood, Arfa R., Stephens, Gill M., Westerhoff, Hans V., Goodacre, Royston, Dickson, Alan J.

“…Whilst development of medium and feeds has provided major advances in recombinant protein production in CHO cells, the fundamental understanding is limited. We…”
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The challenge of applying Raman spectroscopy to monitor recombinant antibody production by Ashton, Lorna, Xu, Yun, Brewster, Victoria L, Cowcher, David P, Sellick, Christopher A, Dickson, Alan J, Stephens, Gill M, Goodacre, Royston

“…UV resonance Raman (UVRR) spectroscopy combined with chemometric techniques was investigated as a physiochemical tool for monitoring secreted recombinant…”
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Rapid monitoring of recombinant antibody production by mammalian cell cultures using fourier transform infrared spectroscopy and chemometrics by Sellick, Christopher A, Hansen, Rasmus, Jarvis, Roger M, Maqsood, Arfa R, Stephens, Gill M, Dickson, Alan J, Goodacre, Royston

“…Fourier transform infrared (FT-IR) spectroscopy combined with multivariate statistical analyses was investigated as a physicochemical tool for monitoring…”
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Evaluation of extraction processes for intracellular metabolite profiling of mammalian cells: matching extraction approaches to cell type and metabolite targets by Sellick, Christopher A, Knight, David, Croxford, Alexandra S, Maqsood, Arfa R, Stephens, Gill M, Goodacre, Royston, Dickson, Alan J

“…In this study we report on the optimisation of the technologies for generation of a global metabolomics profile for intracellular metabolites in Chinese…”
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Metabolomics reveals the physiological response of Pseudomonas putida KT2440 (UWC1) after pharmaceutical exposure by Currie, Felicity, Broadhurst, David I, Dunn, Warwick B, Sellick, Christopher A, Goodacre, Royston

“…Human pharmaceuticals have been detected in wastewater treatment plants, rivers, and estuaries throughout Europe and the United States. It is widely…”
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Chapter 3 Galactose Metabolism in Yeast—Structure and Regulation of the Leloir Pathway Enzymes and the Genes Encoding Them by Sellick, Christopher A., Campbell, Robert N., Reece, Richard J.

“…The enzymes of the Leloir pathway catalyze the conversion of galactose to a more metabolically useful version, glucose‐6‐phosphate. This pathway is required as…”
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Galactose Metabolism in Yeast-Structure and Regulation of the Leloir Pathway Enzymes and the Genes Encoding them by Sellick, Christopher A, Campbell, Robert N, Reece, Richard J

“…The enzymes of the Leloir pathway catalyze the conversion of galactose to a more metabolically useful version, glucose-6-phosphate. This pathway is required as…”
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Rapid characterization of N‐linked glycans from secreted and gel‐purified monoclonal antibodies using MALDI‐ToF mass spectrometry by Hansen, Rasmus, Dickson, Alan J, Goodacre, Royston, Stephens, Gill M, Sellick, Christopher A

“…Recombinant monoclonal antibodies (MAbs) are increasingly being used for therapeutic use and correct glycosylation of these MAbs is essential for their correct…”
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Understanding a Transcriptional Paradigm at the Molecular Level: THE STRUCTURE OF YEAST Gal80p by Thoden, James B, Sellick, Christopher A, Reece, Richard J, Holden, Hazel M

“…In yeast, the GAL genes encode the enzymes required for normal galactose metabolism. Regulation of these genes in response to the organism being challenged…”
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Modulation of transcription factor function by an amino acid: activation of Put3p by proline by Sellick, Christopher A., Reece, Richard J.

“…Saccharomyces cerevisiae are able to convert proline to glutamate so that it may be used as a source of nitrogen. Here, we show that the activator of the…”
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Understanding a Transcriptional Paradigm at the Molecular Level by Thoden, James B., Sellick, Christopher A., Reece, Richard J., Holden, Hazel M.

“…In yeast, the GAL genes encode the enzymes required for normal galactose metabolism. Regulation of these genes in response to the organism being challenged…”
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