Assessment and Comparison of the Overall Analytical Potential of Capillary Electrophoresis and High-Performance Liquid Chromatography Using the RGB Model: How Much Can We Find Out?

Assessment and Comparison of the Overall Analytical Potential of Capillary Electrophoresis and High-Performance Liquid Chromatography Using the RGB Model: How Much Can We Find Out?

The choice between capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC) is not easy and depends on many factors. An attempt to facilitate this choice is this work, in which both techniques have been confronted on the basis of the RGB model, offering a transparent and pict...

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Bibliographic Details
Published in:Chromatographia Vol. 83; no. 9; pp. 1133 - 1144
Main Authors: Nowak, Paweł Mateusz, Sekuła, Edyta, Kościelniak, Paweł
Format: Journal Article
Language:English
Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-09-2020
Springer Nature B.V
Subjects:
Analytical Chemistry
Capillary electrophoresis
Chemistry
Chemistry and Materials Science
Chromatography
Data processing
Electrophoresis
High performance liquid chromatography
Laboratory Medicine
Original
Pharmacy
Proteomics
High-performance liquid chromatography
Capillary electrophoresis
Micellar electrokinetic chromatography
Capillary coating
RGB model
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Summary:The choice between capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC) is not easy and depends on many factors. An attempt to facilitate this choice is this work, in which both techniques have been confronted on the basis of the RGB model, offering a transparent and pictorial way to compare individual parameters as well as the overall analytical potential of each tool. To ensure the universal nature of the comparison, a simple in composition and chemically diverse model sample was used, accompanied by the data processing method reducing the potential impact of analyte selection. Moreover, permanent coating of the inner surface of the capillary and addition of a surfactant to the separation buffer were considered as the additional factors that may affect the assessment of the CE technique. The presented analysis can be valuable in any discussions about the intrinsic advantages and disadvantages of CE and HPLC, and divagations on how they affect the overall potential and usability of each. We also provide access to the Excel worksheets used for the assessment, which can be easily modified to reevaluate the methods with a different selection of variables, and analyze other possible scenarios.
ISSN:0009-5893
1612-1112
DOI:10.1007/s10337-020-03933-9