Search Results - "Seio, Kohji"

Quantification of synthetic errors during chemical synthesis of DNA and its suppression by non-canonical nucleosides by Masaki, Yoshiaki, Onishi, Yukiko, Seio, Kohji

“…Substitutions, insertions, and deletions derived from synthetic oligonucleotides are the hurdles for the synthesis of long DNA such as genomes. We quantified…”
Get full text

Selective and stable base pairing by alkynylated nucleosides featuring a spatially-separated recognition interface by Okamura, Hidenori, Trinh, Giang Hoang, Dong, Zhuoxin, Masaki, Yoshiaki, Seio, Kohji, Nagatsugi, Fumi

“…Abstract Unnatural base pairs (UBPs) which exhibit a selectivity against pairing with canonical nucleobases provide a powerful tool for the development of…”
Get full text

Synthesis of 2′-O-[3-(N-methylsulfamoyl)propan-1-yl]ribothymidine as a potentially applicable 2′-modified nucleoside for antisense oligonucleotides by Tomori, Takahito, Uekusa, Koya, Koyama, Aya, Kanagawa, Takayuki, Masaki, Yoshiaki, Seio, Kohji

“…[Display omitted] A synthetic scheme was developed to derive a modified ribothymidine bearing a 3-(N-methylsulfamoyl)propyl group on 2′-oxygen (TMSP). For…”
Get full text

A theoretical study on the elimination reaction of acrylonitrile from 2′-O-cyanoethylated nucleosides by Bu4NF by Ando, Kaori, Saneyoshi, Hisao, Seio, Kohji, Sekine, Mitsuo

“…The elimination reaction of acrylonitrile from 2′-O-cyanoethylated nucleosides by Bu4NF was studied computationally. The transition structures for the reaction…”
Get full text

Novel EGFP reporter cell and mouse models for sensitive imaging and quantification of exon skipping by Hara, Yuko, Mizobe, Yoshitaka, Inoue, Yukiko U., Hashimoto, Yasumasa, Motohashi, Norio, Masaki, Yoshiaki, Seio, Kohji, Takeda, Shin’ichi, Nagata, Tetsuya, Wood, Matthew J. A., Inoue, Takayoshi, Aoki, Yoshitsugu

“…Duchenne muscular dystrophy (DMD) is a fatal X-linked disorder caused by nonsense or frameshift mutations in the DMD gene. Among various treatments available…”
Get full text

Synthesis of photocaged 6-O-(2-nitrobenzyl)guanosine and 4-O-(2-nitrobenzyl) uridine triphosphates for photocontrol of the RNA transcription reaction by Ohno, Kentaro, Sugiyama, Daiki, Takeshita, Leo, Kanamori, Takashi, Masaki, Yoshiaki, Sekine, Mitsuo, Seio, Kohji

“…[Display omitted] 6-O-(2-Nitrobenzyl)guanosine and 4-O-(2-nitrobenzyl)uridine triphosphates (NBGTP, NBUTP) were synthesized, and their biochemical and…”
Get full text

Deoxynucleoside Triphosphate Containing Pyridazin-3-one Aglycon as a Thymidine Triphosphate Substitute for Primer Extension and Chain Elongation by Klenow Fragments by Tomori, Takahito, Nagaoka, Kento, Takeshita, Leo, Shiozawa, Takashi, Miyatake, Yuya, Masaki, Yoshiaki, Sekine, Mitsuo, Seio, Kohji

“…Deoxynucleoside 5′-triphosphate was synthesized with 3-oxo-2H-pyridazin-6-yl (PzO)a uracil analogue lacking a 2-keto groupas the nucleobase. Theoretical…”
Get full text

Synthesis and triplex-forming properties of oligonucleotides capable of recognizing corresponding DNA duplexes containing four base pairs by Ohkubo, Akihiro, Yamada, Kenji, Ito, Yu, Yoshimura, Kiichi, Miyauchi, Koichiro, Kanamori, Takashi, Masaki, Yoshiaki, Seio, Kohji, Yuasa, Hideya, Sekine, Mitsuo

“…A triplex-forming oligonucleotide (TFO) could be a useful molecular tool for gene therapy and specific gene modification. However, unmodified TFOs have two…”
Get full text

Synthesis of and triplex formation in oligonucleotides containing 2′-deoxy-6-thioxanthosine by Inde, Takeshi, Nishizawa, Shuhei, Hattori, Yuusaku, Kanamori, Takashi, Yuasa, Hideya, Seio, Kohji, Sekine, Mitsuo, Ohkubo, Akihiro

“…[Display omitted] This study aimed to synthesize triplex-forming oligonucleotides (TFOs) containing 2′-deoxy-6-thioxanthosine (s6X) and…”
Get full text

Crystal structure of a DNA duplex cross-linked by 6-thioguanine-6-thioguanine disulfides: reversible formation and cleavage catalyzed by Cu() ions and glutathione by Ono, Akira, Atsugi, Takahiro, Goto, Misato, Saneyoshi, Hisao, Tomori, Takahito, Seio, Kohji, Dairaku, Takenori, Kondo, Jiro

“…Herein, we determined the crystal structure of a DNA duplex containing consecutive 6-thioguanine-6-thioguanine disulfides. The disulfide bonds were reversibly…”
Get full text

Hic-5 antisense oligonucleotide inhibits advanced hepatic fibrosis and steatosis in vivo by Noguchi, Masahito, Miyauchi, Aya, Masaki, Yoshiaki, Sakaki, Masashi, Lei, Xiao-Feng, Kobayashi-Tanabe, Momoko, Miyazaki, Akira, Aoki, Takeshi, Yoshida, Hitoshi, Seio, Kohji, Kim-Kaneyama, Joo-ri

“…Chronic liver diseases, including metabolic dysfunction-associated steatohepatitis (MASH), pose a significant global health burden. Progressive liver fibrosis…”
Get full text

Synthesis of 2′-O-(N-methylcarbamoylethyl) 5-methyl-2-thiouridine and its application to splice-switching oligonucleotides by Masaki, Yoshiaki, Yamamoto, Keishi, Inde, Takeshi, Yoshida, Keita, Maruyama, Atsuya, Nagata, Tetsuya, Tanihata, Jun, Takeda, Shin'ichi, Sekine, Mitsuo, Seio, Kohji

“…[Display omitted] The effect of 2′-O-(N-methylcarbamoyl)ethyl (MCE) modification on splice-switching oligonucleotides (SSO) was systematically evaluated. The…”
Get full text

A General Method for the Synthesis of 2‘-O-Cyanoethylated Oligoribonucleotides Having Promising Hybridization Affinity for DNA and RNA and Enhanced Nuclease Resistance by Saneyoshi, Hisao, Seio, Kohji, Sekine, Mitsuo

“…An effective method for the synthesis of 2‘-O-cyanoethylated oligoribonucleotides as a new class of 2‘-O-modified RNAs was developed. The reaction of…”
Get full text

Controlling the Fluorescence of Benzofuran-Modified Uracil Residues in Oligonucleotides by Triple-Helix Formation by Kanamori, Takashi, Ohzeki, Hiroki, Masaki, Yoshiaki, Ohkubo, Akihiro, Takahashi, Mari, Tsuda, Kengo, Ito, Takuhiro, Shirouzu, Mikako, Kuwasako, Kanako, Muto, Yutaka, Sekine, Mitsuo, Seio, Kohji

“…We developed fluorescent turn‐on probes containing a fluorescent nucleoside, 5‐(benzofuran‐2‐yl)deoxyuridine (dUBF) or 5‐(3‐methylbenzofuran‐2‐yl)deoxyuridine…”
Get full text

Enzymatic synthesis and reverse transcription of RNAs incorporating 2'-O-carbamoyl uridine triphosphate by Masaki, Yoshiaki, Ito, Hyugo, Oda, Yuki, Yamazaki, Kazufumi, Tago, Nobuhiro, Ohno, Kentaro, Ishii, Nozomi, Tsunoda, Hirosuke, Kanamori, Takashi, Ohkubo, Akihiro, Sekine, Mitsuo, Seio, Kohji

“…Enzymatic synthesis and the reverse transcription of RNAs containing 2'-O-carbamoyl uridine were evaluated. A mild acidic deprotection procedure allowed the…”
Get more information

Photo-controlled binding of MutS to photo-caged DNA duplexes incorporating 4-O-(2-nitrobenzyl) or 4-O-[2-(2-nitrophenyl)propyl]thymidine by Seio, Kohji, Ohno, Yurie, Ohno, Kentaro, Takeshita, Leo, Kanamori, Takashi, Masaki, Yoshiaki, Sekine, Mitsuo

“…[Display omitted] Mismatch binding protein MutS binding to bulge structure in DNA duplexes was controlled by UV irradiation. 4-O-(2-Nitrobenzyl)thymidine or…”
Get full text

Effective Strategy for Conformer-Selective Detection of Short-Lived Excited State Species: Application to the IR Spectroscopy of the N1H Keto Tautomer of Guanine by Asami, Hiroya, Tokugawa, Munefumi, Masaki, Yoshiaki, Ishiuchi, Shun-ichi, Gloaguen, Eric, Seio, Kohji, Saigusa, Hiroyuki, Fujii, Masaaki, Sekine, Mitsuo, Mons, Michel

“…The ultrafast deactivation processes in the excited state of biomolecules, such as the most stable tautomers of guanine, forbid any state-of-the-art gas phase…”
Get full text

Linear Relationship between Deformability and Thermal Stability of 2′-O-Modified RNA Hetero Duplexes by Masaki, Yoshiaki, Miyasaka, Ryuta, Ohkubo, Akihiro, Seio, Kohji, Sekine, Mitsuo

“…We describe the relationship between the experimentally determined melting temperatures of 2′-O-modified-RNA/RNA duplexes and their deformability estimated…”
Get full text

Synthesis of Responsive Fluorescent Nucleobases 7-(Benzofuran-2-yl)-7-deazahypoxanthine and 7-(Benzofuran-2-yl)-7-deazaguanine Using Cross-coupling Reaction by Tokugawa, Munefumi, Kaneko, Kazuhei, Saito, Masanori, Kanamori, Takashi, Masaki, Yoshiaki, Ohkubo, Akihiro, Sekine, Mitsuo, Seio, Kohji

“…In order to develop fluorescent guanine analogs having substitutions at the 7-positions, 7-(benzofuran-2-yl)-7-deazahypoxanthine (1a) and…”
Get full text

Development of an efficient method for phosphorodiamidate bond formation by using inorganic salts by Harakawa, Taro, Tsunoda, Hirosuke, Ohkubo, Akihiro, Seio, Kohji, Sekine, Mitsuo

“…Phosphorodiamidate morpholino oligonucleotides (PMOs) have been extensively applied in antisense strategies for gene regulation because of their high stability…”
Get full text