Search Results - "Seidel, Claus A. M"

Phase-separating RNA-binding proteins form heterogeneous distributions of clusters in subsaturated solutions by Kar, Mrityunjoy, Dar, Furqan, Welsh, Timothy J, Vogel, Laura T, Kühnemuth, Ralf, Majumdar, Anupa, Krainer, Georg, Franzmann, Titus M, Alberti, Simon, Seidel, Claus A M, Knowles, Tuomas P J, Hyman, Anthony A, Pappu, Rohit V

“…Macromolecular phase separation is thought to be one of the processes that drives the formation of membraneless biomolecular condensates in cells. The dynamics…”
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Resolving dynamics and function of transient states in single enzyme molecules by Sanabria, Hugo, Rodnin, Dmitro, Hemmen, Katherina, Peulen, Thomas-Otavio, Felekyan, Suren, Fleissner, Mark R., Dimura, Mykola, Koberling, Felix, Kühnemuth, Ralf, Hubbell, Wayne, Gohlke, Holger, Seidel, Claus A. M.

“…We use a hybrid fluorescence spectroscopic toolkit to monitor T4 Lysozyme (T4L) in action by unraveling the kinetic and dynamic interplay of the conformational…”
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Dynamic anticipation by Cdk2/Cyclin A-bound p27 mediates signal integration in cell cycle regulation by Tsytlonok, Maksym, Sanabria, Hugo, Wang, Yuefeng, Felekyan, Suren, Hemmen, Katherina, Phillips, Aaron H., Yun, Mi-Kyung, Waddell, M. Brett, Park, Cheon-Gil, Vaithiyalingam, Sivaraja, Iconaru, Luigi, White, Stephen W., Tompa, Peter, Seidel, Claus A. M., Kriwacki, Richard

“…p27 Kip1 is an intrinsically disordered protein (IDP) that inhibits cyclin-dependent kinase (Cdk)/cyclin complexes (e.g., Cdk2/cyclin A), causing cell cycle…”
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Automated and optimally FRET-assisted structural modeling by Dimura, Mykola, Peulen, Thomas-Otavio, Sanabria, Hugo, Rodnin, Dmitro, Hemmen, Katherina, Hanke, Christian A., Seidel, Claus A. M., Gohlke, Holger

“…FRET experiments can provide state-specific structural information of complex dynamic biomolecular assemblies. However, to overcome the sparsity of FRET…”
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An optofluidic antenna for enhancing the sensitivity of single-emitter measurements by Morales-Inostroza, Luis, Folz, Julian, Kühnemuth, Ralf, Felekyan, Suren, Wieser, Franz-Ferdinand, Seidel, Claus A. M., Götzinger, Stephan, Sandoghdar, Vahid

“…Many single-molecule investigations are performed in fluidic environments, for example, to avoid unwanted consequences of contact with surfaces. Diffusion of…”
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A blind benchmark of analysis tools to infer kinetic rate constants from single-molecule FRET trajectories by Götz, Markus, Barth, Anders, Bohr, Søren S.-R., Börner, Richard, Chen, Jixin, Cordes, Thorben, Erie, Dorothy A., Gebhardt, Christian, Hadzic, Mélodie C. A. S., Hamilton, George L., Hatzakis, Nikos S., Hugel, Thorsten, Kisley, Lydia, Lamb, Don C., de Lannoy, Carlos, Mahn, Chelsea, Dunukara, Dushani, de Ridder, Dick, Sanabria, Hugo, Schimpf, Julia, Seidel, Claus A. M., Sigel, Roland K. O., Sletfjerding, Magnus Berg, Thomsen, Johannes, Vollmar, Leonie, Wanninger, Simon, Weninger, Keith R., Xu, Pengning, Schmid, Sonja

“…Single-molecule FRET (smFRET) is a versatile technique to study the dynamics and function of biomolecules since it makes nanoscale movements detectable as…”
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Structural heterogeneity and quantitative FRET efficiency distributions of polyprolines through a hybrid atomistic simulation and Monte Carlo approach by Hoefling, Martin, Lima, Nicola, Haenni, Dominik, Seidel, Claus A M, Schuler, Benjamin, Grubmüller, Helmut

“…Förster Resonance Energy Transfer (FRET) experiments probe molecular distances via distance dependent energy transfer from an excited donor dye to an acceptor…”
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Solutes unmask differences in clustering versus phase separation of FET proteins by Kar, Mrityunjoy, Vogel, Laura T., Chauhan, Gaurav, Felekyan, Suren, Ausserwöger, Hannes, Welsh, Timothy J., Dar, Furqan, Kamath, Anjana R., Knowles, Tuomas P. J., Hyman, Anthony A., Seidel, Claus A. M., Pappu, Rohit V.

“…Phase separation and percolation contribute to phase transitions of multivalent macromolecules. Contributions of percolation are evident through the…”
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Filtered FCS: Species Auto- and Cross-Correlation Functions Highlight Binding and Dynamics in Biomolecules by Felekyan, Suren, Kalinin, Stanislav, Sanabria, Hugo, Valeri, Alessandro, Seidel, Claus A. M.

“…An analysis method of lifetime, polarization and spectrally filtered fluorescence correlation spectroscopy, referred to as filtered FCS (fFCS), is introduced…”
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Enhanced Solid-State Fluorescence of Flavin Derivatives by Incorporation in the Metal-Organic Frameworks MIL-53(Al) and MOF-5 by Püschel, Dietrich, Hédé, Simon, Maisuls, Iván, Höfert, Simon-Patrick, Woschko, Dennis, Kühnemuth, Ralf, Felekyan, Suren, Seidel, Claus A M, Czekelius, Constantin, Weingart, Oliver, Strassert, Cristian A, Janiak, Christoph

“…The flavin derivatives 10-methyl-isoalloxazine (MIA) and 6-fluoro-10-methyl-isoalloxazine (6F-MIA) were incorporated in two alternative metal-organic…”
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Reply to: On the statistical foundation of a recent single molecule FRET benchmark by Götz, Markus, Barth, Anders, Bohr, Søren S. -R., Börner, Richard, Chen, Jixin, Cordes, Thorben, Erie, Dorothy A., Gebhardt, Christian, Hadzic, Mélodie C. A. S., Hamilton, George L., Hatzakis, Nikos S., Hugel, Thorsten, Kisley, Lydia, Lamb, Don C., de Lannoy, Carlos, Mahn, Chelsea, Dunukara, Dushani, de Ridder, Dick, Sanabria, Hugo, Schimpf, Julia, Seidel, Claus A. M., Sigel, Roland K. O., Sletfjerding, Magnus B., Thomsen, Johannes, Vollmar, Leonie, Wanninger, Simon, Weninger, Keith R., Xu, Pengning, Schmid, Sonja

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Separating Structural Heterogeneities from Stochastic Variations in Fluorescence Resonance Energy Transfer Distributions via Photon Distribution Analysis by Antonik, Matthew, Felekyan, Suren, Gaiduk, Alexander, Seidel, Claus A. M

“…We establish a probability distribution analysis (PDA) method for the analysis of fluorescence resonance energy transfer (FRET) signals to determine with high…”
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On the Origin of Broadening of Single-Molecule FRET Efficiency Distributions beyond Shot Noise Limits by Kalinin, Stanislav, Sisamakis, Evangelos, Magennis, Steven W, Felekyan, Suren, Seidel, Claus A. M

“…Single-molecule FRET experiments on freely diffusing rigid molecules frequently show FRET efficiency (E) distributions broader than those defined by photon…”
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Supertertiary structure of the synaptic MAGuK scaffold proteins is conserved by McCann, James J, Zheng, Liqiang, Rohrbeck, Daniel, Felekyan, Suren, Kühnemuth, Ralf, Sutton, R. Bryan, Seidel, Claus A. M, Bowen, Mark E

“…Scaffold proteins form a framework to organize signal transduction by binding multiple partners within a signaling pathway. This shapes the output of signal…”
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Structural and dynamic insights revealing how lipase binding domain MD1 of Pseudomonas aeruginosa foldase affects lipase activation by Viegas, Aldino, Dollinger, Peter, Verma, Neha, Kubiak, Jakub, Viennet, Thibault, Seidel, Claus A. M., Gohlke, Holger, Etzkorn, Manuel, Kovacic, Filip, Jaeger, Karl-Erich

“…Folding and cellular localization of many proteins of Gram-negative bacteria rely on a network of chaperones and secretion systems. Among them is the…”
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Strategies to Improve Photostabilities in Ultrasensitive Fluorescence Spectroscopy by Widengren, Jerker, Chmyrov, Andriy, Eggeling, Christian, Löfdahl, Per-Åke, Seidel, Claus A. M

“…Given the particular importance of dye photostability for single-molecule and fluorescence fluctuation spectroscopy investigations, refined strategies were…”
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Nucleosome Disassembly Intermediates Characterized by Single-Molecule FRET by Gansen, Alexander, Valeri, Alessandro, Hauger, Florian, Felekyan, Suren, Kalinin, Stanislav, Tóth, Katalin, Langowski, Jörg, Seidel, Claus A. M., Workman, Jerry L.

“…The nucleosome has a central role in the compaction of genomic DNA and the control of DNA accessibility for transcription and replication. To help…”
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Molecular Photobleaching Kinetics of Rhodamine 6G by One- and Two-Photon Induced Confocal Fluorescence Microscopy by Eggeling, Christian, Volkmer, Andreas, Seidel, Claus A. M.

“…Under high‐excitation irradiance conditions in one‐ and two‐photon induced fluorescence microscopy, the photostability of fluorescent dyes is of crucial…”
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Single-molecule FRET measures bends and kinks in DNA by Woźniak, Anna K, Schröder, Gunnar F, Grubmüller, Helmut, Seidel, Claus A.M, Oesterhelt, Filipp

“…We present advances in the use of single-molecule FRET measurements with flexibly linked dyes to derive full 3D structures of DNA constructs based on absolute…”
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Analysis of Photobleaching in Single-Molecule Multicolor Excitation and Förster Resonance Energy Transfer Measurements by Eggeling, Christian, Widengren, Jerker, Brand, Leif, Schaffer, Jörg, Felekyan, Suren, Seidel, Claus A. M

“…Dye photobleaching is a major constraint of fluorescence readout within a range of applications. In this study, we investigated the influence of photobleaching…”
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