Chemical Composition and Biotechnological Properties of a Polysaccharide from the Peels and Antioxidative Content from the Pulp of Passiflora liguralis Fruits

A new polysaccharide with a high molecular weight (greater than 1 × 106 Da) was extracted and characterized from the peels of Passiflora liguralis (granadilla) fruits. Chemical composition of the biopolymer, performed by using a high pressure anion exchange-pulsed amperometric detector (HPAE-PAD), s...

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Bibliographic Details
Published in:	Journal of agricultural and food chemistry Vol. 55; no. 18; pp. 7427 - 7433
Main Authors:	Tommonaro, G, Segura Rodríguez, C. S, Santillana, M, Immirzi, B, De Prisco, R, Nicolaus, B, Poli, A
Format:	Journal Article
Language:	English
Published:	Washington, DC American Chemical Society 05-09-2007
Subjects:	antioxidant activity antioxidants Antioxidants - analysis Artemia salina avarol bioassays Biological and medical sciences Biotechnology carbohydrate composition Chemical Phenomena Chemistry, Physical cytotoxicity Dysidea avara films (materials) Food industries Food Packaging Fruit - chemistry Fruit and vegetable industries fruit pulp Fundamental and applied biological sciences. Psychology Glucans - analysis Glucans - pharmacology heat stability molecular weight Passiflora - chemistry Passiflora ligularis peels Polysaccharides - analysis Polysaccharides - chemistry Polysaccharides - pharmacology Porifera protective effect Sesquiterpenes - antagonists & inhibitors sesquiterpenoids Xylans - analysis Xylans - pharmacology xyloglucans Passion fruit biodegradable film Fruit Passifloraceae Film Passiflora edulis Antioxidant antioxidant activity Assay Dicotyledones Angiospermae biological assay Peel Spermatophyta Chemical composition Fruit pulp Chemical properties Polysaccharide Passiflora liguralis
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Summary:	A new polysaccharide with a high molecular weight (greater than 1 × 106 Da) was extracted and characterized from the peels of Passiflora liguralis (granadilla) fruits. Chemical composition of the biopolymer, performed by using a high pressure anion exchange-pulsed amperometric detector (HPAE-PAD), showed the presence of six different sugar residues: xylose, glucose, galactose, galactosamine, an unknown component, and fucose in the relative ratio of 1:0.5:0.2:0.06:0.05:trace. The optical rotation of this xyloglucan was [α]D 25 °C = −186.42 (concentration of 1.4 mg/mL of H2O), and the viscosity was dependent on the concentration and pH, showing a maximum value of 1.4 η at a concentration of 3% in distilled water and a maximum value of 7.0 η in citrate buffer solution. Thermogravimetric analysis indicated that this biopolymer was very stable at high temperatures, showing a degradation temperature at 280 °C. The characterization of the polysaccharide was also investigated by spectroscopic methods (1H NMR and IR) pointing out the complexity of this biopolymer and the presence of sugar residues in α-manno, α-gluco-galacto, and β-gluco-galacto configurations. The formation of a biodegradable film using this novel xyloglucan was reported, and the anticytotoxic activity of the polysaccharide was studied in a brine shrimp bioassay. Considerable antioxidant activity (Trolox equivalent antioxidant capacity (TEAC) value of 0.32 μM/mg fresh product) was noted in the lipophilic extracts of Passiflora liguralis fruits, indicating, in this fruit, an alternative source of bioactive compounds. Keywords: Passiflora liguralis; passion fruit; polysaccharide; biological assay; antioxidant activity; biodegradable film.
Bibliography:	http://dx.doi.org/10.1021/jf0704615 istex:4C9294649265A4F856033144078831F330DF8CF3 ark:/67375/TPS-4WZF10CZ-G ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0021-8561 1520-5118
DOI:	10.1021/jf0704615