IN VITRO IMMUNE-MODULATORY POTENTIAL OF CRUDE EXTRACT OF LEAF OF ALBIZIA GUMMIFERA AGAINST STIMULATED PERIPHERAL BLOOD MONONUCLEAR AND RAW CELLS
Background: Albizia species including A. GUMMIFERA have been used in folk medicine for the treatment of various conditions by many cultural groups. Apart from its anti-parasitic and antimicrobial activity associated with its use, Albizia gummifera have not been investigated for either its anti-infla...
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| Published in: | African journal of traditional, complementary, and alternative medicines Vol. 12; no. 3; p. 85 |
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| Main Authors: | , , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
African Ethnomedicines Network
01-01-2015
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | Background: Albizia species including A. GUMMIFERA have been used
in folk medicine for the treatment of various conditions by many
cultural groups. Apart from its anti-parasitic and antimicrobial
activity associated with its use, Albizia gummifera have not been
investigated for either its anti-inflammatory properties or
anti-proliferative effect in vitro. Materials and Methods: Extracted
acetone crude extract of plant material was tested for the presence of
various secondary metabolites using chemical tests while the
anti-proliferative effect against peripheral blood mononuclear were
tested using the WST-1 assay. Nitric oxide production by RAW cell line
exposed to extract was also tested using Griess assay as well as the
production of interleukin- 2 in medium of cultured PMBC using an
enzyme-linked-immunosorbent assay (ELISA) kit. Results: Phytochemical
testing of acetone leaf extracted was shown to be positive for
flavonoids, saponins and tannins. The extract was shown to
dose-dependently inhibited proliferation of mononuclear cells while
promoting that of RAW cells (p<0.05). The production of IL-2 by
mononuclear cells and nitric oxide release by RAW cells were inhibited
and stimulated respectively (p<0.05) in both cell types. The extract
was also shown to elicit significant anti- and pro-inflammatory
potential at concentration above 20 and 40 μg/ml, in both cell
types respectively. Conclusion: Further laboratory research is required
to elucidate the anti- and pro-inflammatory biochemical pathways as
shown by the in vitro immune-modulatory modalities of this plant
species. It is also important to further identify bioactive entities of
Albizia gummifera responsible for the observed activities. |
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| ISSN: | 0189-6016 0189-6016 |
| DOI: | 10.4314/ajtcam.v12i3.10 |