Search Results - "Sedat, John"

Helical coiled nucleosome chromosome architectures during cell cycle progression by McDonald, Angus, Murre, Cornelis, Sedat, John W

“…Recent studies showed an interphase chromosome architecture-a specific coiled nucleosome structure-derived from cryopreserved EM tomograms, and dispersed…”
Get full text

Three-dimensional deconvolution processing for STEM cryotomography by Waugh, Barnali, Wolf, Sharon G., Fass, Deborah, Branlund, Eric, Kam, Zvi, Sedat, John W., Elbaum, Michael

“…The complex environment of biological cells and tissues has motivated development of three-dimensional (3D) imaging in both light and electron microscopies. To…”
Get full text

Three-Dimensional Resolution Doubling in Wide-Field Fluorescence Microscopy by Structured Illumination by Gustafsson, Mats G.L., Shao, Lin, Carlton, Peter M., Wang, C. J. Rachel, Golubovskaya, Inna N., Cande, W. Zacheus, Agard, David A., Sedat, John W.

“…Structured illumination microscopy is a method that can increase the spatial resolution of wide-field fluorescence microscopy beyond its classical limit by…”
Get full text

Condensed mitotic chromosome structure at nanometer resolution using PALM and EGFP- histones by Matsuda, Atsushi, Shao, Lin, Boulanger, Jerome, Kervrann, Charles, Carlton, Peter M, Kner, Peter, Agard, David, Sedat, John W

“…Photoactivated localization microscopy (PALM) and related fluorescent biological imaging methods are capable of providing very high spatial resolutions (up to…”
Get full text

Subdiffraction Multicolor Imaging of the Nuclear Periphery with 3D Structured Illumination Microscopy by Schermelleh, Lothar, Carlton, Peter M, Haase, Sebastian, Shao, Lin, Winoto, Lukman, Kner, Peter, Burke, Brian, Cardoso, M. Cristina, Agard, David A, Gustafsson, Mats G.L, Leonhardt, Heinrich, Sedat, John W

“…Fluorescence light microscopy allows multicolor visualization of cellular components with high specificity, but its utility has until recently been constrained…”
Get full text

High-resolution restoration of 3D structures from widefield images with extreme low signal-to-noise-ratio by Arigovindan, Muthuvel, Fung, Jennifer C., Elnatan, Daniel, Mennella, Vito, Chan, Yee-Hung Mark, Pollard, Michael, Branlund, Eric, Sedat, John W., Agard, David A.

“…Four-dimensional fluorescence microscopy—which records 3D image information as a function of time—provides an unbiased way of tracking dynamic behavior of…”
Get full text

Fast live simultaneous multiwavelength four-dimensional optical microscopy by Carlton, Peter M., Boulanger, Jérôme, Kervrann, Charles, Sibarita, Jean-Baptiste, Salamero, Jean, Gordon-Messer, Susannah, Bressan, Debra, Haber, James E., Haase, Sebastian, Shao, Lin, Winoto, Lukman, Matsuda, Atsushi, Kner, Peter, Uzawa, Satoru, Gustafsson, Mats, Kam, Zvi, Agard, David A., Sedat, John W.

“…Live fluorescence microscopy has the unique capability to probe dynamic processes, linking molecular components and their localization with function. A key…”
Get full text

CryoSIM: super-resolution 3D structured illumination cryogenic fluorescence microscopy for correlated ultrastructural imaging by Phillips, Michael A, Harkiolaki, Maria, Susano Pinto, David Miguel, Parton, Richard M, Palanca, Ana, Garcia-Moreno, Manuel, Kounatidis, Ilias, Sedat, John W, Stuart, David I, Castello, Alfredo, Booth, Martin J, Davis, Ilan, Dobbie, Ian M

“…Rapid cryopreservation of biological specimens is the gold standard for visualizing cellular structures in their true structural context. However, current…”
Get full text

A Presynaptic Giant Ankyrin Stabilizes the NMJ through Regulation of Presynaptic Microtubules and Transsynaptic Cell Adhesion by Pielage, Jan, Cheng, Ling, Fetter, Richard D., Carlton, Pete M., Sedat, John W., Davis, Graeme W.

“…In a forward genetic screen for mutations that destabilize the neuromuscular junction, we identified a novel long isoform of Drosophila ankyrin2 ( ank2-L). We…”
Get full text

N-terminal processing of proteins exported by malaria parasites by Chang, Henry H., Falick, Arnold M., Carlton, Peter M., Sedat, John W., DeRisi, Joseph L., Marletta, Michael A.

“…Malaria parasites utilize a short N-terminal amino acid motif termed the Plasmodium export element (PEXEL) to export an array of proteins to the host…”
Get full text

Effect of depth dependent spherical aberrations in 3D structured illumination microscopy by Arigovindan, Muthuvel, Sedat, John W, Agard, David A

“…We model the effect of depth dependent spherical aberration caused by a refractive index mismatch between the mounting and immersion mediums in a 3D structured…”
Get full text

UCSF tomography: An integrated software suite for real-time electron microscopic tomographic data collection, alignment, and reconstruction by Zheng, Shawn Q., Keszthelyi, Bettina, Branlund, Eric, Lyle, John M., Braunfeld, Michael B., Sedat, John W., Agard, David A.

“…A real-time alignment and reconstruction scheme for electron microscopic tomography (EMT) has been developed and integrated within our UCSF tomography data…”
Get full text

A Parallel Product-Convolution approach for representing the depth varying Point Spread Functions in 3D widefield microscopy based on principal component analysis by Arigovindan, Muthuvel, Shaevitz, Joshua, McGowan, John, Sedat, John W, Agard, David A

“…We address the problem of computational representation of image formation in 3D widefield fluorescence microscopy with depth varying spherical aberrations. We…”
Get full text

Polarization of Chemoattractant Receptor Signaling during Neutrophil Chemotaxis by Servant, Guy, Weiner, Orion D., Herzmark, Paul, Balla, Tamás, Sedat, John W., Bourne, Henry R.

“…Morphologic polarity is necessary for chemotaxis of mammalian cells. As a probe of intracellular signals responsible for this asymmetry, the pleckstrin…”
Get full text

Evidence of activity-specific, radial organization of mitotic chromosomes in Drosophila by Strukov, Yuri G, Sural, Tûba H, Kuroda, Mitzi I, Sedat, John W

“…The organization and the mechanisms of condensation of mitotic chromosomes remain unsolved despite many decades of efforts. The lack of resolution, tight…”
Get full text

The Mcp element mediates stable long-range chromosome-chromosome interactions in Drosophila by Vazquez, Julio, Müller, Martin, Pirrotta, Vincenzo, Sedat, John W

“…Chromosome organization inside the nucleus is not random but rather is determined by a variety of factors, including interactions between chromosomes and…”
Get full text

Rapid telomere motions in live human cells analyzed by highly time-resolved microscopy by Wang, Xueying, Kam, Zvi, Carlton, Peter M, Xu, Lifeng, Sedat, John W, Blackburn, Elizabeth H

“…Telomeres cap chromosome ends and protect the genome. We studied individual telomeres in live human cancer cells. In capturing telomere motions using…”
Get full text

Modelling the application of adaptive optics to wide-field microscope live imaging by KAM, ZVI, KNER, PETER, AGARD, DAVID, SEDAT, JOHN W

“…Wide-field fluorescence microscopy is an essential tool in modern cell biology. Unfortunately the image quality of fluorescence microscopes is often…”
Get full text

AIDA: an adaptive image deconvolution algorithm with application to multi-frame and three-dimensional data by Hom, Erik F Y, Marchis, Franck, Lee, Timothy K, Haase, Sebastian, Agard, David A, Sedat, John W

“…We describe an adaptive image deconvolution algorithm (AIDA) for myopic deconvolution of multi-frame and three-dimensional data acquired through astronomical…”
Get more information

The Dynamics of Homologous Chromosome Pairing during Male Drosophila Meiosis by Vazquez, Julio, Belmont, Andrew S, Sedat, John W

“…Background: Meiotic pairing is essential for the proper orientation of chromosomes at the metaphase plate and their subsequent disjunction during anaphase I…”
Get full text