A scalable hyperthermic intravesical chemotherapy (HIVEC) setup for rat models of bladder cancer

A scalable hyperthermic intravesical chemotherapy (HIVEC) setup for rat models of bladder cancer

Hyperthermic intravesical chemotherapy (HIVEC)—whereby the bladder is heated to ± 43 °C during a chemotherapy instillation—can improve outcomes of non-muscle invasive bladder cancer (NMIBC) treatments. Experiments in animal models are required to explore new hyperthermia based treatments. Existing H...

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Bibliographic Details
Published in:Scientific reports Vol. 12; no. 1; p. 7017
Main Authors: Van Hattum, J. W., Scutigliani, E. M., Helderman, R. F. C. P. A., Zweije, R., Rodermond, H. M., Oei, A. L., Crezee, J., Oddens, J. R., De Reijke, T. M., Krawczyk, P. M.
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 29-04-2022
Nature Publishing Group
Nature Portfolio
Subjects:
631/67/589/1336
692/308/2778
Administration, Intravesical
Animal models
Animals
Bladder cancer
Catheters
Chemotherapy
Female
Hot Temperature
Humanities and Social Sciences
Humans
Hyperthermia
Hyperthermia, Induced
Invasiveness
Male
Medical instruments
multidisciplinary
Rats
Science
Science (multidisciplinary)
Urinary Bladder
Urinary Bladder Neoplasms - drug therapy
Water inflow
Water outflow
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Summary:Hyperthermic intravesical chemotherapy (HIVEC)—whereby the bladder is heated to ± 43 °C during a chemotherapy instillation—can improve outcomes of non-muscle invasive bladder cancer (NMIBC) treatments. Experiments in animal models are required to explore new hyperthermia based treatments. Existing HIVEC devices are not suitable for rodents or large-scale animal trials. We present a HIVEC setup compatible with orthotopic rat models . An externally heated chemotherapeutic solution is circulated in the bladder through a double-lumen catheter with flow rates controlled using a peristaltic pump. Temperature sensors in the inflow channel, bladder and outflow channel allow temperature monitoring and adjustments in real-time. At a constant flow rate of 2.5 mL/min the system rapidly reaches the desired bladder temperature of 42–43 °C with minimal variability throughout a one-hour treatment in a rat bladder phantom, as well as in euthanised and live rats. Mean intraluminal bladder temperatures were 42.92 °C (SD = 0.15 °C), 42.45 °C (SD = 0.37 °C) and 42.52 °C (SD = 0.09 °C) in the bladder phantom, euthanised, and live rats respectively. Thermal camera measurements showed homogenous heat distributions over the bladder wall. The setup provides well-controlled thermal dose and the upscaling needed for performing large scale HIVEC experiments in rats.
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-022-11016-y