Search Results - "Scriven, David R. L."

structure and functioning of the couplon in the mammalian cardiomyocyte by Asghari, Parisa, Scriven, David R. L, Hoskins, Jeremy, Fameli, Nicola, van Breemen, Cornelis, Moore, Edwin D. W

“…The couplons of the cardiomyocyte form nanospaces within the cell that place the L-type calcium channel (Cav1.2), situated on the plasmalemma, in opposition to…”
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Cardiomyocyte ryanodine receptor clusters expand and coalesce after application of isoproterenol by Scriven, David R L, Johnsen, Anne Berit, Asghari, Parisa, Chou, Keng C, Moore, Edwin D W

“…Earlier work has shown that ventricular ryanodine receptors (RyR2) within a cluster rearrange on phosphorylation as well as with a number of other stimuli…”
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Phosphorylation of RyR2 simultaneously expands the dyad and rearranges the tetramers by Asghari, Parisa, Scriven, David R L, Shahrasebi, Saba, Valdivia, Hector H, Alsina, Katherina M, Valdivia, Carmen R, Navarro-Garcia, J Alberto, Wehrens, Xander H T, Moore, Edwin D W

“…We have previously demonstrated that type II ryanodine receptors (RyR2) tetramers can be rapidly rearranged in response to a phosphorylation cocktail. The…”
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Cardiac ryanodine receptor distribution is dynamic and changed by auxiliary proteins and post-translational modification by Asghari, Parisa, Scriven, David Rl, Ng, Myles, Panwar, Pankaj, Chou, Keng C, van Petegem, Filip, Moore, Edwin Dw

“…The effects of the immunophilins, FKBP12 and FKBP12.6, and phosphorylation on type II ryanodine receptor (RyR2) arrangement and function were examined using…”
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Axial Tubules of Rat Ventricular Myocytes Form Multiple Junctions with the Sarcoplasmic Reticulum by Asghari, Parisa, Schulson, Meredith, Scriven, David R.L., Martens, Garnet, Moore, Edwin D.W.

“…Ryanodine receptors (RyRs) are located primarily on the junctional sarcoplasmic reticulum (SR), adjacent to the transverse tubules and on the cell surface near…”
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Multi-Image Colocalization and Its Statistical Significance by Fletcher, Patrick A., Scriven, David R.L., Schulson, Meredith N., Moore, Edwin D.W.

“…Accurately localizing molecules within the cell is one of main tasks of modern biology, and colocalization analysis is one of its principal and most often used…”
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Single molecule localization deep within thick cells; a novel super-resolution microscope by Tafteh, Reza, Scriven, David R. L., Moore, Edwin D. W., Chou, Keng C.

“…A novel 3D imaging system based on single‐molecule localization microscopy is presented to allow high‐accuracy drift‐free (<0.7 nm lateral; 2.5 nm axial)…”
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Image acquisition for colocalization using optical microscopy by Scriven, David R L, Lynch, Ronald M, Moore, Edwin D W

“…Colocalization, in which images of two or more fluorescent markers are overlaid, and coincidence between the probes is measured or displayed, is a common…”
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3D structured illumination microscope using a spinning disk [Invited] by Zhang, Youchang, Asghari, Parisa, Scriven, David R L, Moore, Edwin D W, Chou, Keng C

“…Three-dimensional (3D) structured illumination microscopy (SIM) improves spatial resolution by a factor of two in both lateral and axial directions. However,…”
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Microarchitecture of the dyad by Scriven, David R L, Asghari, Parisa, Moore, Edwin D W

“…This review highlights recent and ongoing discoveries that are transforming the previously held view of dyad structure and function. New data show that dyads…”
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Nonuniform and Variable Arrangements of Ryanodine Receptors Within Mammalian Ventricular Couplons by Asghari, Parisa, Scriven, David R.L, Sanatani, Shubhayan, Gandhi, Sanjiv K, Campbell, Andrew I.M, Moore, Edwin D.W

“…RATIONALE:Single-tilt tomograms of the dyads in rat ventricular myocytes indicated that type 2 ryanodine receptors (RYR2s) were not positioned in a…”
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Ca2+ channel and Na+/Ca2+ exchange localization in cardiac myocytes by Scriven, David R.L., Moore, Edwin D.W.

“…L-type Ca2+ channels and the Na+/Ca2+ exchanger are the main pathways for Ca2+ influx and efflux across the sarcolemma. The majority of Ca2+ channels are found…”
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Uptake of Oxidized LDL by Macrophages Differs From That of Acetyl LDL and Leads to Expansion of an Acidic Endolysosomal Compartment by Lougheed, Marilee, Moore, Edwin D.W, Scriven, David R.L, Steinbrecher, Urs P

“…Accumulation of cholesterol by macrophage foam cells in atherosclerotic lesions is thought to involve the uptake of modified low density lipoproteins (LDLs)…”
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Couplons in rat atria form distinct subgroups defined by their molecular partners by Schulson, Meredith N, Scriven, David R L, Fletcher, Patrick, Moore, Edwin D W

“…Standard local control theory, which describes Ca(2+) release during excitation-contraction coupling (ECC), assumes that all ryanodine receptor 2 (RyR2)…”
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Caveolin-3 Is Adjacent to a Group of Extradyadic Ryanodine Receptors by Scriven, David R.L., Klimek, Agnieszka, Asghari, Parisa, Bellve, Karl, Moore, Edwin D.W.

“…Caveolae are present in almost all cells and concentrate a wide variety of signaling molecules, receptors, transporters, and ion pumps. We have investigated…”
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Epitope-dependent localization of estrogen receptor-alpha, but not -beta, in en face arterial endothelium by Dan, Pauline, Cheung, Joyce C Y, Scriven, David R L, Moore, Edwin D W

“…Rapid, nongenomic effects of 17 beta-estradiol (E(2)) in endothelial cells are postulated to arise from membrane-associated estrogen receptors (ERs), which…”
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The Molecular Architecture of Calcium Microdomains in Rat Cardiomyocytes by SCRIVEN, DAVID R. L., KLIMEK, AGNIESZKA, LEE, KELLY L., MOORE, EDWIN D. W.

“…: We have used standard indirect immunofluorescence techniques in combination with wide‐field microscopy and image deconvolution to assess the distribution of…”
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3D structured illumination microscope using a spinning disk Invited by Zhang, Youchang, Asghari, Parisa, Scriven, David R L, Moore, Edwin D W, Chou, Keng C

“…Three-dimensional (3D) structured illumination microscopy (SIM) improves spatial resolution by a factor of two in both lateral and axial directions. However,…”
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Analysis of Cav1.2 and ryanodine receptor clusters in rat ventricular myocytes by Scriven, David R L, Asghari, Parisa, Schulson, Meredith N, Moore, Edwin D W

“…We analyzed the distribution of ryanodine receptor (RyR) and Cav1.2 clusters in adult rat ventricular myocytes using three-dimensional object-based…”
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RYR2 phosphomutants show that the tetramers and the junctional sarcoplasmic reticulum form a structural syncytium: Calcium Signaling and Excitation–Contraction in Cardiac, Skeletal and Smooth Muscle by Asghari, Parisa, Scriven, David RL, Shahrasebi, Saba, Moore, Edwin DW

“…We previously showed that RYR2 tetramers are distributed nonuniformly within ventricular dyads, and that physiological and pathological factors can alter their…”
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