A 6-Mb High-Resolution Physical and Transcription Map Encompassing the Hereditary Prostate Cancer 1 (HPC1) Region

Several hereditary disease loci have been genetically mapped to the chromosome 1q24–q31 interval, including the hereditary prostate cancer 1 (HPC1) locus. Here, we report the construction of a 20-Mb yeast artificial chromosome contig and a high-resolution 6-Mb sequence-ready bacterial artificial chr...

Full description

Saved in:

Bibliographic Details
Published in:	Genomics (San Diego, Calif.) Vol. 64; no. 1; pp. 1 - 14
Main Authors:	Carpten, John D., Makalowska, Izabella, Robbins, Christiane M., Scott, Nyasha, Sood, Raman, Connors, Tim D., Bonner, Tom I., Smith, Jeffrey R., Faruque, Mezbah U., Stephan, Dietrich A., Pinkett, Heather, Morgenbesser, Sharon D., Su, Kui, Graham, Chris, Gregory, Simon G., Williams, Hawys, McDonald, Louise, Baxevanis, Andreas D., Klingler, Kathy W., Landes, Greg M., Trent, Jeffrey M.
Format:	Journal Article
Language:	English
Published:	San Diego, CA Elsevier Inc 15-02-2000 Elsevier
Subjects:	bacterial artificial chromosomes Base Sequence Biological and medical sciences chromosome 1 Chromosomes, Artificial, Yeast Chromosomes, Human, Pair 1 Classical genetics, quantitative genetics, hybrids Contig Mapping DNA Fingerprinting - methods DNA, Complementary Fundamental and applied biological sciences. Psychology Genetic Predisposition to Disease Genetics of eukaryotes. Biological and molecular evolution Gynecology. Andrology. Obstetrics Human Humans Male Male genital diseases Medical sciences Molecular Sequence Data P1-based artificial chromosomes Physical Chromosome Mapping Polymorphism, Genetic Prostatic Neoplasms - genetics Repetitive Sequences, Nucleic Acid Transcription, Genetic Tumors yeast artificial chromosomes Genetic mapping Human Prostate disease Genetic marker Chromosome A1 Genetic disease Contig Gene Tumor Expressed sequence tag Male genital diseases Prostate Physical map Polymorphism
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Several hereditary disease loci have been genetically mapped to the chromosome 1q24–q31 interval, including the hereditary prostate cancer 1 (HPC1) locus. Here, we report the construction of a 20-Mb yeast artificial chromosome contig and a high-resolution 6-Mb sequence-ready bacterial artificial chromosome (BAC)/P1-derived artificial chromosome (PAC) contig of 1q25 by sequence and computational analysis, STS content mapping, and chromosome walking. One hundred thirty-six new STSs, including 10 novel simple sequence repeat polymorphisms that are being used for genetic refinement of multiple disease loci, have been generated from this contig and are shown to map to the 1q25 interval. The integrity of the 6-Mb BAC/PAC contig has been confirmed by restriction fingerprinting, and this contig is being used as a template for human chromosome 1 genome sequencing. A transcription mapping effort has resulted in the precise localization of 18 known genes and 31 ESTs by database searching, exon trapping, direct cDNA hybridization, and sample sequencing of BACs from the 1q25 contig. An additional 11 known genes and ESTs have been placed within the larger 1q24–q31 interval. These transcription units represent candidate genes for multiple hereditary diseases, including HPC1.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0888-7543 1089-8646
DOI:	10.1006/geno.1999.6051