Search Results - "Scofano, M"

High hydrostatic pressure perturbs the interactions between CF0F1 subunits and induces a dual effect on activity by Souza, Manuela O., Creczynski-Pasa, Tânia B., Scofano, Helena M., Gräber, Peter, Mignaco, Julio A.

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Characterization of Ca2+ uptake in a subcellular membrane fraction of Herpetomonas sp. promastigotes by Sodré, C L, Moreira, B L M, Meyer-Fernandes, J R, Dutra, P M L, Lopes, A H C S, Scofano, H M, Barrabin, H

“…ATP-dependent Ca2+ uptake was studied in a subcellular fraction from Herpetomonas sp. prepared by mechanical disruption and using 45Ca2+ as a tracer. The…”
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Gill microsomal (Na +,K +)-ATPase from the blue crab Callinectes danae: Interactions at cationic sites by Masui, D.C., Furriel, R.P.M., Silva, E.C.C., Mantelatto, F.L.M., McNamara, J.C., Barrabin, H., Scofano, H.M., Fontes, C.F.L., Leone, F.A.

“…Euryhaline crustaceans tolerate exposure to a wide range of dilute media, using compensatory, ion regulatory mechanisms. However, data on molecular…”
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Identification of a crab gill FXYD2 protein and regulation of crab microsomal Na,K-ATPase activity by mammalian FXYD2 peptide by Silva, Elias C.C., Masui, Douglas C., Furriel, Rosa P., McNamara, John C., Barrabin, Hector, Scofano, Helena M., Perales, Jonas, Teixeira-Ferreira, André, Leone, Francisco A., Fontes, Carlos Frederico L.

“…This investigation discloses the recognition of an FXYD2 protein in a microsomal Na,K-ATPase preparation from the posterior gills of the blue crab, Callinectes…”
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A Novel Role of 4,4‘-Diisothiocyanatostilbene-2,2‘-disulfonic Acid as an Activator of the Phosphatase Activity Catalyzed by Plasma Membrane Ca2+-ATPase by Santos, Fernanda T, Scofano, Helena M, Barrabin, Hector, Meyer-Fernandes, José R, Mignaco, Julio A

“…The hydrolysis of p-nitrophenyl phosphate catalyzed by the erythrocyte membrane Ca2+-ATPase is stimulated by low concentrations of the compound…”
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Conformational Changes of the Nucleotide Site of the Plasma Membrane Ca2+-ATPase Probed by Fluorescence Quenching by Fonseca, Mirian M, Scofano, Helena M, Carvalho-Alves, Paulo C, Barrabin, Hector, Mignaco, Julio A

“…Fluorescence quenching by the water-soluble ions I- and Cs+ was used to probe solvent accessibility and polarity of the nucleotide/fluorescein isothiocyanate…”
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Magnesium-dependent ecto-ATP diphosphohydrolase activity in Herpetomonas muscarum muscarum by ALVES-FERREIRA, M, DUTRA, P. M. L, LOPES, A. H. C. S, FERREIRA-PEREIRA, A, SCOFANO, H. M, MEYER-FERNANDES, J. R

“…In the present work we characterized the ecto-ATP diphosphohydrolase activity of the trypanosomatid parasite Herpetomonas muscarum muscarum. This parasite…”
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Stimulation of Ouabain Binding to Na,K-ATPase in 40% Dimethyl Sulfoxide by a Factor from Na,K-ATPase Preparations by Fontes, Carlos F.L., Lopes, Fábio E.Veiga, Scofano, Helena M., Barrabin, Hector, Nørby, Jens G.

“…In 40% dimethyl sulfoxide (Me2SO) high-affinity ouabain (O) binding to Na,K-ATPase (E) is promoted by Mg2+ in the absence of inorganic phosphate (Pi) (Fontes…”
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fully-active and structurally-stable form of the mitochondrial ATP synthase of Polytomella sp. is dimeric by Villavicencio-Queijeiro, Alexa, Vázquez-Acevedo, Miriam, Cano-Estrada, Araceli, Zarco-Zavala, Mariel, Tuena de Gómez, Marietta, Mignaco, Julio A, Freire, Monica M, Scofano, Helena M, Foguel, Debora, Cardol, Pierre, Remacle, Claire, González-Halphen, Diego

“…Mitochondrial F₁FO-ATP synthase of chlorophycean algae is a stable dimeric complex of 1,600 kDa. It lacks the classic subunits that constitute the peripheral…”
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Adenosine triphosphatase site stoichiometry in sarcoplasmic reticulum vesicles and purified enzyme by Barrabin, Hector, Scofano, Helena M, Inesi, Giuseppe

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Converting troponin C into calmodulin: effects of mutations in the central helix and of changes in temperature by da Silva, Elizabeth F., Oliveira, Vanessa H., Sorenson, Martha M., Barrabin, Hector, Scofano, Helena M.

“…Calmodulin (CaM) and troponin C (TnC) are the most similar members of EF-hand family and show few differences in the primary structure. Here, we use mutants of…”
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Protection against thermal denaturation by trehalose on the plasma membrane H+-ATPase from yeast. Synergetic effect between trehalose and phospholipid environment by Felix, C F, Moreira, C C, Oliveira, M S, Sola-Penna, M, Meyer-Fernandes, J R, Scofano, H M, Ferreira-Pereira, A

“…Yeast cells have had to develop mechanisms in order to protect themselves from chemical and physical agents of the environment to which they are exposed. One…”
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The crustacean gill (Na +, K +)-ATPase: Allosteric modulation of high- and low-affinity ATP-binding sites by sodium and potassium by Masui, D.C., Silva, E.C.C., Mantelatto, F.L.M., McNamara, J.C., Barrabin, H., Scofano, H.M., Fontes, C.F.L., Furriel, R.P.M., Leone, F.A.

“…The blue crab, Callinectes danae, tolerates exposure to a wide salinity range employing mechanisms of compensatory ion uptake when in dilute media. Although…”
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Mechanism of modulation of the plasma membrane Ca(2+)-ATPase by arachidonic acid by Oliveira, Vanessa H, Nascimento, Karla S O, Freire, Mônica M, Moreira, Otacilio C, Scofano, Helena M, Barrabin, Hector, Mignaco, Julio A

“…The intracellular level of long chain fatty acids controls the Ca(2+) concentration in the cytoplasm. The molecular mechanisms underlying this Ca(2+)…”
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Regulation by the exogenous polyamine spermidine of Na,K-ATPase activity from the gills of the euryhaline swimming crab Callinectes danae (Brachyura, Portunidae) by Silva, E.C.C., Masui, D.C., Furriel, R.P.M., Mantelatto, F.L.M., McNamara, J.C., Barrabin, H., Leone, F.A., Scofano, H.M., Fontes, C.F.L.

“…Euryhaline crustaceans rarely hyporegulates and employ the driving force of the Na,K-ATPase, located at the basal surface of the gill epithelium, to maintain…”
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The hydrolytic cycle of sarcoplasmic reticulum Ca2+-ATPase in the absence of calcium by Carvalho-Alves, P C, Scofano, H M

“…The hydrolytic cycle of sarcoplasmic reticulum Ca2+-ATPase in the absence of Ca2+ was studied. At pH 6.0, 10 degrees C and in the absence of K+, the enzyme…”
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Inhibition of plasma membrane Ca2+-ATPase by heparin is modulated by potassium by Felix, Carla F., Oliveira, Vanessa H., Moreira, Otacilio C., Mignaco, Julio A., Barrabin, Hector, Scofano, Helena M.

“…Heparin is related to several protein receptors that control Ca2+ homeostasis. Here, we studied the effects of heparin on the plasma membrane Ca2+-ATPase from…”
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Comparison of calmodulin and troponin C with and without its amino-terminal helix (residues 1-11) in the activation of erythrocyte Ca(2+)-ATPase by da Silva, E F, Sorenson, M M, Smillie, L B, Barrabin, H, Scofano, H M

“…Troponin C can replace calmodulin in the activation of the Ca(2+)-ATPase of pig erythrocytes provided that the reaction medium contains relatively high free…”
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Inhibition of spinach chloroplast F0F1 by an Fe2+/ascorbate/H2O2 system by RIBEIRO, Adriana S, SOUZA, Manuela O, SCOFANO, Helena M, CRECZYNSKI-PASA, Tania B, MIGNACO, Julio A

“…Plant chloroplasts are particularly threatened by free radical attack. We incubated purified soluble spinach chloroplast F(0)F(1) (CF(0)F(1), EC 3.6.3.34) with…”
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The role of Mg2+ and K+ in the phosphorylation of Na+,K(+)-ATPase by ATP in the presence of dimethylsulfoxide but in the absence of Na+ by Fontes, C F, Barrabin, H, Scofano, H M, Nørby, J G

“…We have previously demonstrated that Na+,K(+)-ATPase can be phosphorylated by 100 microM ATP and 5 mM Mg2+ and in the absence of Na+, provided that 40%…”
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