WDR5 facilitates recruitment of N-MYC to conserved WDR5 gene targets in neuroblastoma cell lines

Collectively, the MYC family of oncoprotein transcription factors is overexpressed in more than half of all malignancies. The ability of MYC proteins to access chromatin is fundamental to their role in promoting oncogenic gene expression programs in cancer and this function depends on MYC–cofactor i...

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Published in:	Oncogenesis (New York, NY) Vol. 12; no. 1; p. 32
Main Authors:	Bumpous, Leigh A., Moe, Kylie C., Wang, Jing, Carver, Logan A., Williams, Alexandria G., Romer, Alexander S., Scobee, Jesse D., Maxwell, Jack N., Jones, Cheyenne A., Chung, Dai H., Tansey, William P., Liu, Qi, Weissmiller, April M.
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 19-06-2023 Nature Publishing Group
Subjects:	13/106 38/44 45/15 45/70 631/67/2332 631/67/395 Apoptosis Burkitt's lymphoma c-Myc protein Cell Biology Cell cycle Chromatin DNA repair Gene expression Genes Human Genetics Internal Medicine Lymphoma Malignancy Medicine Medicine & Public Health Myc protein Neuroblastoma Oncology Protein biosynthesis Protein synthesis Proteins Transcription factors
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Summary:	Collectively, the MYC family of oncoprotein transcription factors is overexpressed in more than half of all malignancies. The ability of MYC proteins to access chromatin is fundamental to their role in promoting oncogenic gene expression programs in cancer and this function depends on MYC–cofactor interactions. One such cofactor is the chromatin regulator WDR5, which in models of Burkitt lymphoma facilitates recruitment of the c-MYC protein to chromatin at genes associated with protein synthesis, allowing for tumor progression and maintenance. However, beyond Burkitt lymphoma, it is unknown whether these observations extend to other cancers or MYC family members, and whether WDR5 can be deemed as a “universal” MYC recruiter. Here, we focus on N-MYC amplified neuroblastoma to determine the extent of colocalization between N-MYC and WDR5 on chromatin while also demonstrating that like c-MYC, WDR5 can facilitate the recruitment of N-MYC to conserved WDR5-bound genes. We conclude based on this analysis that N-MYC and WDR5 colocalize invariantly across cell lines at predicted sites of facilitated recruitment associated with protein synthesis genes. Surprisingly, we also identify N-MYC-WDR5 cobound genes that are associated with DNA repair and cell cycle processes. Dissection of chromatin binding characteristics for N-MYC and WDR5 at all cobound genes reveals that sites of facilitated recruitment are inherently different than most N-MYC-WDR5 cobound sites. Our data reveals that WDR5 acts as a universal MYC recruiter at a small cohort of previously identified genes and highlights novel biological functions that may be coregulated by N-MYC and WDR5 to sustain the neuroblastoma state.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2157-9024 2157-9024
DOI:	10.1038/s41389-023-00477-z