Search Results - "Schwienhorst, A."

Direct thrombin inhibitors - a survey of recent developments by Schwienhorst, A

“…Thrombin is a plasma serine protease that plays a key role in coagulation and hemostasis but also in thromboembolic diseases. Direct thrombin inhibitors could,…”
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High-fidelity in vitro recombination using a proofreading polymerase by Ninkovic, M, Dietrich, R, Aral, G, Schwienhorst, A

“…We describe a convenient PCR-based protocol for in vitro recombination of homologous genes, thereby minimizing the rate of associated point mutations…”
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Accurate high-speed liquid handling of very small biological samples by Schober, A, Günther, R, Schwienhorst, A, Döring, M, Lindemann, B F

“…Molecular biology techniques require the accurate pipetting of buffers and solutions with volumes in the microliter range. Traditionally, hand-held pipetting…”
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Combinatorial libraries by cassette mutagenesis by Borrego, Belen, Wienecke, Anja, Schwienhorst, Andreas

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Crystal Structure of a Bacterial Class 2 Histone Deacetylase Homologue by Nielsen, Tine Kragh, Hildmann, Christian, Dickmanns, Achim, Schwienhorst, Andreas, Ficner, Ralf

“…Histone deacetylases (HDACs) are among the most promising targets in cancer therapy. However, structural information greatly enhancing the design of HDAC…”
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Rapid selection of aminoacyl-tRNAs based on biotinylation of  -NH2 group of charged amino acids by Putz, J., Wientges, J., Schwienhorst, A., Sissler, M., Giege, R., Florentz, C.

“…A rapid selection procedure to separate low amounts of aminoacylated tRNAs from large pools of inactive variants is described. The procedure involves a…”
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Members of the histone deacetylase superfamily differ in substrate specificity towards small synthetic substrates by Riester, Daniel, Wegener, Dennis, Hildmann, Christian, Schwienhorst, Andreas

“…Histone deacetylases (HDACs) are important enzymes for the transcriptional regulation of gene expression in eukaryotic cells. Deacetylation of ε-acetyl-lysine…”
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Improved fluorogenic histone deacetylase assay for high-throughput-screening applications by Wegener, Dennis, Hildmann, Christian, Riester, Daniel, Schwienhorst, Andreas

“…Histone deacetylases (HDACs) are key targets for chemotherapeutic intervention in malignant diseases. In this paper, a highly sensitive, nonisotopic,…”
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Factors affecting the substrate specificity of histone deacetylases by Riester, Daniel, Hildmann, Christian, Grünewald, Sylvia, Beckers, Thomas, Schwienhorst, Andreas

“…Histone deacetylases (HDACs) catalyze the deacetylation of ε-acetyl-lysine residues within the N-terminal tail of core histones and thereby mediate changes in…”
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Hamming chromatography by Schwienhorst, A, Schober, A, Günther, R, Stadler, P F

“…Selection of molecules with desired properties from random pools of biopolymers has become a powerful tool in biotechnology. On designing an evolution…”
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HKI 46F08, a novel potent histone deacetylase inhibitor, exhibits antitumoral activity against embryonic childhood cancer cells by Wegener, Dennis, Deubzer, Hedwig E, Oehme, Ina, Milde, Till, Hildmann, Christian, Schwienhorst, Andreas, Witt, Olaf

“…Embryonic childhood cancer such as neuroblastoma and medulloblastoma are still a therapeutic challenge requiring novel treatment approaches. Here, we…”
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Recent progress in the development of assays suited for histone deacetylase inhibitor screening by Wegener, Dennis, Hildmann, Christian, Schwienhorst, Andreas

“…Histone deacetylase (HDAC) inhibitors have an unprecedented potential to occupy a major position in the future market of anticancer agents. However, progress…”
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A New Amidohydrolase from Bordetella or Alcaligenes Strain FB188 with Similarities to Histone Deacetylases by HILDMANN, Christian, NINKOVIC, Milena, DIETRICH, Rüdiger, WEGENER, Dennis, RIESTER, Daniel, ZIMMERMANN, Thomas, BIRCH, Olwen M, BERNEGGER, Christine, LOIDL, Peter, SCHWIENHORST, Andreas

“…Article Usage Stats Services JB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
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Thrombin Inhibitors Identified by Computer-Assisted Multiparameter Design by Riester, Daniel, Wirsching, Frank, Salinas, Gabriela, Keller, Martina, Gebinoga, Michael, Kamphausen, Stefan, Merkwirth, Christian, Goetz, Ruediger, Wiesenfeldt, Martin, Stürzebecher, Jörg, Bode, Wolfram, Friedrich, Rainer, Thürk, Marcel, Schwienhorst, Andreas, Eigen, Manfred

“…Here, we present a series of thrombin inhibitors that were generated by using powerful computer-assisted multiparameter optimization process. The process was…”
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Genetic algorithm for the design of molecules with desired properties by Kamphausen, Stefan, Höltge, Nils, Wirsching, Frank, Morys-Wortmann, Corinna, Riester, Daniel, Goetz, Ruediger, Thürk, Marcel, Schwienhorst, Andreas

“…The design of molecules with desired properties is still a challenge because of the largely unpredictable end results. Computational methods can be used to…”
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Directed evolution towards protease-resistant hirudin variants by Wirsching, Frank, Keller, Martina, Hildmann, Christian, Riester, Daniel, Schwienhorst, Andreas

“…Hirudin, a thrombin-specific inhibitor, is efficiently digested and inactivated by proteases with pepsin- and chymotrypsin-like specificity. Using a…”
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A New Reporter Gene System Suited for Cell-Free Protein Synthesis and High-Throughput Screening in Small Reaction Volumes by Hempel, René, Wirsching, Frank, Schober, Andreas, Schwienhorst, Andreas

“…The properties of M-hirudin as a new reporter gene system were examined using rabbit reticulocyte lysate for cell-free protein expression. In contrast to the…”
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Modular Design of a Novel Chimeric Protein with Combined Thrombin Inhibitory Activity and Plasminogen-Activating Potential by Wirsching, Frank, Luge, Cornelia, Schwienhorst, Andreas

“…In order to design plasminogen activators with improved thrombolytic properties we sought to construct the bifunctional protein HLS-2 which combines both a…”
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Fluorogenic Assay for Penicillin G Acylase Activity by Ninkovic, Milena, Riester, Daniel, Wirsching, Frank, Dietrich, Rüdiger, Schwienhorst, Andreas

“…A simple, highly sensitive, and rapid assay for high-throughput screening of penicillin G acylase-producing bacteria is presented. The method is based on the…”
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Evolution of Bacteriophage in Continuous Culture: a Model System To Test Antiviral Gene Therapies for the Emergence of Phage Escape Mutants by Lindemann, Björn F, Klug, Christian, Schwienhorst, Andreas

“…Article Usage Stats Services JVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
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