Search Results - "Schwark, David G"

Directed Evolution of the Methanosarcina barkeri Pyrrolysyl tRNA/aminoacyl tRNA Synthetase Pair for Rapid Evaluation of Sense Codon Reassignment Potential by Schwark, David G, Schmitt, Margaret A, Fisk, John D

“…Genetic code expansion has largely focused on the reassignment of amber stop codons to insert single copies of non-canonical amino acids (ncAAs) into proteins…”
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The Influence of Competing tRNA Abundance on Translation: Quantifying the Efficiency of Sense Codon Reassignment at Rarely Used Codons by Schwark, David G., Schmitt, Margaret A., Biddle, Wil, Fisk, John D.

“…A quantitative understanding of how system composition and molecular properties conspire to determine the fidelity of translation is lacking. Our strategy…”
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Directed Evolution Pipeline for the Improvement of Orthogonal Translation Machinery for Genetic Code Expansion at Sense Codons by Biddle, Wil, Schwark, David G, Schmitt, Margaret A, Fisk, John D

“…The expansion of the genetic code beyond a single type of noncanonical amino acid (ncAA) is hindered by inefficient machinery for reassigning the meaning of…”
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Impact of queuosine modification of endogenous E. coli tRNAs on sense codon reassignment by Tittle, Jillyn M., Schwark, David G., Biddle, Wil, Schmitt, Margaret A., Fisk, John D.

“…The extent to which alteration of endogenous tRNA modifications may be exploited to improve genetic code expansion efforts has not been broadly investigated…”
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Dissecting the Contribution of Release Factor Interactions to Amber Stop Codon Reassignment Efficiencies of the Methanocaldococcus jannaschii Orthogonal Pair by Schwark, David G, Schmitt, Margaret A, Fisk, John D

“…Non-canonical amino acids (ncAAs) are finding increasing use in basic biochemical studies and biomedical applications. The efficiency of ncAA incorporation is…”
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