Investigation of DNA topoisomerase I as an autoantigen at the thyroid cancer

The data concerning DNA-Topoisomerase I (Topo-I) from human placenta as an autoantigen at the thyroid cancer are presented. A new effective scheme of the Topo-I purification using the High-Trap columns is described, as well as the enzyme physico-chemical properties and its immunoreactivity in the EL...

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Bibliographic Details
Published in:	Biopolimery i kletka Vol. 17; no. 6; pp. 551 - 555
Main Authors:	Sidorik, L. L., Rybkinska, T. A., Vigontina, O. G., Vudmaska, M. I., Naidenov, V. G., Pogribnyi, P. V., Lisovskii, I. L., Schvachko, L. P., Bronstein, I., Lyzogubov, V. V., Prihod'ko, E. A., Usenko, V. S., Matsuka, G. Kh
Format:	Journal Article
Language:	English
Published:	Kiev Natsional'na Akademiya Nauk Ukrainy - National Academy of Sciences of Ukraine 2001
Subjects:	Autoantibodies Deoxyribonucleic acid DNA DNA topoisomerase Immunoblotting Immunoreactivity Physicochemical properties Placenta Purification Thyroid cancer Thyroiditis
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Summary:	The data concerning DNA-Topoisomerase I (Topo-I) from human placenta as an autoantigen at the thyroid cancer are presented. A new effective scheme of the Topo-I purification using the High-Trap columns is described, as well as the enzyme physico-chemical properties and its immunoreactivity in the ELJSA and immunoblotting. It was shown that 46 % of the sera from patients suffering the thyroid cancer were immunoreactive against Topo-I, 36 % of autoantibodies from thyroid cancer patients sera recognized sequential determinants on the Topo-I molecule. The highest levels of immunoreacivity were observed in the sera of thyroid cancer patients accompanied by autoimmune thyroiditis. We suggest a possible linkage between the autoimmune processes and malignization of the thyroid gland cells.
ISSN:	0233-7657 1993-6842
DOI:	10.7124/bc.0005DF