Search Results - "Schulenberg, B"

Analysis of Steady-state Protein Phosphorylation in Mitochondria Using a Novel Fluorescent Phosphosensor Dye by Schulenberg, Birte, Aggeler, Robert, Beechem, Joseph M., Capaldi, Roderick A., Patton, Wayne F.

“…The phosphorylation of mitochondrial proteins is pivotal to the regulation of respiratory activity in the cell and to signaling pathways leading to apoptosis,…”
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Global quantitative phosphoprotein analysis using Multiplexed Proteomics technology by Steinberg, Thomas H., Agnew, Brian J., Gee, Kyle R., Leung, Wai-Yee, Goodman, Terrie, Schulenberg, Birte, Hendrickson, Jill, Beechem, Joseph M., Haugland, Richard P., Patton, Wayne F.

“…Systematic parallel analysis of the phosphorylation status of networks of interacting proteins involved in the regulatory circuitry of cells and tissues is…”
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Characterization of dynamic and steady-state protein phosphorylation using a fluorescent phosphoprotein gel stain and mass spectrometry by Schulenberg, Birte, Goodman, Terrie N., Aggeler, Robert, Capaldi, Roderick A., Patton, Wayne F.

“…Protein phosphorylation plays a vital role in the regulation of most aspects of cellular activity, being key to propagating messages within signal transduction…”
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Two-dimensional gel electrophoresis; better than a poke in the ICAT? by Patton, Wayne F, Schulenberg, Birte, Steinberg, Thomas H

“…To date, the most widely used technology for conducting proteomic studies has been two-dimensional gel electrophoresis (2DGE), but this approach does have…”
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Mass spectrometric identification of a novel phosphorylation site in subunit NDUFA10 of bovine mitochondrial complex I by Schilling, Birgit, Aggeler, Robert, Schulenberg, Birte, Murray, James, Row, Richard H., Capaldi, Roderick A., Gibson, Bradford W.

“…Mitochondrial Complex I (NADH:ubiquinone oxidoreductase) consists of at least 46 subunits. Phosphorylation of the 42-kDa subunit NDUFA10 was recently reported…”
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An improved formulation of SYPRO Ruby protein gel stain: Comparison with the original formulation and with a ruthenium II tris (bathophenanthroline disulfonate) formulation by Berggren, Kiera N., Schulenberg, Birte, Lopez, Mary F., Steinberg, Thomas H., Bogdanova, Alla, Smejkal, Gary, Wang, Annie, Patton, Wayne F.

“…SYPRO Ruby protein gel stain is compatible with a variety of imaging platforms since it absorbs maximally in the ultraviolet (280 nm) and visible (470 nm)…”
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Detection of glycoproteins in polyacrylamide gels and on electroblots using Pro-Q Emerald 488 dye, a fluorescent periodate Schiff-base stain by Hart, Courtenay, Schulenberg, Birte, Steinberg, Thomas H., Leung, Wai-Yee, Patton, Wayne F.

“…Pro‐Q Emerald 488 glycoprotein stain reacts with periodic acid‐oxidized carbohydrate groups, generating a bright green‐fluorescent signal on glycoproteins. The…”
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A novel subfractionation approach for mitochondrial proteins: A three-dimensional mitochondrial proteome map by Hanson, Bonnie J., Schulenberg, Birte, Patton, Wayne F., Capaldi, Roderick A.

“…As mitochondria play critical roles in both cell life and cell death, there is great interest in obtaining a human mitochondrial proteome map. Such a map could…”
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Combining microscale solution-phase isoelectric focusing with Multiplexed Proteomics® dye staining to analyze protein post-translational modifications by Schulenberg, Birte, Patton, Wayne F.

“…Previously, a strategy for rapidly identifying mitochondrial phosphoproteins was presented that involves prefractionating multisubunit complexes by sucrose…”
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Detection of phosphoproteins on electroblot membranes using a small-molecule organic fluorophore by Goodman, Teresa, Schulenberg, Birte, Steinberg, Thomas H., Patton, Wayne F.

“…A new formulation of the small‐molecule organic fluorophore, Pro‐Q® Diamond dye, has been developed that permits rapid and simple detection of phosphoproteins…”
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Mapping glycosylation changes related to cancer using the Multiplexed Proteomics technology: a protein differential display approach by Schulenberg, Birte, Beechem, Joseph M., Patton, Wayne F.

“…The metastatic spread of tumor cells in malignant progression is known to be a major cause of cancer mortality. Protein glycosylation is increasingly being…”
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Optimized conditions for diluting and reusing a fluorescent protein gel stain by Ahnert, Nancy, Patton, Wayne F., Schulenberg, Birte

“…This study elucidates the optimum conditions at the minimum cost for using SYPRO Ruby protein gel stain. It deals with the effects of gel fixation and staining…”
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Cross-linking and electron microscopy studies of the structure and functioning of the Escherichia coli ATP synthase by Capaldi, R A, Schulenberg, B, Murray, J, Aggeler, R

“…ATP synthase, also called F(1)F(o)-ATPase, catalyzes the synthesis of ATP during oxidative phosphorylation. The enzyme is reversible and is able to use ATP to…”
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The gamma epsilon -c Subunit Interface in the ATP Synthase of Escherichia coli: Cross-linking of the epsilon Subunit to the c Subunit Ring Does Not Impair Enzyme Function, That of gamma to c Subunits Leads to Uncoupling by Schulenberg, B, Aggeler, R, Murray, J, Capaldi, R A

“…Mutants with a cysteine residue in the gamma subunit at position 207 and the epsilon subunit at position 31 were expressed in combination with a c-dimer…”
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The epsilon Subunit of the F sub(1)F sub(0) Complex of Escherichia coli Cross-linking Studies Show the Same Structure in situ as when isolated by Schulenberg, B, Capaldi, R A

“…Four double mutants in the epsilon subunit were generated, each containing two cysteines, which, based on the NMR structure of this subunit, should form…”
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The epsilon subunit of the F(1)F(0) complex of Escherichia coli. cross-linking studies show the same structure in situ as when isolated by Schulenberg, B, Capaldi, R A

“…Four double mutants in the epsilon subunit were generated, each containing two cysteines, which, based on the NMR structure of this subunit, should form…”
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Selective proteome-wide detection of hydrophobic integral membrane proteins using a novel fluorescence-based staining technology by Hart, Courtenay, Schulenberg, Birte, Patton, Wayne F.

“…Integral proteins containing two or more α‐helical membrane‐spanning domains are underrepresented in two‐dimensional gels. While sodium dodecyl sulfate…”
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An improved mechanically durable electrophoresis gel matrix that is fully compatible with fluorescence-based protein detection technologies by Schulenberg, Birte, Arnold, Brad, Patton, Wayne F.

“…Unfortunately, conventional large‐format polyacrylamide gels are mechanically fragile, often tearing during the subsequent manipulations required for…”
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Fluorescence detection and quantitation of recombinant proteins containing oligohistidine tag sequences directly in sodium dodecyl sulfate-polyacrylamide gels by Hart, Courtenay, Schulenberg, Birte, Diwu, Zhenjun, Leung, Wai-Yee, Patton, Wayne F.

“…Two fluorophore‐nitrilotriacetic acid conjugates, Pro‐Q Sapphire 365 and Pro‐Q Sapphire 488 oligohistidine gel stains, have been developed for the fluorescence…”
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The gammaepsilon-c subunit interface in the ATP synthase of Escherichia coli. cross-linking of the epsilon subunit to the c subunit ring does not impair enzyme function, that of gamma to c subunits leads to uncoupling by Schulenberg, B, Aggeler, R, Murray, J, Capaldi, R A

“…Mutants with a cysteine residue in the gamma subunit at position 207 and the epsilon subunit at position 31 were expressed in combination with a c-dimer…”
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