Dental phosphoprotein-induced formation of hydroxylapatite during in vitro synthesis of amorphous calcium phosphate

(Ethylenedinitrilo)tetraacetic acid soluble phosphoproteins were isolated from rat incisor and bovine unerupted teeth. This material was examined for its effect on the stability of amorphous calcium phosphate in vitro. When the precipitation of amorphous calcium phosphate was attempted in the presen...

Full description

Saved in:

Bibliographic Details
Published in:	Biochemistry (Easton) Vol. 15; no. 16; pp. 3445 - 3449
Main Authors:	Nawrot, Charles F, Campbell, Darlene J, Schroeder, Jeanne K, Van Valkenburg, Michael
Format:	Journal Article
Language:	English
Published:	United States American Chemical Society 01-08-1976
Subjects:	Amino Acids - analysis Animals Binding Sites Calcium Phosphates - metabolism Cattle Dentin - metabolism Hydroxyapatites - metabolism Incisor - metabolism Male Molecular Conformation Phosphoproteins - metabolism Rats Spectrophotometry, Infrared Tooth, Unerupted - metabolism X-Ray Diffraction
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	(Ethylenedinitrilo)tetraacetic acid soluble phosphoproteins were isolated from rat incisor and bovine unerupted teeth. This material was examined for its effect on the stability of amorphous calcium phosphate in vitro. When the precipitation of amorphous calcium phosphate was attempted in the presence of small amounts of these phospho-proteins, an apatite-like mineral was observed to form, which was approximately 60% crystalline, as determined by infrared measurements. This apatite phase could not be induced by addition of phosphoprotein after the precipitation reaction. The organic phosphate bound to these phosphoproteins was shown to be directly responsible for the formation of the apatite phase, since removal of 60% of the covalently bound phosphate with alkaline phosphatase destroyed the protein's ability to induce hydroxylapatite formation. The properties of the dental phosphoproteins appear to be consistent with their possible involvement in the development of the mineral phase of dentine.
Bibliography:	ark:/67375/TPS-VJ71CSX6-9 istex:A92E52D51D6A96AEF2FA60381B590B80291986C3 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0006-2960 1520-4995
DOI:	10.1021/bi00661a008