Search Results - "Schramm, Vern L."

Enzymatic Transition States, Transition-State Analogs, Dynamics, Thermodynamics, and Lifetimes by Schramm, Vern L

“…Experimental analysis of enzymatic transition-state structures uses kinetic isotope effects (KIEs) to report on bonding and geometry differences between…”
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Enzymatic transition states and dynamic motion in barrier crossing by Schramm, Vern L, Schwartz, Steven D

“…What are the atomic motions at enzymatic catalytic sites on the timescale of chemical change? Combined experimental and computational chemistry approaches take…”
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Human DNMT1 transition state structure by Du, Quan, Wang, Zhen, Schramm, Vern L.

“…Human DNA methyltransferase 1 (DNMT1) maintains the epigenetic state of DNA by replicating CpG methylation signatures from parent to daughter strands,…”
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Purine and pyrimidine pathways as targets in Plasmodium falciparum by Cassera, María Belén, Zhang, Yong, Hazleton, Keith Z, Schramm, Vern L

“…Malaria is a leading cause of morbidity and mortality in the tropics. Chemotherapeutic and vector control strategies have been applied for more than a century…”
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Femtosecond dynamics coupled to chemical barrier crossing in a Born-Oppenheimer enzyme by Silva, Rafael G, Murkin, Andrew S, Schramm, Vern L

“…Contributions of fast (femtosecond) dynamic motion to barrier crossing at enzyme catalytic sites is in dispute. Human purine nucleoside phosphorylase (PNP)…”
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Isotope-specific and amino acid-specific heavy atom substitutions alter barrier crossing in human purine nucleoside phosphorylase by Suarez, Javier, Schramm, Vern L.

“…Computational chemistry predicts that atomic motions on the femtosecond timescale are coupled to transition-state formation (barrier-crossing) in human purine…”
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The biochemistry of sirtuins by Sauve, Anthony A, Wolberger, Cynthia, Schramm, Vern L, Boeke, Jef D

“…Sirtuins are a family of NAD+-dependent protein deacetylases widely distributed in all phyla of life. Accumulating evidence indicates that sirtuins are…”
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Inhibition of Clostridium difficile TcdA and TcdB toxins with transition state analogues by Paparella, Ashleigh S., Aboulache, Briana L., Harijan, Rajesh K., Potts, Kathryn S., Tyler, Peter C., Schramm, Vern L.

“…Clostridium difficile causes life-threatening diarrhea and is the leading cause of healthcare-associated bacterial infections in the United States. TcdA and…”
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Weak Coupling of ATP Hydrolysis to the Chemical Equilibrium of Human Nicotinamide Phosphoribosyltransferase by Burgos, Emmanuel S, Schramm, Vern L

“…Human nicotinamide phosphoribosyltransferase (NAMPT, EC 2.4.2.12) catalyzes the reversible synthesis of nicotinamide mononucleotide (NMN) and inorganic…”
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Transition state analogue of MTAP extends lifespan of APCMin/+ mice by Firestone, Ross S., Feng, Mu, Basu, Indranil, Peregrina, Karina, Augenlicht, Leonard H., Schramm, Vern L.

“…A mouse model of human Familial Adenomatous Polyposis responds favorably to pharmacological inhibition of 5′-methylthioadenosine phosphorylase (MTAP)…”
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Detecting Ricin: Sensitive Luminescent Assay for Ricin A-Chain Ribosome Depurination Kinetics by Sturm, Matthew B, Schramm, Vern L

“…Ricin is a family member of the lethal ribosome-inactivating proteins (RIP) found in plants. Ricin toxin A-chain (RTA) from castor beans catalyzes the…”
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Transition state for the NSD2-catalyzed methylation of histone H3 lysine 36 by Poulin, Myles B., Schneck, Jessica L., Matico, Rosalie E., McDevitt, Patrick J., Huddleston, Michael J., Hou, Wangfang, Johnson, Neil W., Thrall, Sara H., Meek, Thomas D., Schramm, Vern L.

“…Nuclear receptor SET domain containing protein 2 (NSD2) catalyzes the methylation of histone H3 lysine 36 (H3K36). It is a determinant in Wolf–Hirschhorn…”
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Electrophilic Aromatic Selenylation: New OPRT Inhibitors by Abdo, Mohannad, Zhang, Yong, Schramm, Vern L, Knapp, Spencer

“…2-Ethoxyethaneseleninic acid reacts with electron-rich aromatic substrates to deliver, by way of the selenoxides, the (2-ethoxyethyl)seleno ethers, which can…”
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Enzymatic Transition States and Drug Design by Schramm, Vern L

“…Transition state theory teaches that chemically stable mimics of enzymatic transition states will bind tightly to their cognate enzymes. Kinetic isotope…”
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Transition state analogs of 5′-methylthioadenosine nucleosidase disrupt quorum sensing by Schramm, Vern L, Gutierrez, Jemy A, Crowder, Tamara, Rinaldo-Matthis, Agnes, Ho, Meng-Chiao, Almo, Steven C

“…5′-Methylthioadenosine/ S -adenosylhomocysteine nucleosidase (MTAN) is a bacterial enzyme involved in S -adenosylmethionine–related quorum sensing pathways…”
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Sir2 Regulation by Nicotinamide Results from Switching between Base Exchange and Deacetylation Chemistry by Sauve, Anthony A, Schramm, Vern L

“…Life span regulation and inhibition of gene silencing in yeast have been linked to nicotinamide effects on Sir2 enzymes. The Sir2 enzymes are NAD+-dependent…”
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Atomic detail of chemical transformation at the transition state of an enzymatic reaction by Saen-oon, Suwipa, Quaytman-Machleder, Sara, Schramm, Vern L, Schwartz, Steven D

“…Transition path sampling (TPS) has been applied to the chemical step of human purine nucleoside phosphorylase (PNP). The transition path ensemble provides…”
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Ground-State Destabilization in Orotate Phosphoribosyltransferases by Binding Isotope Effects by Zhang, Yong, Schramm, Vern L

“…Orotate phosphoribosyltransferases (OPRTs) form and break the N-ribosidic bond to pyrimidines by way of ribocation-like transition states (TSs) and therefore…”
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Transition state analogues in structures of ricin and saporin ribosome-inactivating proteins by Ho, Meng-Chiao, Sturm, Matthew B, Almo, Steven C, Schramm, Vern L

“…Ricin A-chain (RTA) and saporin-L1 (SAP) catalyze adenosine depurination of 28S rRNA to inhibit protein synthesis and cause cell death. We present the crystal…”
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The Immucillins: Design, Synthesis and Application of Transition- State Analogues by Evans, Gary B, Schramm, Vern L, Tyler, Peter C

“…Transition-state analysis based on kinetic isotope effects and computational chemistry provides electrostatic potential maps to serve as blueprints for the…”
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