Search Results - "Schramm, Thorben"

Deciphering the physiological response of Escherichia coli under high ATP demand by Boecker, Simon, Slaviero, Giulia, Schramm, Thorben, Szymanski, Witold, Steuer, Ralf, Link, Hannes, Klamt, Steffen

“…One long‐standing question in microbiology is how microbes buffer perturbations in energy metabolism. In this study, we systematically analyzed the impact of…”
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Mapping temperature‐sensitive mutations at a genome scale to engineer growth switches in Escherichia coli by Schramm, Thorben, Lubrano, Paul, Pahl, Vanessa, Stadelmann, Amelie, Verhülsdonk, Andreas, Link, Hannes

“…Abstract Temperature‐sensitive (TS) mutants are a unique tool to perturb and engineer cellular systems. Here, we constructed a CRISPR library with 15,120…”
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Von der Stöchiometrie zur Kontrolle metabolischer Netzwerke by Schramm, Thorben, Link, Hannes

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Time-Optimized Isotope Ratio LC–MS/MS for High-Throughput Quantification of Primary Metabolites by Guder, Jan Christopher, Schramm, Thorben, Sander, Timur, Link, Hannes

“…Cellular metabolite concentrations hold information on the function and regulation of metabolic networks. However, current methods to measure metabolites are…”
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Broadening the Scope of Enforced ATP Wasting as a Tool for Metabolic Engineering in Escherichia coli by Boecker, Simon, Zahoor, Ahmed, Schramm, Thorben, Link, Hannes, Klamt, Steffen

“…The targeted increase of cellular adenosine triphosphate (ATP) turnover (enforced ATP wasting) has recently been recognized as a promising tool for metabolic…”
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Von der Stöchiometrie zur Kontrolle metabolischer Netzwerke by Schramm, Thorben, Link, Hannes

“…Abstract Cellular metabolism is very complex and extensively regulated. For many organisms we know almost the complete set of biochemical reactions in their…”
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Systematic analysis of in-source modifications of primary metabolites during flow-injection time-of-flight mass spectrometry by Farke, Niklas, Schramm, Thorben, Verhülsdonk, Andreas, Rapp, Johanna, Link, Hannes

“…Flow-injection mass spectrometry (FI-MS) enables metabolomics studies with a very high sample-throughput. However, FI-MS is prone to in-source modifications of…”
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DarA-the central processing unit for the integration of osmotic with potassium and amino acid homeostasis in Bacillus subtilis by Warneke, Robert, Herzberg, Christina, Weiß, Martin, Schramm, Thorben, Hertel, Dietrich, Link, Hannes, Stülke, Jörg

“…Cyclic di-adenosine monophosphate (c-di-AMP) is a second messenger involved in diverse metabolic processes including osmolyte uptake, cell wall homeostasis, as…”
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How To Deal with Toxic Amino Acids: the Bipartite AzlCD Complex Exports Histidine in Bacillus subtilis by Meißner, Janek, Schramm, Thorben, Hoßbach, Ben, Stark, Katharina, Link, Hannes, Stülke, Jörg

“…The Gram-positive model bacterium Bacillus subtilis can use several amino acids as sources of carbon and nitrogen. However, some amino acids inhibit the growth…”
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High-throughput enrichment of temperature-sensitive argininosuccinate synthetase for two-stage citrulline production in E. coli by Schramm, Thorben, Lempp, Martin, Beuter, Dominik, Sierra, Silvia González, Glatter, Timo, Link, Hannes

“…Controlling metabolism of engineered microbes is important to modulate cell growth and production during a bioprocess. For example, external parameters such as…”
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C4‐dicarboxylates and l‐aspartate utilization by Escherichia coli K‐12 in the mouse intestine: l‐aspartate as a major substrate for fumarate respiration and as a nitrogen source by Schubert, Christopher, Winter, Maria, Ebert‐Jung, Andrea, Kierszniowska, Sylwia, Nagel‐Wolfrum, Kerstin, Schramm, Thorben, Link, Hannes, Winter, Sebastian, Unden, Gottfried

“…Summary C4‐dicarboxylates, such as fumarate, l‐malate and l‐aspartate represent substrates for anaerobic growth of Escherichia coli by fumarate respiration…”
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