Search Results - "Schrag, J D"

Insights into interfacial activation from an open structure of Candida rugosa lipase by Grochulski, P, Li, Y, Schrag, J.D, Bouthillier, F, Smith, P, Harrison, D, Rubin, B, Cygler, M

“…The structure of the Candida rugosa lipase determined at 2.06-angstroms resolution reveals a conformation with a solvent-accessible active site. Comparison…”
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Interaction of a G-protein β-subunit with a conserved sequence in Ste20/PAK family protein kinases by Leberer, Ekkehard, Leeuw, Thomas, Wu, Cunle, Schrag, Joseph D, Whiteway, Malcolm, Thomas, David Y

“…Serine/threonine protein kinases of the Ste20/PAK family have been implicated in the signalling from heterotrimeric G proteins to mitogen-activated protein…”
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Ser-His-Glu triad forms the catalytic site of the lipase from Geotrichum candidum by Schrag, J.D, Li, Y, Wu, S, Cygler, M

“…The Ser-His-Asp triad is a well known structural feature of the serine proteases. It has also been directly observed in the catalytic sites of two lipases,…”
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The Structure of Calnexin, an ER Chaperone Involved in Quality Control of Protein Folding by Schrag, Joseph D., Bergeron, John J.M., Li, Yunge, Borisova, Svetlana, Hahn, Michael, Thomas, David Y., Cygler, Miroslaw

“…The three-dimensional structure of the lumenal domain of the lectin-like chaperone calnexin determined to 2.9 Å resolution reveals an extended 140 Å arm…”
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Pancreatic lipases: evolutionary intermediates in a positional change of catalytic carboxylates? by SCHRAG, J. D, WINKLER, F. K, CYGLER, M

“…Comparison of the fold of lipases from Geotrichum candidum and from human pancreas identified a high degree of similarity which was not expected on the basis…”
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Two conformational states of Candida rugosa lipase by Grochulski, Pawel, Li, Yunge, Schrag, Joseph D., Cygler, Miroslaw

“…The structure of Candida rugosa lipase in a new crystal form has been determined and refined at 2.1 Å resolution. The lipase molecule was found in an inactive…”
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Relationship between sequence conservation and three‐dimensional structure in a large family of esterases, lipases, and related proteins by Cygler, Miroslaw, Schrag, Joseph D., Sussman, Joel L., Harel, Michal, Silman, Israel, Gentry, Mary K., Doctor, Bhupendra P.

“…Based on the recently determined X‐ray structures of Torpedo californica acetylcholinesterase and Geotrichum candidum lipase and on their three‐dimensional…”
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Three-Dimensional Structure of 2-Amino-3-ketobutyrate CoA Ligase from Escherichia coli Complexed with a PLP−Substrate Intermediate:  Inferred Reaction Mechanism by Schmidt, Andrea, Sivaraman, J, Li, Yunge, Larocque, Robert, Barbosa, João A. R. G, Smith, Christopher, Matte, Allan, Schrag, Joseph D, Cygler, Miroslaw

“…2-Amino-3-ketobutyrate CoA ligase (KBL, EC 2.3.1.29) is a pyridoxal phosphate (PLP) dependent enzyme, which catalyzes the second reaction step on the main…”
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Structure and conformational flexibility of Candida rugosa lipase by Cygler, Miroslaw, Schrag, Joseph D

“…Three-dimensional structures of a number of lipases determined in the past decade have provided a solid structural foundation for our understanding of lipase…”
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Structural determinants defining common stereoselectivity of lipases toward secondary alcohols by Cygler, M, Grochulski, P, Schrag, J D

“…In this review we summarize some aspects of the enantiopreference of the lipase from Candida rugosa following structural analysis of complexes of this lipase…”
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1.8 A refined structure of the lipase from Geotrichum candidum by Schrag, J D, Cygler, M

“…A lipase from the fungus Geotrichum candidum is one of only three interfacially activated lipases whose structures have been reported to date. We have…”
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Structure of an aryl esterase from Pseudomonas fluorescens by Cheeseman, Jeremy D., Tocilj, Ante, Park, Seongsoon, Schrag, Joseph D., Kazlauskas, Romas J.

“…The structure of PFE, an aryl esterase from Pseudomonas fluorescens, has been solved to a resolution of 1.8 Å by X‐ray diffraction and shows a characteristic…”
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Cloning and expression of Geotrichum candidum lipase II gene in yeast. Probing of the enzyme active site by site-directed mutagenesis by Vernet, T, Ziomek, E, Recktenwald, A, Schrag, J D, de Montigny, C, Tessier, D C, Thomas, D Y, Cygler, M

“…The three-dimensional structure of lipase II of Geotrichum candidum strain ATCC34614 (GCL II) has provided insights with respect to the nature of the catalytic…”
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The open conformation of a Pseudomonas lipase by Schrag, Joseph D, Li, Yunge, Cygler, Miroslaw, Lang, Dietmar, Burgdorf, Tanja, Hecht, Hans-Juergen, Schmid, Rolf, Schomburg, Dietmar, Rydel, Timothy J, Oliver, Joel D, Strickland, Larry C, Dunaway, C Michelle, Larson, Steven B, Day, John, McPherson, Alexander

“…Background: The interfacial activation of lipases results primarily from conformational changes in the enzymes which expose the active site and provide a…”
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Structure of the 16S rRNA pseudouridine synthase RsuA bound to uracil and UMP by Cygler, Miroslaw, Matte, Allan, Sivaraman, J, Sauvé, Véronique, Larocque, Robert, Stura, Enrico A, Schrag, Joseph D

“…In Escherichia coli, the pseudouridine synthase RsuA catalyzes formation of pseudouridine (ψ) at position 516 in 16S rRNA during assembly of the 30S ribosomal…”
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Crystal Structure of Methanobacterium thermoautotrophicum Phosphoribosyl-AMP Cyclohydrolase HisI by Sivaraman, J, Myers, Rebecca S, Boju, Lorena, Sulea, Traian, Cygler, Miroslaw, Davisson, V. Jo, Schrag, Joseph D

“…The metabolic pathway for histidine biosynthesis is interesting from an evolutionary perspective because of the diversity of gene organizations and protein…”
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Crystallization and preliminary X-ray diffraction analysis of 11 S acetylcholinesterase by Schrag, J D, Schmid, M F, Morgan, D G, Phillips, G N, Chiu, W, Tang, L

“…The 11 S form of acetylcholinesterase from Electrophorus electricus was purified by affinity chromatography. The protein was crystallized from polyethylene…”
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Analogs of Reaction Intermediates Identify a Unique Substrate Binding Site in Candida rugosa Lipase by Grochulski, Pawel, Bouthillier, Francois, Kazlauskas, Romas J, Serreqi, Alessio N, Schrag, Joseph D, Ziomek, Edmund, Cygler, Miroslaw

“…The structures of Candida rugosa lipase-inhibitor complexes demonstrate that the scissile fatty acyl chain is bound in a narrow, hydrophobic tunnel which is…”
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Crystal structure of histidinol phosphate aminotransferase (HisC) from Escherichia coli, and its covalent complex with pyridoxal-5′-phosphate and l-histidinol phosphate by Sivaraman, J, Li, Yunge, Larocque, Robert, Schrag, Joseph D, Cygler, Miroslaw, Matte, Allan

“…The biosynthesis of histidine is a central metabolic process in organisms ranging from bacteria to yeast and plants. The seventh step in the synthesis of…”
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The crystal structure of Escherichia coli MoeA, a protein from the molybdopterin synthesis pathway by Schrag, Joseph D, Huang, Weijun, Sivaraman, J, Smith, Christopher, Plamondon, Josée, Larocque, Robert, Matte, Allan, Cygler, Miroslaw

“…MoeA is involved in synthesis of the molybdopterin cofactor, although its function is not yet clearly defined. The three-dimensional structure of the…”
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