Effect-directed fractionation and identification of cytochrome P4501A-inducing halogenated aromatic hydrocarbons in a contaminated sediment

Effect-directed fractionation and identification of cytochrome P4501A-inducing halogenated aromatic hydrocarbons in a contaminated sediment

On the basis of a new fractionation method combined with in vitro ethoxyresorufin‐O‐deethylase (EROD) induction in a rainbow trout liver cell line (RTL‐W1) and chemical analysis, halogenated aromatic hydrocarbons with dioxin‐like activity were identified in a sediment extract from Bitterfeld, German...

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Bibliographic Details
Published in:Environmental toxicology and chemistry Vol. 21; no. 12; pp. 2654 - 2662
Main Authors: Brack, Werner, Schirmer, Kristin, Kind, Tobias, Schrader, Steefi, Schüürmann, Gerrit
Format: Journal Article
Language:English
Published: Hoboken Wiley Periodicals, Inc 01-12-2002
Subjects:
Animals
Benzofurans - analysis
Benzofurans - pharmacology
Cell Line
CYP1A protein
Cytochrome P-450 CYP1A1 - biosynthesis
Cytochrome P-450 CYP1A1 - pharmacology
cytochrome P4501A
Dibenzofurans, Polychlorinated
Effect-directed fractionation
Environmental Monitoring - methods
Environmental Pollutants - analysis
Environmental Pollutants - pharmacology
Enzyme Induction
Ethoxyresorufin-O-deethylase
Geologic Sediments - chemistry
Germany, Bitterfeld
halogenated aromatic hydrocarbons
Liver - enzymology
Naphthalenes - analysis
Naphthalenes - pharmacology
Oncorhynchus mykiss - physiology
Polychlorinated Biphenyls - analysis
Polychlorinated Biphenyls - pharmacology
Polychlorinated Dibenzodioxins - analogs & derivatives
Polychlorinated Dibenzodioxins - analysis
Polychlorinated Dibenzodioxins - pharmacology
Sediment extract
Soil Pollutants - analysis
Soil Pollutants - pharmacology
Toxicant identification
Germany, Sachsen-Anhalt. Bitterfeld
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Summary:On the basis of a new fractionation method combined with in vitro ethoxyresorufin‐O‐deethylase (EROD) induction in a rainbow trout liver cell line (RTL‐W1) and chemical analysis, halogenated aromatic hydrocarbons with dioxin‐like activity were identified in a sediment extract from Bitterfeld, Germany. The fractionation method allowed a separation of different nonplanar and coplanar polychlorinated biphenyls (PCBs), polychlorinated naphthalenes (PCNs), dibenzo‐p‐dioxins (PCDDs), and dibenzofurans (PCDFs) with different degrees of chlorination. The dioxin‐like activity at the investigated site could be quantitatively assigned to PCDD/Fs. Both PCBs and PCNs could be excluded as the cause of the measured effects on the basis of the fractionation procedure and bioanalytical results. Thus, the method allowed the chemical analysis to focus on PCDD/Fs, with significant reduction of the analytical expense. The EROD‐induction potency of sediment‐extract fractions was quantified, and toxicants were confirmed by the application of induction equivalent quantities on the basis of fixed‐effect‐level concentrations that exhibit 15% of the maximum induction by 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin. This approach was designed to minimize methodological limitations due to superimposing inhibitory effects.
Bibliography:ark:/67375/WNG-KFGPHW4B-X
istex:2E61B1F5C4950AD1D46C7058B178473A947BC1CE
ArticleID:ETC5620211218
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0730-7268
1552-8618
DOI:10.1002/etc.5620211218