Search Results - "Schleef, Martin"

Systemic Exosomal Delivery of shRNA Minicircles Prevents Parkinsonian Pathology by Izco, María, Blesa, Javier, Schleef, Martin, Schmeer, Marco, Porcari, Riccardo, Al-Shawi, Raya, Ellmerich, Stephan, de Toro, María, Gardiner, Chris, Seow, Yiqi, Reinares-Sebastian, Alejandro, Forcen, Raquel, Simons, J. Paul, Bellotti, Vittorio, Cooper, J. Mark, Alvarez-Erviti, Lydia

“…The development of new therapies to slow down or halt the progression of Parkinson’s disease is a health care priority. A key pathological feature is the…”
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Use of minicircle plasmids for gene therapy by Mayrhofer, Peter, Schleef, Martin, Jechlinger, Wolfgang

“…A large number of cancer gene therapy clinical trials are currently being performed that are attempting to evaluate novel approaches to eliminate tumor cells…”
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Targeted Extracellular Vesicle Gene Therapy for Modulating Alpha-Synuclein Expression in Gut and Spinal Cord by Izco, Maria, Schleef, Martin, Schmeer, Marco, Carlos, Estefania, Verona, Guglielmo, Alvarez-Erviti, Lydia

“…The development of effective disease-modifying therapies to halt Parkinson's disease (PD) progression is required. In a subtype of PD patients, alpha-synuclein…”
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Correction to: Recombinant expression of Barnase in Escherichia coli and its application in plasmid purifcation by Shankar, Ram, Schäfer, Nina, Schmeer, Marco, Risse, Joe Max, Friehs, Karl, Schleef, Martin

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Recombinant expression of Barnase in Escherichia coli and its application in plasmid purification by Shankar, Ram, Schäffer, Nina, Schmeer, Marco, Risse, Joe Max, Friehs, Karl, Schleef, Martin

“…The use of bovine-origin ribonucleases has been part of the standard protocol for plasmid DNA purification. As the field of gene therapy now enters the…”
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Automated, scaled, transposon-based production of CAR T cells by Lock, Dominik, Monjezi, Razieh, Brandes, Caroline, Bates, Stephan, Lennartz, Simon, Teppert, Karin, Gehrke, Leon, Karasakalidou-Seidt, Rafailla, Lukic, Teodora, Schmeer, Marco, Schleef, Martin, Werchau, Niels, Eyrich, Matthias, Assenmacher, Mario, Kaiser, Andrew, Prommersberger, Sabrina, Schaser, Thomas, Hudecek, Michael

“…BackgroundThere is an increasing demand for chimeric antigen receptor (CAR) T cell products from patients and care givers. Here, we established an automated…”
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Self-Complementary Adeno-Associated Virus Vectors Improve Transduction Efficiency of Corneal Endothelial Cells by Gruenert, Anja K, Czugala, Marta, Mueller, Chris, Schmeer, Marco, Schleef, Martin, Kruse, Friedrich E, Fuchsluger, Thomas A

“…Transplantation of a donor cornea to restore vision is the most frequently performed transplantation in the world. Corneal endothelial cells (CEC) are crucial…”
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Effect of the Compaction and the Size of DNA on the Nuclear Transfer Efficiency after Microinjection in Synchronized Cells by Akita, Hidetaka, Kurihara, Dai, Schmeer, Marco, Schleef, Martin, Harashima, Hideyoshi

“…The nuclear transfer process is one of the critical rate-limiting processes in transgene expression. In the present study, we report on the effect of…”
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Low-Dose Gene Therapy for Murine PKU Using Episomal Naked DNA Vectors Expressing PAH from Its Endogenous Liver Promoter by Grisch-Chan, Hiu Man, Schlegel, Andrea, Scherer, Tanja, Allegri, Gabriella, Heidelberger, Raphael, Tsikrika, Panagiota, Schmeer, Marco, Schleef, Martin, Harding, Cary O., Häberle, Johannes, Thöny, Beat

“…Limited duration of transgene expression, insertional mutagenesis, and size limitations for transgene cassettes pose challenges and risk factors for many gene…”
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DNA Minicircle Technology Improves Purity of Adeno-associated Viral Vector Preparations by Schnödt, Maria, Schmeer, Marco, Kracher, Barbara, Krüsemann, Christa, Espinosa, Laura Escalona, Grünert, Anja, Fuchsluger, Thomas, Rischmüller, Anja, Schleef, Martin, Büning, Hildegard

“…Adeno-associated viral (AAV) vectors are considered as one of the most promising delivery systems in human gene therapy. In addition, AAV vectors are…”
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Animal-free production of ccc-supercoiled plasmids for research and clinical applications by Schleef, Martin, Schmidt, Torsten

“…The topological structure of plasmid DNA can be characterized by capillary gel electrophoresis (CGE analysis)—an important tool for quality control and…”
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Novel Jet-Injection Technology for Nonviral Intratumoral Gene Transfer in Patients with Melanoma and Breast Cancer by WALTHER, Wolfgang, SIEGEL, Robert, SCHLAG, Peter M, KOBELT, Dennis, KNÖSEL, Thomas, DIETEL, Manfred, BEMBENEK, Andreas, AUMANN, Jutta, SCHLEEF, Martin, BAIER, Ruth, STEIN, Ulrike

“…Purpose: This phase I clinical trial evaluated safety, feasibility, and efficiency of nonviral intratumoral jet-injection gene transfer in patients with skin…”
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Production of non viral DNA vectors by Schleef, Martin, Blaesen, Markus, Schmeer, Marco, Baier, Ruth, Marie, Corinne, Dickson, George, Scherman, Daniel

“…After some decades of research, development and first clinical approaches to use DNA vectors in gene therapy, cell therapy and DNA vaccination, the…”
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Transgene expression of transfected supercoiled plasmid DNA concatemers in mammalian cells by Maucksch, Christof, Bohla, Alexander, Hoffmann, Florian, Schleef, Martin, Aneja, Manish Kumar, Elfinger, Markus, Hartl, Dominik, Rudolph, Carsten

“…Background Supercoiled topology of transfected plasmid DNA (pDNA) is critical for transgene expression in mammalian cells. In the present study, we analysed…”
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Plasmid DNA Manufacturing for Indirect and Direct Clinical Applications by Schmeer, Marco, Buchholz, Tatjana, Schleef, Martin

“…Plasmid DNA is currently gaining increasing importance for clinical research applications in gene therapy and genetic vaccination. For direct gene transfer…”
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Effect of ammonium chloride on plasmid DNA production in high cell density batch culture for biopharmaceutical use by Voss, Carsten, Schmidt, Torsten, Schleef, Martin, Friehs, Karl, Flaschel, Erwin

“…In this paper the influence of ammonium salt concentration on the production of pharmaceutical grade plasmid DNA from unfed high cell density batch culture on…”
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Pharmaceutical grade large-scale plasmid DNA manufacturing process by Schmeer, Marco, Schleef, Martin

“…For pharmaceutical applications of plasmid DNA, either direct or indirect, certain quality standards are required. Whereas for direct gene transfer into human…”
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Producing Plasmid DNA Template for Clinical Grade RNA Vaccine Manufacture by Shankar, Ram, Schmeer, Marco, Schleef, Martin

“…A plasmid production process has been established to manufacture plasmid DNA at a large scale in High-Quality grade. This is used as a starting material to…”
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Minicircle-DNA production by site specific recombination and protein-DNA interaction chromatography by Mayrhofer, Peter, Blaesen, Markus, Schleef, Martin, Jechlinger, Wolfgang

“…Background Conventional plasmid‐DNA (pDNA) used in gene therapy and vaccination can be subdivided into a bacterial backbone and a transcription unit. Bacterial…”
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465. An Antibiotic-Free Strategy for Miniplasmid Production by Shankar, Ram, Telaar, Maurice, Friehs, Karl, Schleef, Martin, Flaschel, Erwin

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