Search Results - "Schlatterer, C"

Mapping the kinetic barriers of a Large RNA molecule's folding landscape by Schlatterer, Jörg C, Martin, Joshua S, Laederach, Alain, Brenowitz, Michael

“…The folding of linear polymers into discrete three-dimensional structures is often required for biological function. The formation of long-lived intermediates…”
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Conformational heterogeneity of the protein-free human spliceosomal U2-U6 snRNA complex by Zhao, Caijie, Bachu, Ravichandra, Popovic, Milena, Devany, Matthew, Brenowitz, Michael, Schlatterer, Jörg C, Greenbaum, Nancy L

“…The complex formed between the U2 and U6 small nuclear (sn)RNA molecules of the eukaryotic spliceosome plays a critical role in the catalysis of precursor mRNA…”
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Pyrite footprinting of RNA by Schlatterer, Jörg C., Wieder, Matthew S., Jones, Christopher D., Pollack, Lois, Brenowitz, Michael

“…► RNA structure is mapped by pyrite mediated OH footprinting. ► Repetitive experiments can be done in a powdered pyrite filled cartridge. ► High OH reactivity…”
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Complementing global measures of RNA folding with local reports of backbone solvent accessibility by time resolved hydroxyl radical footprinting by Schlatterer, Jörg C., Brenowitz, Michael

“…A variety of analytical techniques are used to probe the mechanisms by which RNA molecules fold to discrete three dimensional structures. Methods such as small…”
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Hinge Stiffness Is a Barrier to RNA Folding by Schlatterer, Jörg C., Kwok, Lisa W., Lamb, Jessica S., Park, Hye Yoon, Andresen, Kurt, Brenowitz, Michael, Pollack, Lois

“…Cation-mediated RNA folding from extended to compact, biologically active conformations relies on a temporal balance of forces. The Mg 2 + -mediated folding of…”
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A microfluidic device that generates hydroxyl radicals to probe the solvent accessible surface of nucleic acids by Jones, Christopher D, Schlatterer, Jörg C, Brenowitz, Michael, Pollack, Lois

“…We describe a microfluidic device containing a mineral matrix capable of rapidly generating hydroxyl radicals that enables high-resolution structural studies…”
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NSET Molecular Beacon Analysis of Hammerhead RNA Substrate Binding and Catalysis by Jennings, T. L, Schlatterer, J. C, Singh, M. P, Greenbaum, N. L, Strouse, G. F

“…Nanometal surface energy transfer (NSET), which describes an energy transfer event from optically excited organic fluorophores to small metal nanoparticles,…”
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DNA damage-site recognition by lysine conjugates by Breiner, Boris, Schlatterer, Jörg C, Alabugin, Igor V, Kovalenko, Serguei V, Greenbaum, Nancy L

“…Simple lysine conjugates are capable of selective DNA damage at sites approximating a variety of naturally occurring DNA-damage patterns. This process…”
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Universal initiator nucleotides for the enzymatic synthesis of 5′-amino- and 5′-thiol-modified RNA by Schlatterer, Jörg C., Jäschke, Andres

“…We report the chemical synthesis of 5′-amino- and 5′-thiol-hexaethylene glycol guanosine nucleotides and their enzymatic incorporation into RNA, followed by…”
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Monitoring equilibrium changes in RNA structure by 'peroxidative' and 'oxidative' hydroxyl radical footprinting by Bachu, Ravichandra, Padlan, Frances-Camille S, Rouhanifard, Sara, Brenowitz, Michael, Schlatterer, Jörg C

“…RNA molecules play an essential role in biology. In addition to transmitting genetic information, RNA can fold into unique tertiary structures fulfilling a…”
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Stereoselective Synthesis using Immobilized Diels-Alderase Ribozymes by Schlatterer, Jörg C, Stuhlmann, Friedrich, Jäschke, Andres

“…Development of artificial ribozymes by in vitro selection has so far, mostly been addressed from the viewpoint of fundamental research. However, such ribozymes…”
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Protein footprinting by pyrite shrink-wrap laminate by Leser, Micheal, Pegan, Jonathan, El Makkaoui, Mohammed, Schlatterer, Joerg C, Khine, Michelle, Law, Matt, Brenowitz, Michael

“…The structure of macromolecules and their complexes dictate their biological function. In "footprinting", the solvent accessibility of the residues that…”
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Conformation of the Group II Intron Branch Site in Solution by Schlatterer, Jörg C, Crayton, Samuel H, Greenbaum, Nancy L

“…Group II introns are multidomain ribozymes that catalyze their own removal from pre-mRNA. The nucleophile for the first cleavage step is the 2‘OH of a specific…”
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Calcium stores in differentiated Dictyostelium discoideum: prespore cells sequester calcium more efficiently than prestalk cells by Schlatterer, C., Walther, P., Müller, M., Mendgen, K., Zierold, Karl, Knoll, G.

“…Dictyostelium discoideum pseudoplasmodia exhibit a gradient of the cytosolic free Ca2+-concentration ([Ca2+]i) along their anterior–posterior axis involved in…”
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Intracellular calcium during chemotaxis of Dictyostelium discoideum: a new fura-2 derivative avoids sequestration of the indicator and allows long-term calcium measurements by Schlatterer, C, Knoll, G, Malchow, D

“…During stimulation of Dictyostelium discoideum amoebae with the chemoattractant cAMP, extracellular calcium is taken up by the cells. The aim of this study was…”
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An increase in cytosolic Ca2+ delays cAMP oscillations in Dictyostelium cells by Malchow, D, Schaloske, R, Schlatterer, C

“…We have shown that calmidazolium (R24571) causes a transient increase in the cytosolic free Ca2+ concentration ([Ca2+]i) in Dictyostelium discoideum…”
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Calcium-sequestering organelles of Dictyostelium discoideum: changes in element content during early development as measured by electron probe X-ray microanalysis by Schlatterer, C, Buravkov, S, Zierold, K, Knoll, G

“…Starving Dictyostelium discoideum amoebae aggregate within a few hours by chemotaxis towards the attractant cAMP to form a multicellular organism. The…”
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Specificity of Mg2+ binding at the Group II intron branch site by Schlatterer, Jörg C, Greenbaum, Nancy L

“…Metal ions play a crucial role in the conformation and splicing activity of Group II introns. Results from 2-aminopurine fluorescence and solution NMR studies…”
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Protected 32P‐Labels in Deoxyribonucleotides: Investigation of Sequence Selectivity of DNA Photocleavage by Enediyne–, Fulvene–, and Acetylene–Lysine Conjugates by Breiner, Boris, Schlatterer, Jörg C., Kovalenko, Serguei V., Greenbaum, Nancy L., Alabugin, Igor V.

“…Finding a compromise: The sequence selectivity of DNA photocleavage by enediyne–, acetylene–, and fulvene–lysine conjugates was investigated by using labeled…”
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Purification of prostaglandin D synthase by ceramic- and size exclusion chromatography by Schlatterer, J.C., Baeker, R., Schlatterer, B., Klose, J., Kehler, W., Schlatterer, K.

“…Prostaglandin D synthase (L-PGDS) is a major glycosylated polypeptide in cerebrospinal fluid (CSF). The overexpression of L-PGDS in inflamed bovine mammary…”
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